Abstract
A vitamin D-dependent calcium binding protein (CaBP) has been partially purified from rat renal cortex. Its molecular weight is about 28.000 daltons, more than twice that of the vitamin D-dependent rat intestinal CaBP. The apparent pK of the partially purified renal CaBP is 5.0 and its binding capacity 22 nanomoles Ca bound/mg protein. No renal CaBP was found in kidneys from rachitic rats or in the medulla of normal animals. In contrast with intestinal CaBP, renal CaBP activity does not appear to vary markedly with calcium intake, plasma calcium concentration or urinary calcium excretion.
We thank KUKIL and BURNS for technical assistance. Supported by NIH grants AM 14251 and DE 02593.
A complete report has appeared in Biochim. Biophys. Acta (in press).
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Hermsdorf, C.L., Freund, T., Bronner, F. (1975). Vitamin D-Dependent Calcium Binding Protein of Rat Renal Cortex. In: Kuhlencordt, F., Kruse, HP. (eds) Calcium Metabolism, Bone and Metabolic Bone Diseases. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80875-3_10
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DOI: https://doi.org/10.1007/978-3-642-80875-3_10
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