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Embryonic Organs in Culture

  • Chapter
Teratogenesis and Reproductive Toxicology

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 65))

Abstract

It is not my purpose to review the field of experimental embryology that deals with tissue culture of mammalian embryonic organs (Wessells 1967). A number of embryonic organs and tissues are known to undergo morphogenesis and attain functional maturation upon explanation in vitro, and their capabilities have been exploited to a variable degree in investigations on developmental mechanisms and the processes of cell differentiation (Saxén and Kohonen 1969). Some examples are: kidney (Grobstein 1956; Saxén et al. 1968), sex organs (Odor and Blandau 1971; Haffen 1976), pancreas (Sarras et al. 1981; Maylde-Pfenninger and Jamieson 1981; Grobstein 1953), skin (Sugimoto and Endo 1969), tooth (Thesleff 1976; Thesleff et al. 1981), palate (Moriarty et al. 1963; Lahti et al. 1972; Newall and Edwards 1976, 1981; Greene and Pratt 1977), other craniofacial tissues (Johnston and Listgarten 1972; Johnston et al. 1977; Greenberg and Schrier 1977), and limbs (Agnish and Kochhar 1977; Kochhar et al. 1976). Any of these or other developing organs are suitable subjects for the development of a teratologic screening system. This chapter, however, is limited to just one example, i.e., limb bud, that may suffice to bring out feasibility, advantages, and shortcomings of organ culture system in general for short-term teratologic screening purposes.

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Authors
  1. D. M. Kochhar
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Editors and Affiliations

  1. Jefferson Medical College, 1020 Locust Street, Philadelphia, PA, 19107, USA

    E. Marshall Johnson Ph.D. (Professor and Chairman of Department of Anatomy, Director of Daniel Baugh Institute) (Professor and Chairman of Department of Anatomy, Director of Daniel Baugh Institute)

  2. Department of Anatomy, Daniel Baugh Institute, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA, 19107, USA

    Devendra M. Kochhar Ph.D.

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© 1983 Springer-Verlag Berlin Heidelberg

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Kochhar, D.M. (1983). Embryonic Organs in Culture. In: Johnson, E.M., Kochhar, D.M. (eds) Teratogenesis and Reproductive Toxicology. Handbook of Experimental Pharmacology, vol 65. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-81919-3_15

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  • DOI: https://doi.org/10.1007/978-3-642-81919-3_15

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-81921-6

  • Online ISBN: 978-3-642-81919-3

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