Abstract
An understanding of the protein adaptations that support mammalian hibernation is coming from several different approaches. New studies in my lab are (a) using cDNA library screening to identify genes that are up-regulated in hibernation, (b) analyzing the role of reversible protein phosphorylation in the control of membrane ion pumps in torpor, (c) assessing temperature-dependent properties of protein kinases that alter their function in euthermic vs hibernating states, and (d) characterizing fatty acid binding proteins of hibernating vs nonhibernating species to identify properties that support intracellular fatty acid transport at low body temperatures.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Andrews MT, Squire TL, Bowen CM, Rollins MB (1998) Low-temperature carbon utilization is regulated by novel gene activity in the heart of a hibernating mammal. Proc Natl Acad Sci. USA 95: 8392–8397
Banaszak L, Winter N, Xu Z, Bernlohr DS, Cowan W, Jones TA (1994) Lipid binding proteins. A family of fatty acid and retinoid transport proteins. Adv Protein Chem 45: 89–151
Barton PJ, Cohen A, Robert IB, Fiszman MY, Bonhomme F, Guenet JL, Leader DP, Buckingham ME (1985) The myosin alkali light chains of the mouse ventricular and slow skeletal muscle are indistinguishable and are encoded by the same gene. J Biol Chem 260: 8758–8584
Boyer BB, Barnes BM, Lowell BB, Grujic D (1998) Differential regulation of uncoupling protein gene homologues in multiple tissues of hibernating ground squirrels. Am J Physiol 275: R1232-R1238
Brooks SPJ, Storey KB (1992) Mechanisms of glycolytic control during hibernation in the ground squirrel Spermophilus lateralis. J Comp Physiol 162: 23–28
Cai Q, Storey KB (1996) Anoxia-induced gene expression in turtle heart: up-regulation of mitochondrial genes for NADH-ubiquinone oxidoreductase subunit 5 and cytochrome C oxidase subunit 1. Eur J Biochem 241: 83–92
Carey HV, Martin SL (1996) Preservation of intestinal expression during hibernation. Am J Physiol 271: G804-G813
Clausen T (1986) Regulation of active Na+K+ transport in muscle. Physiol Rev 66: 542–576
Dicker A, Cannon B, Nedergaard J (1996) Stimulation of non-shivering thermogenesis in the Syrian hamster by norepinephrine & ß-adrenergic agents. Comp Biochem Physiol C 113: 37–43
Fahlman A, Storey JM, Storey KB 2000. Gene up-regulation in heart during mammalian hibernation. Cryobiology in press
Frank CL, Storey KB (1995) The optimal depot fat composition for hibernation by goldenmantled ground squirrels (Spermophilus lateralis). J Comp Physiol B 164: 536–542
Frerichs KU, Smith CB, Brenner M, DeGracia DJ, Krause GS, Marrone L, Dever TE, Hallenbeck JM (1998) Suppression of protein synthesis in brain during hibernation involves inhibition of protein initiation and elongation. Proc Natl Acad Sci USA 95: 14511–14516
Gorham DA, Bretscher A, Carey HV (1998) Hibernation induces expression of moesin in intestinal epithelia cells. Cryobiology 37: 146–154
Holden CP, Storey KB (1998) Protein kinase A catalytic subunit from bat skeletal muscle: a kinetic study of the enzyme from a hibernating mammal. Arch Biochem Biophys 358: 243–250
Immaculada M, Octavi V, Mampel T, Iglesias R, Villarroya F (1993) Effects of cold environment on mitochondrial genome expression in the rat: evidence for a tissue-specific increase in the liver, independent of changes in mitochondrial abundance. Biochem J 296, 231–234
Kikkawa U, Kishimoto A, Nishizuka Y (1989) The protein kinase C family: heterogeneity and its implications. Annu Rev Biochem 58: 31–44
MacDonald JA, Storey KB (1998) cAMP-dependent protein kinase from brown adipose tissue: temperature effects on kinetic properties and enzyme role in hibernating ground squirrels. J Comp Physiol B 168: 513–525
MacDonald JA, Storey KB (1999) Regulation of ground squirrel Na+ K+-ATPase activity by reversible phosphorylation during hibernation. Biochem Biophys Res Commun 254: 424–429
Mehrani H, Storey KB (1997) Protein kinase C from bat brain: the enzyme from a hibernating mammal. Neurochem Int 31: 139–150
Morano I, Adler K, Agostini B, Hasselbach W (1992) Expression of myosin heavy and light chains and phosphorylation of the phosphorylatable myosin light chain in the heart ventricle of the European hamster during hibernation and in summer. J Muscle Res Cell Motil 13: 64–70
O’Hara BF, Watson FL, Srere HK, Kumar H, Wiler SW, Welch SK, Bitting L, Heller HC, Kilduff TS (1999) Gene expression in the brain across the hibernation cycle. J Neurosci 19: 3781–3790
Pehowich DJ (1994) Modification of skeletal muscle sarcoplasmic reticulum fatty acyl composition during arousal from hibernation. Comp Biochem Physiol B 109: 571–578
Richieri GV, Ogata RT, Kleinfeld AM (1995) Thermodynamics of fatty acid binding to fatty acid-binding proteins and fatty acid partition between water and membranes measured using the fluorescent probe ADIFAB. J Biol Chem 270: 15076–15084
Srere HK, Wang LCH, Martin SL (1992) Central role for differential gene expression in mammalian hibernation. Proc Natl Acad Sci USA 89: 7119–7123
Stewart JM, English TE, Storey KB (1998) Comparisons of the effects of temperature on the liver fatty acid binding proteins from hibernator and nonhibernator mammals. Biochem Cell Biol 76: 593–599
Storey KB (1997) Metabolic regulation in mammalian hibernation: enzyme and protein adaptations. Comp Biochem Physiol A 118: 1115–1124
Storey KB, Storey JM (1990) Facultative metabolic rate depression: molecular regulation and biochemical adaptation in anaerobiosis, hibernation and estivation. Quart Rev Biol 65: 145–174
Wada M, Pette D (1993) Relationships between alkali light-chain complement and myosin heavy-chain isoforms in single fast-twitch fibers of rat and rabbit. Eur J Biochem 214: 157–161
Wang LCH (1989) Ecological, physiological and biochemical aspects of torpor in mammals and birds. In: Wang LCH (ed) Advances in comparative and environmental physiology, vol 4. Springer, Heidelberg, pp 361–401
Wang LCH, Lee TF (1996) Torpor and hibernation in mammals: metabolic, physiological, and biochemical adaptations. In: Fregley MJ, Blatteis CM, (eds) Handbook of physiology: environmental physiology, section 4, vol 1. Oxford University Press, New York, pp. 507–532
Wickler SJ, Hoyt DF, van Breukelen F (1991) Disuse atrophy in the hibernating golden-mantled ground squirrel, Spermophilus lateralis. Am J Physiol 261: R1214–R1217
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Storey, K.B., Storey, J.M. (2000). Gene Expression and Protein Adaptations in Mammalian Hibernation. In: Heldmaier, G., Klingenspor, M. (eds) Life in the Cold. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-04162-8_33
Download citation
DOI: https://doi.org/10.1007/978-3-662-04162-8_33
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-08682-3
Online ISBN: 978-3-662-04162-8
eBook Packages: Springer Book Archive