Abstract
The easiest method to study involvement of individual classes of sugar receptors such as membrane lectins and their carbohydrate ligands in cellular interactions in a model is established by deliberately reducing the intriguing complexity of surface receptors or ligands, resulting in surrogate systems. Guided by this framework, liposomes are prepared whose surface can be aimingly modified by chemical conjugation of potential receptors or ligands to be tested. Their interaction with respective ligand- and receptor-exposing surfaces, liposomes or cells helps to infer the generated strength of this type of receptor-ligand recognition. Moreover, these devices may be helpful for cell type-specific targeting. This chapter outlines the preparation, characterization, and selected application of neoglycoprotein-liposome conjugates which are instrumental in analysis of cell surface lectins.
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References
Gabius S, Hellmann K-P, Hellmann T, Brinck U, Gabius H-J (1989) Neoglycoenzymes: a versatile tool for lectin detection in solid-phase assays and glycohistochemistry. Anal Biochem 182: 447–451
Heath TD, Macher BA, Papahadjopoulos D (1981) Covalent attachment of immunoglobulins to liposomes via glycosphingolipids. Biochim Biophys Acta 640: 66–81
Kojima S, Gabius H-J (1988) Biodistribution of neoglycoproteins in mice bearing solid Ehrlich tumor. J Cancer Res Clin Oncol 114: 468–472
Markwell MAK, Haas SH, Bieber LL, Tolbert NE (1978) A modification of the Lowry procedure to simplify protein determination on membrane and lipoprotein samples. Anal Biochem 87: 206–210
Mroom CR, Samanen CH, Goldstein IJ (1972) Carbohydrate antigens: coupling of carbohydrates to proteins by diazonium and phenylisothiocyanate. Meth Enzymol 28: 212–219
Yamazaki N (1987) Multivalent interaction between polysaccharide fragments and lectin-conjugated lipid vesicles: an affinity system to probe the structural features of carbohydrates. In: Zlatkis A (ed) Advances in chromatography 1986, Part II. Elsevier, Amsterdam, pp 371–380
Yamazaki N (1989) Analysis of the carbohydrate-binding specificity of lectin-conjugated lipid vesicles which interact with polysaccharide fragments. J Membr Sci 41: 249–267
Yamazaki N, Kojima S, Gabius S, Gabius H-J (1992) Studies on carbohydrate-binding proteins using liposome-based systems. I. Preparation of neoglycoprotein-conjugated liposomes and the feasibility of their use as drug-targeting devices. Int J Biochem 24;99–104
Zumbuehl O, Weder HG (1981) Liposomes of controllable size in the range of 40 to 180 nm by defined dialysis of lipid/detergent mixed micelles. Biochim Biophys Acta 640:252–262
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© 1993 Springer-Verlag Berlin Heidelberg
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Yamazaki, N., Gabius, S., Kojima, S., Gabius, HJ. (1993). Neoglycoprotein-Liposome Conjugates for Studies of Membrane Lectins. In: Gabius, HJ., Gabius, S. (eds) Lectins and Glycobiology. Springer Laboratory. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77944-2_33
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DOI: https://doi.org/10.1007/978-3-642-77944-2_33
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-77946-6
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