Abstract
The S-type (thiol-dependent) or S-Lac (soluble lactose-binding) β-galactoside-specific lectins are found in a wide variety of tissue and cells (Caron et al. 1990). Human brain contains a lectin of this family that has been isolated (Bladier et al. 1989) and subsequently has been well characterized (Bladier et al. 1991; Joubert et al. 1992). The subunit molecular weight (14.5 K) and amino acid composition of the human brain lectin (HBL) were similar to those for human placenta lectin (Hirabayashi and Kasai 1988).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Avellana-Adalid V, Joubert R, Bladier D, Caron M (1990) Biotinylated derivative of a human brain lectin: synthesis and use in affinoblotting for endogenous ligand studies. Anal Biochem 190: 26–31
Bladier D, Joubert R, Avellana-Adalid V, Kemeny JL, Doinel C, Amouroux J, Caron M (1989) Purification and characterization of a galactoside-binding lectin from human brain. Arch Biochem Biophys 269: 433–439
Bladier D, Le Caer JP, Joubert R, Caron M, Rossier J (1991) β-Galactoside soluble lectin from human brain. Complete amino acid sequence. Neurochem Int 18: 275–281
Caron M, Bladier D, Joubert R (1990) Soluble galactoside-binding vertebrate lectins: a protein family with common properties. Int J. Biochem 22: 1379–1385
Eloumami H, Caron M, Joubert R, Doinel C, Bladier D (1991) Human brain lectin immunoreactive material in cerebrospinal fluids determined by enzyme immunoassay (EIA). J Neurol Sci 105: 6–11
Hirabayashi J, Kasai Kl (1988) Complete amino-acid sequence of a β-galactoside-binding lectin from human placenta. J Biochem 104: 1–4
Joubert R, Caron M, Avellana-Adalid V, Mornet D, Bladier D (1992) Human brain lectin: a soluble lectin that binds actin. J Neurochem 58: 200–203
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Caron, M. (1993). An Enzyme Immunoassay (EIA) for Human Endogenous β-Galactoside-Binding Lectin. In: Gabius, HJ., Gabius, S. (eds) Lectins and Glycobiology. Springer Laboratory. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77944-2_19
Download citation
DOI: https://doi.org/10.1007/978-3-642-77944-2_19
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-77946-6
Online ISBN: 978-3-642-77944-2
eBook Packages: Springer Book Archive