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Cellular Mortality and Immortalization: A Complex Interplay of Multiple Gene Functions

  • Chapter
Cell Immortalization

Part of the book series: Progress in Molecular and Subcellular Biology ((PMSB,volume 24))

Abstract

Since the pioneering work of Hayflick and Moorhead (1961) it has been generally accepted that normal somatic cells when cultured can undergo only a limited number (depending on the cell type) of divisions and reach an irreversibly growth arrested, but viable stage. The age of cells is determined by the number of times cells divide rather than the calendar time elapsed. The restricted replicative capacity of normal cells which confers them the mortal divisional phenotype is widely accepted as the most consistent manifestation of cellular aging. Relevance of in vitro life span of cells to in vivo aging is evidenced by

  1. (1)

    the correlation of in vitro life span and the donor age

  2. (2)

    correlation between in vitro life span with the average life expectancy of the species, and

  3. (3)

    the reduced life span of cells from patients afflicted with premature aging syndromes (Smith and Pereira-Smith 1996; Kaul et al. 1998a).

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Authors and Affiliations

  1. Chugai Research Institute for Molecular Medicine, 300-41, 153-2 Nagai,Ibaraki, Japan

    R. Wadhwa

  2. National Institute of Bioscience and Human-Technology, AIST, 1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan

    S. C. Kaul & Y. Mitsui

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  1. R. Wadhwa
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  2. S. C. Kaul
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  3. Y. Mitsui
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  1. INSERM, 73 bis, Rue du Meréchal Foch, 78000, Versailles, France

    Alvaro Macieira-Coelho

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Wadhwa, R., Kaul, S.C., Mitsui, Y. (1999). Cellular Mortality and Immortalization: A Complex Interplay of Multiple Gene Functions. In: Macieira-Coelho, A. (eds) Cell Immortalization. Progress in Molecular and Subcellular Biology, vol 24. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-06227-2_9

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  • DOI: https://doi.org/10.1007/978-3-662-06227-2_9

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