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Embryo Culture of Wheat — Regenerative Tissue Culture System

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Wheat

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 13))

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Abstract

Embryo culture has often been applied to practical problems in plant breeding. For example, inviable embryos in vivo, resulting from unsuccessful crosses, can be grown in vitro in a nutrient medium. This topic has been frequently discussed (see Raghavan 1977; Norstog 1979). Young embryos of cereals can be cultured in vitro to obtain regenerative cultured tissues from which many roots and shoots can be initiated. Regenerative cultures have been obtained by utilizing immature embryos in maize (Green and Phillips 1975), oats (Cummings et al. 1976), sorghum (Gamborg et al. 1977; Thomas et al. 1977), barley (Dale and Deambrogio 1979), triticale (Sharma et al. 1980), and wheat (Shimada 1978; Gosch-Wackerle et al. 1979).

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References

  • Adachi T, Katayama Y (1989) Callus formation and shoot differentiation in wheat tissue culture. Bull Fac Agric Univ Miyazaki 16: 77–82

    Google Scholar 

  • Ahloowalia BS (1982) Plant regeneration from callus culture in wheat. Crop Sci 22: 405–410

    Article  Google Scholar 

  • Bajaj YPS (1986) In vitro regeneration of diverse plants and the cryopreservation ofgermplasm of wheat (Triticum aestivum L.) Cereal Res Commun 14: 305–311

    Google Scholar 

  • Cummings DP, Green CE, Stuthman DD (1976) Callus induction and plant regeneration in oats. Crop Sci 16: 465–470

    Article  Google Scholar 

  • Dale PJ, Deambrogio E (1979) A comparison of callus induction and plant regeneration from different explants of Hordeum vulgare. Z Pflanzenphysiol 94: 65–77

    CAS  Google Scholar 

  • Dudits D, Nemet G, Haydu Z (1975) Study of callus growth and organ formation in wheat (Triticum aestivum) tissue cultures. Can J Bot 53: 957–963

    Article  Google Scholar 

  • Dunstan DI, Short KC, Thomas E (1978) The anatomy of secondary morphogenesis in cultured scutellum tissues of Sorghum bicolor. Protoplasma 97: 251–260

    Article  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158

    Article  PubMed  CAS  Google Scholar 

  • Gamborg OL, Shyluck JP, Brar DS, Constabel F (1977) Morphogenesis and plant regeneration from callus of immature embryos of sorghum. Plant Sci Lett 10: 67–74

    Article  CAS  Google Scholar 

  • Gosch-Wackerle G, Avivi GL, Galun E (1979) Induction, culture and differentiation of callus from immature rachises, seeds and embryos of Triticum. Z Pflanzenphysiol 91: 267–278

    Google Scholar 

  • Green CE, Phillips RL (1975) Plant regeneration from tissue culture of maize. Crop Sci 15: 417–421

    Article  Google Scholar 

  • Heyser JW, Nabors MW, McKinnon C, Dykes TA, Demott KJ, Kautzman DC, Mujeeb-Kazi A (1985) Long-term, high-frequency plant regeneration and the induction of somatic embryogenesis in callus cultures of wheat (Triticum aestivum L.). Z Pflanzenzücht 94: 218–233

    Google Scholar 

  • Karp A, Maddock SE (1984) Chromosome variation in wheat plants regenerated from cultured immature embryos. Theor Appl Genet 67: 249–255

    Article  Google Scholar 

  • Lapitan NLV, Sears RG, Gill BS (1984) Translocations and other karyotypic structural changes in wheat X rye hybrids regenerated from tissue culture. Theor Appl Genet 68: 547–554

    Article  Google Scholar 

  • Larkin PJ, Scowcroft WR (1981) Somaclonal variation — a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60: 197–214

    Article  Google Scholar 

  • Larkin PJ, Ryan SA, Brettell RJS, Scowcroft WR (1984) Heritable somaclonal variation in wheat. Theor Appl Genet 67: 443–455

    Article  CAS  Google Scholar 

  • Lazar MD, Collins CB, Vian WE (1983) Genetic and environmental effects on the growth and differentiation of wheat somatic cell cultures. J Hered 74: 353–357

    Google Scholar 

  • Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18: 100–127

    Article  CAS  Google Scholar 

  • Lu C, Vasil IK, Ozias-Akins P (1982) Somatic embryogenesis in Zea mays L. Theor Appl Genet 62: 109–112

    Article  Google Scholar 

  • Maddock SE, Lancaster VA, Risiott R, Franklin J (1983) Plant regeneration from cultured immature embryos and inflorescences in 25 cultivars of wheat (Triticum aestivum). J Exp Bot 34: 915–926

    Article  Google Scholar 

  • Magnusson I, Bornman CH (1985) Anatomical observations on somatic embryogenesis from scutellar tissues of immature zygotic embryos of Triticum aestivum. Physiol Plant 63: 137–146

    Article  Google Scholar 

  • Maekawa KM (1981) Genetic studies on alloplasmic lines of three hexaploid synthetics in Triticinae. MS Thesis, Fac Agric Kyoto Univ

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Article  CAS  Google Scholar 

  • Nakamura T, Keller WA (1982) Callus proliferation and plant regeneration from immature embryos of hexaploid triticale. Z Pflanzenzücht 88: 137–160

    Google Scholar 

  • Norstog K (1979) Embryo culture as a tool in the study of comparative and developmental morphology. In: Sharp WR, Larsen PO, Paddock EF, Raghavan V (eds) Plant cell and tissue culture: principles and applications. Ohio State Univ Press, Columbus, pp 179–202

    Google Scholar 

  • Ogura H, Shimada T (1978) Preliminary report on plant redifferentiation from wheat callus. Wheat Inf Sery 45,46: 26–28

    Google Scholar 

  • O’Hara JF, Street HE (1978) Wheat callus culture: the initiation, growth and organogenesis of callus derived from various explant sources. Ann Bot (London) 42: 1029–1038

    Google Scholar 

  • Ozias-Akins P, Vasil IK (1982) Plant regeneration from cultured immature embryos and inflorescences of Triticum aestivum L. (wheat); evidence for somatic embryogenesis. Protoplasma 110: 95–105

    Article  Google Scholar 

  • Ozias-Akins P, Vasil IK (1983) Improved efficiency and normalization of somatic embryogenesis in Triticum aestivum (wheat). Protoplasma 117: 40–44

    Article  Google Scholar 

  • Raghavan V (1977) Applied aspects of embryo culture. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspect of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 375–397

    Google Scholar 

  • Sears RG, Deckard EL (1982) Tissue culture variability in wheat: callus induction and plant regeneration. Crop Sci 22: 546–550

    Article  Google Scholar 

  • Sharma GC, Belb LL, Sapra VT (1980) Genotypic differences in organogenesis from callus often triticale lines. Euphytica 29: 751–754

    Article  Google Scholar 

  • Shimada T (1978) Plant regeneration from the callus induced from wheat embryos. Jpn J Genet 53: 371–374

    Article  Google Scholar 

  • Shimada T, Yamada Y (1979) Wheat plants regenerated from embryo cell cultures. Jpn J Genet 54: 379–385

    Article  Google Scholar 

  • Shimada T, Sasakuma T, Tsunewaki K (1969) In vitro culture of wheat callus tissue. I. Callus formation, organ redifferentiation and single cell culture. Can J Genet Cytol 11: 294–304

    Google Scholar 

  • Shimada T, Koba K, Otani M, Niizeki H (1987) Morphology, meiosis and in vitro propagation of barley-wheat hybrids. In: Yasuda S, Konishi T (eds) Barley genetics V. 5th Int Barley Genetics Symp, Okayama

    Google Scholar 

  • Springer WD, Green CE (1979) A cytological examination of tissue culture initiation from immature embryos of maize. Protoplasma 101: 269–281

    Article  Google Scholar 

  • Thomas E, King P, Potrykus I (1977) Shoot and embryo-like structure formation from cultured tissues of Sorghum bicolor. Naturwissenshaften 64: 587

    Article  Google Scholar 

  • Trione EJ, Jones LE, and Metzger WI (1968) In vitro culture of somatic wheat callus tissue. Am J B of 55: 529–531

    Article  Google Scholar 

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© 1990 Springer-Verlag Berlin Heidelberg

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Shimada, T. (1990). Embryo Culture of Wheat — Regenerative Tissue Culture System. In: Bajaj, Y.P.S. (eds) Wheat. Biotechnology in Agriculture and Forestry, vol 13. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-10933-5_13

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  • DOI: https://doi.org/10.1007/978-3-662-10933-5_13

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-08081-4

  • Online ISBN: 978-3-662-10933-5

  • eBook Packages: Springer Book Archive

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