Abstract
In the past four years there has been a distinct tendency to abandon the earlier methods of enzyme histochemistry that were characterized by a general and diffuse cytoplasmic localization and to direct more interest and attention to methods capable of demonstrating mitochondria or other organelles. Disenchantment with former methods has undoubtedly been accelerated by the perfection of methods for the dehydrogenases, which demonstrate mitochondria clearly and readily [38, 59, 60]. It has been finally recognized, that much of the diffuseness obtained with hydrolytic enzymes was due to the soluble and diffusible component of the enzymes (lyoenzyme). By using various methods of fixation this component could be effectively allowed to diffuse from the tissue leaving behind for histochemical demonstration the more precisely localized, less soluble component of the enzymes (desmoenzyme) [36], made even more insoluble by appropriate fixation. The use of better fixation, better substrates and enzyme inhibitors has been instrumental in delineating the droplet form of some hydrolytic enzymes, and these localizations have been correlated with de Duvr’s interesting and important biochemical work on the lysosomes [11, 23, 40]. Most popular have become methods for various dehydrogenases using the ditetrazolium hydrogen acceptor, nitro-BT [57] and for cytochrome oxidase using analogues of phenylene diamine and naphtholic derivatives [7, 33]. The methods for the hydrolytic enzymes have been improved by using formalinfixed tissue [51] and substrates and coupling agents that yield insoluble and substantive dye pigments, such for example as the demonstration of esterase with fast blue BB and naphthol-AS acetate, a substrate first proposed by Dr. Gomori [16]. Much effort has been directed with success toward proper fixation in order to preserve organelle morphology [12, 18, 59, 60] while maintaining significant enzymatic activity and at the same time allowing diffusible enzyme to leave the tissue block.
This work was supported by a research grant (CA-02478-10) from the National Cancer Institute, National Institutes of Health, Department of Health, Education and Welfare, Bethesda, Maryland.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
Bibliography
Abe, M., S. P. Kramer, and A. M. Seligman: J. Histochem. Cytochem. 12, 364–383 (1964).
Barrnett, R. J.: In: Fourth Internat. Conference on Electron Microscopy, vol. II p. 91–100, 1960.
Barrnett, R. J., R. Bressler, and A. M. Rutenburg: Anat. Rec. 124, 255–256 (1956).
Barrnett, R. J., R. Bressler, A. M. Rutenburg, and G. E. Palade: J biophys. biochem. Cytol. 3, 577–588 (1957).
Barrnett, R. J., R. Bressler, A. M. Rutenburg, and G. E. Palade: J. biophys. biochem. Cytol. 6, 163–169 (1959).
Burstone, M. S.: J. Histochem. Cytochem. 2, 88–94 (1954).
Burstone, M. S.: J. Histochem. Cytochem. 7, 112–122 (1959).
Burstone, M. S.: J. nat. Cancer Inst. 18, 167–172 (1957).
Burstone, M. S.: J. nat. Cancer Inst. 21, 523–539 (1958).
Crevier, M., and L. F. BÉLanger: Science 122, 556 (1955).
Duve, C. DE: In: Subcellular particles ( T. Mihyashi ed.), p. 128–158. New York: Ronald Press 1959.
ERÄNKÖ, O.: Acta anat. (Basel) 16 Suppl. 17, 3–60 (1952).
Freeman, J. A., and B. O. Spurlock: J. Cell Biol. 13, 437–443 (1962).
Friedman, O. M., and A. M. Seligman: J. Amer. chem. Soc. 72, 624–625 (1950).
Goldfischer, S., E. Essner, and A. B. Novikoff: J. Histochem. Cytochem. 12, 72–95 (1964).
Gomori, G.: Int. Rev. Cytol. 1, 323–335 (1952).
Gomori, G.: J. Lab. clin. Med. 35, 802–809 (1950).
Gomori, G.: Microscopic Histochemistry; Principles and Practice. Chicago, Ill.: Chicago Univ. Press 1952.
Gomori, G.: Stain Technol. 25, 81–85 (1950).
Hanker, J., A. Seaman, L. Weiss, H. Ueno, H. Dmochowski, L. Katzoff, and A. M. Seligman: J. Histochem. Cytochem. (in press) (1964).
H. Storm, H. Wasserkrug, L. Katzoff, and A. M. Seligman: Science (in press) (1964).
H. Storm, H. Wasserkrug, L. Katzoff, and A. M. Seligman, L. Weiss, H. Dmochowski, L. Katzoff, and A. M. Seligman: J. Histochem. Cytochem. (in press) (1964).
Holt, S. J.: Exp. Cell Res., Suppl., 7, 1–27 (1959).
Holt, S. J., and R. M. Hicks: J. biophys. biochem. Cytol. 11, 47–66 (1961).
Holt, S. J., J. Histochem. Cytochem. 4, 541–552 (1956).
Holt, S. J., and R. F. J. Withers: Nature (Lond.) 170, 1012–1014 (1952).
Karnovsky, M. J.: J. biochem. biophys. 11, 729–732 (1961).
Holt, S. J., and K. Hug: J. Cell Biol. 19, 255–260 (1963).
Komma, D. J.: J. Histochem. Cytochem. 11, 619–623 (1963).
Lehrer, G. M., and L. Ornstein: J. biophys. biochem. Cytol. 6, 399–406 (1959).
MöLbert, E., F. Dusfiva, and O. H. v. Deimling: J biophys. biochem. Cytol. 7, 387–390 (1960).
Moog, F.: J. cell. comp. Physiol. 22, 223–231 (1943).
Nachlas, M. M., D. T. Crawford, T. P. Goldstein, and A. M. Seligman: J. Histochem. Cytochem. 6, 445–456 (1958).
Nachlas, M. M., D. T. Crawford, T. P. Goldstein, T. P. Goldstein, D. H. Rosenblatt, M. Kirsch, and A. M. Seligman: J. Histochem. Cytochem. 7, 50–65 (1959).
Nachlas, M. M., D. T. Crawford, T. P. Goldstein, B. Monis, D. Rosenblatt, and A. M. Seligman: J. biophys. biochem. Cytol. 7, 261–264 (1960).
Nachlas, M. M., D. T. Crawford, T. P. Goldstein, W. Prinn, and A. M. Seligman: J. biophys. biochem. Cytol. 2, 487–502 (1956).
Nachlas, M. M., D. T. Crawford, T. P. Goldstein,, and A. M. Seligman: J. nat. Cancer Inst. 9, 415–425 (1949).
Nachlas, M. M., D. T. Crawford, T. P. Goldstein, K. C. Tsou, E. DE Souza, C. S. Cheng, and A. M. Seligman: J. Histochem. Cytochem. 5, 420–436 (1957).
Nachlas, M. M., D. T. Crawford, T. P. Goldstein, A. C. Young, and A. M. Seligman: J. Histochem. Cytochem. 5, 565–583 (1957).
NovIkoff, A. B., and E. Essner: Amer. J. Med. 29, 102–131 (1960).
Ogawa, K., and Y. Saito: J. Electron Mier. (Japan) 12, 68–71 (1963).
Pearse, A. G. E.: Histochemistry, Theoretical and Applied. p. 442–443. Little Brown, and Co. 1960.
Pearse, A. G. E.: Int. Rev. Cytol. 3, 329–358 (1954).
Pugh, D., and P. G. Walker: J. Histochem. Cytochem. 9, 105–106 (1961).
Rutenburg, A. M., and A. M. Seligman: J. Histochem. Cytochem. 3, 455–470 (1955).
Sabatini, D. D., K. Bensch, and R. J. Barrnett: J. Cell Biol. 17, 19–58 (1963).
Sabatini, D. D., K. Bensch, and R. J. Barrnett, F. Miller, and R. J. Barrnett: J. Histochem. Cytochem. 12, 57–71 (1964).
Seaman, A., J. Hanker, H. Storm, H. Dmochowski, L. Katzoff, and A. M. Seligman:
J. Cell Biol. (in press) (1964).
Seaman, A. R., J. S. Hanker, and A. M. Seligman: J. Histochem. Cytochem. 9, 596–597 (1961).
Sedar, A. W., and C. G. RosA: Anat. Rec. 130, 371 (1958).
Seligman, A. M., H. H. Chauncey, and M. M. Nachlas: Stain Technol. 26, 19–23 (1951).
Seligman, A. M., H. H. Chauncey,, and L. H. Manheimer: J nat. Cancer Inst. 6, 427–434 (1949).
Seligman, A. M., H. H. Chauncey, and A. M. Rutenburg: J. Amer. them. Soc. 72, 3214–3216 (1950).
Seligman, A. M., H. H. Chauncey, and A. M. Rutenburg: Sinai Hosp. J. (Baltimore) 5, 90–102 (1956).
Sheldon, H., H. Zetterquist, and D. Brandes: Exp. Cell Res. 9, 592–596 (1955).
Shnitka, T. K., and A. M. Seligman: J. Histochem. Cytochem. 9, 504–527 (1961).
Tsou, K. C., C. S. Cheng, M. M. Nachlas, and A. M. Seligman: J. Amer. them. Soc. 78, 6139–6144 (1956).
Wachstein, M., and E. Meisel: J. Histochem. Cytochem. 8, 317–318 (1960).
Walker, D. G., and A. M. Seligman: J. biophys. biochem. Cytol. 9, 415–427 (1961).
Walker, D. G., and A. M. Seligman: J. Cell Biol. 16, 455–469 (1963).
Ahlqvist, J.: Acta path. microbiol. scand. 59, 171 (1963).
Barka, T.: Nature (Lond.) 187, 248 (1960).
Burstone, M. S.: J. Histochem. Cytochem. 9, 146 (1961).
Cameron, G. R., and S. Muzaffar Hasan: J. Path. Bact. 75, 333 (1958).
Cobb, J.: Anat. Rec. 103, 531 (1949).
Dallner, G.: Acta path. microbiol. scand., Suppl. 166 (1963).
Daoust, R.: Exp. Cell. Res. 12, 303 (1957).
Daoust, R.: Exp. Cell. Res., Suppl. 7, 40 (1959).
Eiduson, B. T.: Scientists. Their psychological world. New York: Basic Books 1962.
Hunter, R., and G. Markert: Science 125, 1294 (1957).
Koelle, G. B.: J. comp. Neurol. 100, 211 (1954).
Kubie, L. S.: Amer. Scientist 41, 596 (1953).
Lojda, Z., B. Vecerek, and H. Pelichova: Histochemie 3, 428 (1964).
Niles, N. R., L. Bitensky, J. Chayen, G. J. Cunningham, and M. W. Braimbridge: Lancet 1964 I, 963.
Patterson, E. K., S.-H. HsIao, and A. Keppel: J. biol. Chem. 238, 3611 (1963).
Pinkerton, J. H. M., D. G. McKay, E. C. Adams, and A. T. Hertig: Obstet. and Gynec. 18, 152 (1961).
Shute, C. C. D., and P. R. Lewis: Nature (Lond.) 199, 1160 (1963).
SylvÉN, B., and I. Bois: Histochemie 3, 65 (1962).
SylvÉN, B., and I. Bois: Histochemie 3, 341 (1963).
SylvÉN, B., and D. Snellman: Histochemie 3, 484 (1964).
Takamatsu, H., and K. Wada: Trans. Soc. path. jap. 43, 204 (1954).
SylvÉN, B., and I. Bois, and F. YosHino: Trans. Soc. path. jap. 43 (1954).
Takeuchi, T., and H. Kuriaki: J Histochem. Cytochem. 3, 153 (1955).
Virchow, R.: (1858) Cellular pathology. English translation by F. Chance. London: Churchill 1860.
Willighagen, R. G. J., R. O. Van Der Heul, and TH. G. Van Rijssel: J. Path. Bact. 85, 279 (1963).
Willighagen, R. G. J., R. O. Van Der Heul, and H. T. Planteydt: Nature (Lond.) 183, 263 (1959).
Yin, H. C., and C. N. Sun: Science 105, 650 (1947).
YosxiNo, F.: Proc. Jap. Hist. Ass. 1, 94 (1960).
Young, J. Z.: Endeavour 15, 5 (1956).
Albersheim, P., and Ursula Killias: Histochemical localization at the electron microscope level. Amer. J. Bot. 50, 732–745 (1963).
Albersheim, P., and Ursula Killias:, D. MÎ Hlethaler, and A. Frey-Wyssling: Stained pectin as seen in the electron microscope. J. biophys. biochem. Cytol. 8, 501–516 (1960).
Bell, P. R.: Failure of nucleotides to diffuse freely into the embryo of Pteridium aquilinum. Nature (Lond.) 191, 91 (1961).
Kuhn. Proc. roy. Soc. B 153, 421 (1961).
Bell, P. R.: The cytochemical and ultrastructural peculiarities of the fern egg. J. Linnean Soc. Bot. 58, 353 (1963).
Bell, P. R., and K. MÜHlethaler: A membrane peculiar to the egg in the gametophyte of Pteridium aquilinum. Nature (Lond.) 195, 198 (1962).
Kuhn. J. Ultrastruct. Res. 7, 452 (1962).
Bell, P. R.: The degeneration and reappearance of mitochondria in the egg cells of a plant. J. Cell Biol. 20, 235–248 (1964).
Branton, D., and L. Jacobson: Freeze-drying of plant material. Exp. Cell Res. 22, 559–568 (1961).
Gersh, I.: The Altmann technique for fixation by drying while freezing. Anat. Rec. 53, 309 (1932).
Gifford jr., E. M.: Incorporation of H3–thymidine into shoot and root apices of Ceratopteris. Amer. J. Bot. 47, 834–838 (1960).
Gifford jr., E. M.: Developmental studies of vegetative and floral meristems. Brookhaven Symposia in Biol. 16, 126–136 (1963).
Gifford jr., E. M., and H. B. Teppen: Ontogeny of the inflorescence in Chenopodium album. Amer. J. Bot. 48, 657–666 (1961).
Histochemical and autoradiographic studies of floral induction in Chenopodium. Amer. J. Bot. 49, 706–714 (1962).
Goodspeed, T. H., and F. M. Uber: Application of the Altmann freezing-drying techniques to plant cytology. Proc. nat. Acad. Sci. (Wash.) 20, 495–501 (1934).
Halperin, W., and D. F. Wetherell: Adentive embryony in the tissue cultures of the wild carrot, Daucus corota. Amer. J. Bot. 51, 274–283 (1964).
Jacobson, Ann B., H. Swift, and L. Bogorad: Cytochemical studies concerning the occurrence and distribution of Rna in plastids of Zea mays. J. Cell Biol. 17, 557–570 (1963).
Jensen, W. A.: A new approach to freeze-drying of tissue. Exp. Cell Res. 7, 572–574 (1954).
Jensen, W. A.: The application of freeze-dry methods to plant material. Stain Technol. 29, 143–150 (1954).
Jensen, W. A.: The composition of the developing primary wall in onion root tip cells. II. Cytochemical localization. A.er. J. Bot. 47, 287–295 (1960).
Jensen, W. A.: Botanical histochemistry. San Francisco: W. H. Freeman, and Co. 1962.
Jensen, W. A.: Cell development during plant embryogenesis. Brookhaven Symposia in Biol. 16, 179–202 (1963).
Ledbetter, M. C., and K. R. Porter: A “microtubule” in plant cell fine structure. J. Cell Biol. 19, 239–250 (1963).
Mascarenhas, J. P., and L. Machlis: Chemotropic response of the pollen of Antirrhinum ma jus to calcium. Plant Physiol. 39, 70–77 (1964).
Mollenhauer, H.: Permanganate fixation of plant cells. J. biophys. biochem. Cytol. 2, 215–218 (1959).
MüHlethaler, K., u. P. R. Bell: Untersuchungen über die Kontinuität von Plastiden und Mitochondrien in der Eizelle von Pteridium aquilinum (L.) Kuhn. Naturwissenschaften 49, 63 (1962).
Porter, K. R., and R. D. Machado: Studies on the endoplasmic reticulum. IV. Its form and distribution during mitosis in cells of onion root tip. J. biophys. biochem. Cytol. 7, 167–180 (1953).
Pritchard, H. N.: A cytochemical study of embryo sac development in Stellaria media. Amer. J. Bot. (In press).
Reeve, R. M.: A specific hydroxylamine-ferric chloride reaction for histochemical localization of pectin. Stain Technol. 34, 209–211 (1959).
Rosen, W. G., S. R. Gawlik, W. V. Dasher, and K. A. Siegesmund: Fine structure and cytochemistry of Lilium pollen tubes. Amer. J. Bot. 51, 61–71 (1964).
Steward, F. C.: Growth and organization in free cell cultures. Brookhaven Symposia in Biol. 16, 73–88 (1963).
Takao, A.: Histochemical studies on the formation of some leguminous seeds. Jap. J. Bot. 18, 55–72 (1962).
Van Fleet, D. S.: Histochemical localization of enzymes in vascular plants. Bot. Rev. 18, 354–398 (1952).
Van Fleet, D. S.: Histochemistry of enzymes in plant tissues. In: Handbuch der Histochemie, Bd. Vii/2, S. 1–38. 1962.
Ficq, A., and C. Pavan: Nature (Lond.) 180, 983 (1957).
Lima-DE-Farta, A.: J. biophys. biochem. Cytol. 6, 457 (1959).
Mcmaster-Kaye, R.: J. biophys. biochem. Cytol. 8, 365 (1960).
Plaut, W.: J. molec. Biol. 7, 632 (1963).
Rudrin, G., and S. Corlette: Proc. nat. Acad. Sci. (Wash.) 43, 964 (1957).
Swift, H.: J. biophys. biochem. Cytol. 2 (Suppl.), 415 (1956).
Swift, H.: From: Molecular control of cellular activity, p. 73. Ed. J. Allen: Mc Graw Hill Book Co. 1962.
Taylor, J. H.: J. biophys. biochem. Cytol. 7, 455 (1960).
Woodard, J., E. Rasch, and H. Swift: J. biophys. biochem. Cytol. 9, 445 (1961).
Alfert, M.: Chemie der Genetik, S. 73–84. Berlin-Göttingen-Heidelberg: Springer 1959.
Alfert, M., H. A. Bern u. R. H. Kahn: Acta anat. (Basel) 23, 185 (1955).
Alfert, M., I. I. Geschwind: Proc. Nat. Acad. Sei. (Wash.) 39, 991 (1953).
Allfrey, V. G., M. M. Daly and A. E. Mirsky: J. gen. Physiol. 38, 415 (1955).
Baltus, E.: Biochim. biophys. Acta (Amst) 15, 263 (1954).
Black, M. M., and H. R. Ansley: Science 143, 693 (1964).
Bloch, D. P.: The cell in mitosis. New York: Acad. Press 1961.
Bloch, D. P.: (a) Proc. nat. Acad. Sci. (Wash.) 48, 324 (1962).
Bloch, D. P.: (b) J. Histochem. Cytochem. 10, 137 (1962).
Bloch, D. P., and G. C. Godman: J. biophys. biochem. Cytol. 1, 17 (1955).
Bonner, J., and P. Ts’o: The Nucleohistones. San Francisco-London-Amsterdam: Holden-Day, Inc. 1964.
Brunish, R., and J. M. Luck: J. biol. Chem. 198, 621 (1952).
Busch, H., and J. R. Davis: Cancer Res. 18, 1241–1256 (1958).
Busch, H., and J. R. Davis, and D. C. Anderson: Cancer Res. 18, 916 (1958).
Busch, H., and J. R. Davis, W. J. Steele, Cw. Hnilica, and H. Mavioglu: J. cell. comp. Physiol., Suppl. 1 to vol. 62, No 2, 99–110 (1963a).
Busch, H. B., P. Byvoet, and H. R. Adams: Exp. Cell Res., Suppl. 9, 376–386 (1963b).
Buttler, J. A. V.: Expos. ann. Biochim. méd., Ser. XXI, 41 (1959).
Busch, H. B., P. Byvoet, and H. R. Adams: Exp. Cell Res., Suppl. 9, 349 (1963).
Busch, H. B., P. Byvoet, and H. R. Adams, and P. Corm: Biochem. J. 87, 330 (1963).
Busch, H. B., P. Byvoet, and H. R. Adams, and P. F. Davison: Advanc. Enzymol. 18, 161 (1957).
Busch, H. B., P. Byvoet, and H. R. Adams, and D. J. R. Laurence: Brit. J. Cancer 14, 758 (1960).
Caspersson, T., et L. Santesson: Acta radiol. (Stockh.), Suppl. 46, 1 (1942).
Chargaff, E., C. F. C.Ampton, and R. Lipshitz: Nature (Lond.) 172, 289 (1953).
Chauveau, J., Y. Moule, and C. Rouiller: Exp. Cell Res. 11, 317 (1956).
Chorazy, M. R., A. Bendich, E. Borenfreund, and D. J. Hutchinson: J. Cell Biol. 19, 59 (1963).
Crampton, C. F., W. H. Stein, and S. Moore: J. biol. Chem. 225, 363 (1957).
Cruft, H. J., C. M. Mauritzen, and E. Stedman: Nature (Lond.) 174, 580 (1954).
Daly, M. M., V G Allfrey, and A. E. Mirsky: J. gen. Physiol 36. 173 (1962).
Das, N. K., and M. Alfert: Proc. nat. Acad. Sci. (Wash.) 47, 1 (1961).
Davison, P. F.: Biochem. J. 66, 703 (1957).
Desjardin, R., K. Smetana, W. J. Steele, and H. Busch: Cancer Res. 23, 1819 (1953).
Dounce, A. L.: The nucleic acids (Chargaff et Davidson ed.), II, p. 93. Acad. Press 1955.
Dounce, A. L., and N. K. Sarkar: The cell nucleus, p. 206. Butterworth 1960.
Felix, K.: Advanc. Protein Chem. 15, 1 (1960).
Felix, K., H. Fischer, and A. Krekels: Progr. Biophys. 6, 1 (1956).
Ficq, A.: Exp. Cell Res. 9, 286 (1955).
Ficq, A.: Lab. Invest. 8, 237 (1959).
Frenster, J. H., V. G. Allfrey, and A. E. Mirsky: Proc. nat. Acad. Sci. (Wash.) 46, 432 (1960).
Hamer, D: Brit. J. Cancer 5, 130 (1951).
Himes, M. H., A. W. Pollister, and B. C. Moore: J. Histochem. Cytochem. 3, 390 (1955).
Holrrook, D. J., J. H. Evans, and J. L. Irvin: Exp. Cell Res. 28, 120 (1962).
Holrrook, D. J., J. H. Evans, and J. L. Irvin, J. L. Irvin, E. M. Irvin, and J. Rotherham: Cancer Res. 20, 1329 (1960).
Hnilica, L., E. W. Johns, and J. A. V. Butler: Biochem. J. 82, 123 (1962a).
C. W. Taylor, and H. Busch: Fed. Proc. 21, 406 (1962 b).
C. W. Taylor,, H. Mavioglu, and H. BuscH: Fed. Proc. 22, 658 (1963).
Huang, R. C., and J. Bonner: Proc. nat. Acad. Sci. (Wash.) 48, 1216 (1962).
Huang, R. C., J. Bonner, and K. Murray: J. molec. Biol. 8, 54–64 (1964).
Irvin, J. L., D. J. Holbrook, J. H. Evans, H. C. Mcallister, and E. P. Stiles: Exp. Cell. Res., Suppl. 9, 359 (1963).
Johns, E. W., and J. A. V. Butler: Biochem. J. 84, 436 (1962).
KErR, H. M., R. M. S. Smellie, and G. Siebert: Nature (Lond.) 196, 752 (1962).
Kossel, A.: Physiol. Chem. 8, 511 (1884).
Lindner, A., T. Kutkam, Y. R. Sankaranara, R. Rucker, and J. Aradondo: Exp. Cell Res. Suppl. 9, 485 (1963).
Lucy, J. A., and J. A. V. Butler: Nature (Lond.) 174, 32 (1954).
Lucy, J. A., and J. A. V. Butler: Biochim. biophys. Acta (Amst.) 16, 431 (1955).
Mauritzen, C. M., and E. Stedman: Proc. roy. Soc. B 150, 299 (1959).
Mirsky, A. E., and H. Ris: J. gen. Physiol. 31, 1 (1947).
MoNty, K. J., M. Litt, E. R. M. Kay, and A. L. DouxcE: J. biophys. biochem. Cytol. 2, 127 (1956).
Moore, S.: Rapp. Cons; Chim Solvay 11, 77 (1959).
Muramatsu, M., K. Smetana, and H. Busch: Cancer Res. 23, 510 (1963).
Neelin, J. M., et G. E. Connell: Biochim. biophys. Acta (Amst) 31, 539 (1959).
Okuda, J., D. Szafarz, and Y. Khouvine: C.R. Acad. Sci. (Paris) 257, 2904 (1963).
Peacocke, A. R.: Progr. Biophys. 10, 55 (1960).
Perugini, S., U. Torelli et M. Soldati: Experientia (Basel) 13, 441 (1957).
Phillips, D. M. P.: Prog. Biophys. 12, 211 (1962).
Phillips, D. M. P., and P. Simsox: Biochem. J. 82, 236 (1962).
Pogo, A. O., B. G. T. Pogo, V. C. Littau, V. G. Allfrey, A. E. Mirsky et M. G. Hamilton:
Biochem. biophys. Acta (Amst) 55, 849 (1962).
Prescott, D. M., and M. A. Bender: J. Cell comp. Physiol., Suppl. 1, 62, 175 (1963).
Rash, E., and S. W. Woodard: J. biophys. biochem. Cytol. 6, 263 (1959).
Ringertz, N. R.: Exp. Cell. Res. 32, 401 (1963).
Rotherham, J., J. L. Irvix, E. M. IRvIx, and D. J. Holbrook: Proc. Soc. exp. Biol. (N.Y). 96, 21 (1957).
Seed, J.: Nature (Lond.) 198, 147 (1963).
Seed, J.: J. Cell Biol. 20, 17 (1964).
Siebert, G.: Exp. Cell Res., Suppl. 9, 389 (1963).
Smellie, R M S, W. M. McIndoe, et J. N. Davidson: Biochim. biophys. Acta (Amst) 11, 559 (1953).
Smithies, O.: Biochem. J. 61, 629 (1955).
Somers, C. E., A. Cole, and T. C. Hsu: Exp. Cell Res., Suppl. 9, 220 (1963).
Starbruck, W. C., and H. Busch: Cancer Res. 20 (6), 890 (1960).
Stedman, E., and E. Stedman: Symp. Soc. exp. Biol. 1, 232 (1947).
Stedman, E., and E. Stedman: Nature (Lond.) 166, 780 (1950).
Stedman, E., and E. Stedman: Phil. Trans. B 235, 565 (1951).
Steele, W. J., and H. BuscH: Cancer Res. 23, 1153 (1963).
Vendrely, C., A. Knobloch et R. Vendrely: Biochim. biophys. Acta (Amst.) 19, 472 (1956).
Vendrely, R.: The Nucleic acids, vol. II, p. 155. New York: Acad. Press. 1955.
Vendrely, R.: In: The Nucleohistones, (J. Bonner, and P. Ts’o ed.), p. 307. San Francisco-LondonAmsterdam: Holden-Day Inc. 1964.
Vendrely, R., Y. Coirault et A. Vanderplancke: C.R. Acad. Sci. (Paris), sous presse (1964).
Vendrely, R., A. Knobloch, and H. Matsudaira: Nature (Lond.) 181, 343 (1958).
Vendrely, R., A. Knobloch-Mazen, and C. Vendrely: The Cell Nucleus, p. 200. London: Butter-worths Sci. publ. 1960a. Biochim. Pharmacol. 4, 19 (1960b).
Vincent, W. S.: Proc. nat. Acad. Sci. (Wash.) 38, 138 (1952).
Wang, T. Y.: Biochim. biophys. Acta (Amst) 45, 8 (1960).
Zalta, J. P., R. R.zExcwAsg, N. Carasso et P. Fayard: C.R. Acad. Sci. (Paris) 1, 412 (1962).
Zbarsky, I. B., i. G. P. Georgiev: Biokhimiya 24, 192 (1959).
Zbarsky, I. B., N. P. Dmitrieva, and L. P. Yermolaeva: Exp. Cell Res. 27, 573 (1962).
Lowry, O. H.: J. Histochem. Cytochem. 1, 420
Robins, E., D. E. Smith, G. E. Daesch, and K. E. Linderstrom-Lang, K., and K. R. Mogensen: C. 23, 27 (1938).
Lowry, O. H., N. R. Roberts, D. W. Schulz:, J. Chem. 236, 2813 (1961).
Lowry, O. H., N. R. Roberts, D. W. Schulz: Bull. N.Y. Acad. Med. 38, 789 (1962).
Lowry, O. H., N. R. Roberts, D. W. Schulz: Harvey Lect., Series 58, 1 (1963).
Lowry, O. H., N. R. Roberts, D. W. Schulz: J. V. Passonneau, and M. K. RocK: J. Histochem. Cytochem. 8, 335 (1960).
Lowry, O. H., N. R. Roberts, D. W. Schulz: J. Mt Sinai Hosp. 30, 375 (1963).
Glich, D.: Quantitative Chemical Techniques of Histo-and Cytochemistry, vols. I, II. New York and London: Interscience 1962, 1963.
Kaplan, N. O., S. P. CoLowjck, and C. C. Barnes: J. biol. Chem. 191, 461 (1951).
Lowry, O. H., J. V. Passonneau, D. W. Schulz and M. K. Rock: J. biol. Chem. 236, 2746 (1961).
Andresen, N., FR. Engel, and H. Holter: C. R. Lab. C.rlsberg, Sér. chim. 27, 408 (1951).
Andresen, N., and B. M. PoLlocx: C. R. Lab. C.rlsberg, Sér. chim. 28, 247 (1952).
Andresen, N., and C. W. Mushett: C. R. Lab. Carlsberg 33, 265 (1963).
Boell, E. J.: The Cartesian diver technique in microrespirometry and enzyme assay. In: Sasser and Jenkins, Eds. Nematology, pp. 109–121. Chapell Hill: The University of North Carolina Press 1960.
Borrow, A., and J. R. Penney: Exp. Cell Res. 11, 188 (1951).
Brzin, M., and Z. Majcen-Tkacev: J. Cell Biol. 19, 349 (1963).
Brzin, M., and E. Zeuthen: C. R. Lab. Carlsberg 32, 139 (1961).
Chakravarty, N.: C. R. Lab. Carlsberg (1964) (in press).
Chakravarty, N., and E. Zeuthen: C. R. Lab. Carlsberg (1964) (in press).
Frydenberg, O., and E. Zeuthen: C. R. Lab. Carlsberg 31, 423 (1960).
Geilenkirchen, W. L. M.: Effects of mono-and divalent cations on viability and oxygen uptake of eggs of Limnaea stagnalis. Diss. Utrecht 1961, Neerlandia.
Giacobini, E.: Acta physiol. scand. 45, Suppl. 156 (1959).
Giacobini, E.: J. Neurochem. 9, 169 (1962).
Glicx, D.: Quantitative chemical techniques of histo-and cytochemistry, vol. I, pp. 123–289. New York and London: Interscience Publishers 1961.
Gonse, P. H.: Exp. Cell Res. 8, 550 (1955).
Gonse, P. H.: Biochim. biophys. Acta (Amst.) 24, 267, 520 (1957).
Gregg, J. R., and S. Lovtrup: C. R. Lab. C.rlsberg, Sér. chim. 27, 307 (1950).
Hamberger, A.: Acta physiol. scand. 58, Suppl. 203 (1963).
Hamberger, A.: J. Neurochem. 8, 31 (1961).
Hamberger, A., and H. HydÉN: J. Cell Biol. 16, 521 (1963).
Holter, H.: C. R. Lab. C.rlsberg, Sér. chim. 24, 399 (1943).
Holter, H.: The Cartesian diver. In: General cytochemical methods, vol. 2, Ed. J. F. Danielli. pp. 93–129. New York and London: Academic Press 1961.
Holter, H., K. Linderstrom-Lang, and E. Zeuthen: Manometric techniques for single cells. In: Physical techniques in biological research, vol. 3, Eds. G. Oster and A. W. Pollister, pp. 557–625. New York: Academic Press 1956.
Holter, H., and B. A. LowY: C. R. Lab. Carlsberg 31, 105 (1959).
Holter, H., and S. Lovtrup: C. R. Lab. C.rlsberg, Sér. chim. 27, 27 (1949).
Holter, H., and M. Moller: Exp. Cell Res. 15, 631 (1958).
Holter, H., and B. M. Pollocx: C. R. Lab. C.rlsberg, Sér. chim. 28, 221 (1951).
Holter, H., and E. Zeuthen: C. R. Lab. C.rlsberg, Sér. chim. 26, 277 (1948).
Holter, H., and E. Zeuthen: Pubbl. Staz. Napoli 29, 285 (1957).
HydÉN, H.: Nature (Lond.) 184, 433 (1959).
HydÉN, H., and P. W. Lange: J. Cell Biol. 13, 233 (1962).
HydÉN, H., S. Lovtrup, and A. Pigon: J. Neurochem. 2, 304 (1958).
HydÉN, H., and A. Pigon: J. Neurochem. 6, 57 (1960).
Krugelis, E. J.: C. R. Lab. C.rlsberg, Sér. chim. 27, 273 (1950).
Linderstrom-Lang, K.: Nature (Lond.) 140, 108 (1937).
Linderstrom-Lang, K.: C. R. Lab. Carlsberg, Sér. chim. 24, 333 (1943).
Lovlie, A.: Kemisk Mânedsblad Nr 3, Copenhagen [in Norwegian language] (1961).
Lovlie, A.: C. R. Lab. Carlsberg 33, 377 (1963).
Lovlie, A.: C. R. Lab. Carlsberg. 34, 77 (1964).
Lovlie, A., and E. Zeuthen: C. R. Lab. Carlsberg 32, 513 (1962).
Lovtrup, S.: C. R. Lab. C.rlsberg, Sér. chim. 27, 125 (1950).
Lovtrup, S.: C. R. Lab. Carlsberg, Sér. chim. 27, 137 (1950).
Lovtrup, S., and A. Pigox: C. R. Lab. C.rlsberg, Sér. chim. 28, 1 (1951).
Lovtrup, S.: C. R. Lab. C.rlsberg, Sér. chim. 28, 371 (1953).
Lovtrup, S.: C. R. Lab. C.rlsberg, Sér. chim. 28, 400 (1953).
Lovtrup, S.: C. R. Lab. C.rlsberg, Sér. chim. 28, 426 (1953).
Lovtrup, S.: J. exp. Zool. 140, 231 (1959).
Lovtrup, S.: J. exp. Zool. 140, 383 (1959).
Lovtrup, S.: J. exp. Zool. 145, 139 (1960).
Lovtrup, S., and B. WernInius: J. exp. Zool. 135, 203 (1957).
Nigox, V., et J. Fourche: Bull. biol. Françe et Belg. 17, 36 (1958).
Pigox, A., u. E. Zeuthen: Experientia (Basel) 7, 455 (1951).
Prescott, D. M.: Exp. Cell Res. 9, 328 (1955).
Prescott, D. M.: Exp. Cell Res. 11, 86 (1956).
Prescott, D. M.: J. cell. comp. Physiol. 45, 1 (1955).
Prescott, D. M., and D. Mazia: Exp. Cell Res. 6, 177 (1954).
Prescott, D. M., and E. Zeuthen: Acta physiol. scand. 28, 77 (1953).
Satir, P., and E. Zeuthen: C. R. Lab. Carlsberg 32, 241 (1961).
Schwartz, S.: C. R. Lab. C.rlsberg, Sér. chim. 27, 79 (1949).
Waterlow, J. C., and A. Borrow: C. R. Lab. C.rlsberg, Sér. chim. 27, 93 (1949).
Zajicek, J.: Acta physiol. scand. 40, Suppl. 138 (1957).
Zajicek, J., and E. Zeuthen: Exp. Cell Res. 11, 568 (1957).
Zajicek, J., and E. Zeuthen: Quantitative determination by a special “Ampulla-Diver” of cholinesterase activity in individual cells, with notes on other uses of the method. In: General cytochemical methods II, edit. by J. F. Danielli, p. 131. New York and London: Academic Press 1961.
Zeuthen E.: C. R. Lab. C.rlsberg, Sér. chim. 25, 191 (1946).
Zeuthen E.: C. R., Lab. C.rlsberg, Sér. chim. 26, 243 (1948).
Zeuthen E.: C. R. Lab. C.rlsberg, Sér. chim. 26, 267 (1948).
Zeuthen E.: Biol. Bull. 98, 139 (1950).
Zeuthen E.: J. Embryol. exp. Morph. 1, 239 (1953).
Zeuthen E.: Biol. Bull. 108, 366 (1955).
Zeuthen E.: Exp. Cell Res. 19, 1 (1960).
Zeuthen E.: The Cartesian diver balance. In: General cytochemical methods II, edit. by J.F. Danielli, p. 61. New York and London: Academic Press 1961.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1964 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Seligman, A.M. et al. (1964). Referate. In: Schiebler, T.H., Pearse, A.G.E., Wolff, H.H. (eds) Zweiter Internationaler Kongreß für Histo- und Cytochemie / Second International Congress of Histo- and Cytochemistry / Deuxième Congrès International d’Histochimie et de Cytochimie. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-24616-0_2
Download citation
DOI: https://doi.org/10.1007/978-3-662-24616-0_2
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-662-22687-2
Online ISBN: 978-3-662-24616-0
eBook Packages: Springer Book Archive