Abstract
Formation of suppressor T cells (Ts) induced by donor-specific transfusion (DST) is one of the most commonly suggested mechanisms for the beneficial effect of DST. In this study, we established a human T cell hybridoma derived from the peripheral blood lymphocytes (PBL) of a DST-treated patient, which produced an antigen-nonspecific suppressor factor. Post-DST PBL were fused with an azaguanine-resistant mutant of a human T cell leukemia cell line, CCRF-CEMAG. After selection and cloning, we established one clone producing the mixed lymphocyte reaction (MLR) inhibitory factor (C524:18% -43% suppression). Suppressive activity of the supernatant obtained from C524 after activation by PHA was highly augmented (64%-88% MLR suppression). This factor inhibited MLR dose-dependently in an antigen-nonspecific and HLA non-restricted manner. These results indicated that Ts clones could be generated in patients receiving DST and that the immunoregulatory factors produced by activated clones may play a role in the prolongation of renal allograft survival.
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References
Emara M, Sanfilippo F (1989) Human suppressor T cells induced in vitro with an autologous renal allograft-derived T cell line II. activity and specificity of a soluble suppressor factor. Transplantation 47:364–371
Fischer A, Durandy A, Griscelli C (1981) Role of prostaglandin E2 in the induction of nonspecific T lymphocyte suppressor activity. J Immunol 126:1452–1455
Gralier AP, Sollinger HW, Kawamura T, Burlingham WJ (1991) Donor-specific cytotoxic lymphocyte hyporesponsiveness following renal transplantation in patients pretreated with donor-specific transfusion. Transplantation 51:320–324
Haisa M, Sakagami K, Matsumoto T, Kawamura T, Uchida S, Fujiware T, Shiozaki S, Inagaki M, Orita K (1989) Donor-specific transfusion (DST) with intermittent administration of azathio-prine induces suppressor T cells and MLR-inhibiting factors without sensitization. Transplant Proc 21:1814–1817
Haisa M, Sakagami K, Orita K (1990) Establishment of a human T-cell hybridoma producing an MLR suppressing factor: relationship to the mechanism of action of donor-specific transfusion. Transplant Proc 22:1977–1980
Kawamura T, Sakagami K, Haisa M, Morisaki F, Takasu S, Ina-gaki M, Oiwa T, Orita K (1989) Induction of antiidiotypic antibodies by donor-specific blood transfusions: establishment of a human-mouse hybridoma sectreting the MLR-inhibiting factor. Transplantation 48:459–463
Maki T, Satomi S, Gotoh M, Monaco AP (1986) Contra-IL2: a suppressor lymphokine that inhibits IL-2 activity. J Immunol 136: 3298–3303
Matsumoto T, Sakagami K, Orita K (1987) Role of donor specific blood transfusions in prolongation of kidney graft survival. Transplant Proc 19:2264–2267
Mohagheghpour N, Benike CJ, Kansas G, Bieber C, Engleman EG (1983) Activation of antigen-specific suppressor T cells in the presence of cyclosporin requires interactions between T cells of inducer and suppressor lineage. J Clin Invest 72:2092–2100
Okada M, Yoshimura N, Kaieda T, Yamamura Y, Kishimoto T (1981) Establishment and characterization of human T hybrid cells secreting immunoregulatory molecules. Proc Natl Acad Sci USA 78:7717–7721
Salvatierra O, Vincent F, Amend W, Potter D, Iwaki Y, Opelz G, Terasaki P, Duca R, Cochrum K, Hanes D, Stoney RJ, Feduska NJ (1980) Deliberate donor-specific blood transfusion prior to living related renal transplantation: a new approach. Ann Surg 192:543–552
Takeuchi T, Sakagami K, Seki Y, Tsuboi K, Matsumoto M, Miyazaki M, Orita K (1985) Antiidiotypic antibodies and suppressor cells induced by donor-specific transfusion in potential kidney transplant recipients. Transplant Proc 17:1059–1061
Walker C, Kristensen F, Bettens F, De Week AL (1983) Lymphokine regulation of activated (Gi) lymphocytes 1. prostaglandin E2-induced inhibition of interleukin 2 production. J Immunol 130:1770–1773
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© 1992 Springer-Verlag Berlin Heidelberg
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Fujiwara, T., Sakagami, K., Kusaka, S., Uda, M., Orita, K. (1992). Analysis of suppressor T cells induced by donor-specific transfusion (DST): establishment of a human T cell hybridoma producing an antigen-nonspecific suppressor factor. In: Kootstra, G., Opelz, G., Buurman, W.A., van Hooff, J.P., MacMaster, P., Wallwork, J. (eds) Transplant International Official Journal of the European Society for Organ Transplantation. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77423-2_192
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DOI: https://doi.org/10.1007/978-3-642-77423-2_192
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