Abstract
Severe microcirculatory disturbances due to endothelial cell damage and leukocyte adherence during reperfusion of transplanted livers are considered to contribute to early graft failure. Since the degree of reperfusion injury after liver transplantation depends on the length of preservation time and the solution used for preservation, the aim of our study was to assess three solutions with respect to microvascular perfusion and leukocyte adhesion. Therefore, rat livers were stored up to 24 h in Euro-Collins (EC), University of Wisconsin (UW), or histidin-tryphtophan-ketoglutarate (HTK) solutions prior to orthotopic transplantation. The livers were studied in situ 60 min postoperatively using intravital fluorescence video microscopy. Using simple syringe flushing (10 ml), sinusoidal perfusion decreased below 50% in EC preserved livers after 8 h preservation, in HTK preserved livers after 16 h preservation, and remained higher than 70% in livers preserved in UW up to 24 h. Permanent adhesion of leukocytes was increased more rapidly in organs after 1, 8, 16, and 24 h preservation in HTK (16%, 15%, 34%, and 49.7% ± 4.7%) compared to those preserved in UW (15%, 18%, 17%; and 32.7% ±3.3%; P < 0.05). Using a 10-fold volumn of the organ weight of HTK solution during the harvesting procedure, with an 8 min equilibration period, sinusoidal perfusion (39.6 ±4.7%) and leukocyte adhesion (42.7 ±3.1%) were not improved after 24 h. In contrast, equilibration with a volumn of approximately 40-times the liver weight improved sinusoidal perfusion (70.8% ±2.7%; P < 0.01) and leukocyte adhesion (24.9% ±3.1%; P < 0.01) significantly. Thus, using HTK solution, simple flushing prior to long-term cold storage resulted in microcirculatory disturbances when compared to UW solution. Larger volumns of HTK solution with an additional equilibration period of 8 min, however, reduced leukocyte adhesion and improved sinusoidal perfusion to a similar degree as UW solution.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Belzer FO, Southard JH (1988) Principles of solid-organ preservation by cold storage. Transplantation 45:673–676
Bretschneider HJ, Helmchen U, Kehrer G (1988) Nierenprotektion. Klin Wochenschr 66:817–827
Caldwell-Kenkel JC, Thurman RG, Lemasters JJ (1988) Selective loss of nonparenchymal cell viability after cold ischemic storage of rat livers. Transplantation 45:834–837
Gubernatis G, Pichlmayr R, Lamesch P, Grosse H, Bornscheuer A, Meyer H-J, Ringe B, Farle M, Bretschneider HJ (1990) HTK-solution (Bretschneider) for human liver transplantation. Langenbecks Arch Chir 375:66–70
Kallerhoff M (1989) Nierenprotektion in Anlehnung an das Verfahren zur Myokardprotektion nach Bretschneider im Vergleich zum Euro-Collins Verfahren. Z Transplantationsmedizin 1:15–33
Kamada N, Calne RY (1979) Orthotopic liver transplantation in the rat. Technique using cuff for portal vein anastomosis and biliary drainage. Transplantation 28:47–50
Kubes P, Suzuki M, Granger DN (1990) Platelet-activating factor-induced microvascular dysfunction: Role of adherent leukocytes. Am J Physiol 258:G158–G163
Lawrence MB, Springer TA (1991) Leukocytes roll on a selectin at physiologic flow rates: Distinction from and prerequisite for adhesion through integrins. Cell 65:859–873
Marzi I, Takei Y, Knee J, Menger MD, Gores GJ, Bhren V, Trentz O, Lemasters JJ, Thurman RG (1990) Assessment of reperfusion injury by intravital fluorescence microscopy following liver transplantation in the rat. Transplant Proc 22:2004–2005
Marzi I, Walcher F, Bhren V, Menger MD, Knee J, Trentz O (1991) Microcirculatory disturbances and leucocyte adherence in transplanted livers after cold storage in Euro-Collins, UW and HTK solutions. Transplant Int 4:45–50
Marzi I, Zhong Z, Lemasters JJ, Thurman RG (1989) Evidence that graft survival is not related to parenchymal cell viability in rat liver transplantation: The importance of nonparenchymal cells. Transplantation 48:463–468
Momii S, Koga A, Eguchi M, Fukuyama T (1989) Ultrastructural changes in rat liver sinusoids during storage in cold Euro-Collins solution. Virchows Arch [B] 57:393–398
Olthoff KM, Millis JM, Imagawa DK, Nuesse BJ, Derus LJ, Rosenthal JT, Milewicz AL, Busuttil RW (1990) Comparison of UW solution and Euro-Collins solutions for cold preservation of human liver grafts. Transplantation 49:284–290
Pober JS, Cotran RS (1990) The role of endothelial cells in inflammation. Transplantation 50:537–544
Southard JH, van Gulik TM, Ametani MS, Vreugdenhil PK, Lindell SL, Pienaar BL, Belzer FO (1990) Important components of the UW solution. Transplantation 49:251–257
Sumimoto R, Kamada N, Jamieson NV, Fukuda Y, Dohi K (1991) A comparison of a new solution combining histidine and lactobionate with UW solution and Eurocollins for rat liver preservation. Transplantation 51:589–593
Takaoka F, Brown MR, Ramsay MAE, Paulsen AW, Brajtbord D, Klintmalm GB (1990) Intraoperative evaluation of EuroCollins and University of Wisconsin preservation solutions in patients undergoing hepatic transplantation. Transplantation 49:544–547
Takei Y, Marzi I, Kauffman FC, Lemasters JJ, Thurman RG (1990) Increase in survival time of liver transplants from injury by protease inhibitors and a calcium channel blocker, nisoldipine. Transplantation 50:14–20
Thurman RG, Marzi I, Seitz G, Thies J, Lemasters JJ, Zimmermann FA (1988) Hepatic reperfusion injury following orthotopic liver transplantation in the rat. Transplantation 46:502–506
Wake K, Decker K, Kirn A, Knook DL, McCuskey RS, Bouwens L, Wisse E (1989) Cell biology and kinetics of Kupffer cells in the liver. Int Rev Cytol 118:173–230
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Walcher, E., Marzi, I., Bühren, V. (1992). The impact of liver preservation in HTK and UW solution on microcirculation after liver transplantation. In: Kootstra, G., Opelz, G., Buurman, W.A., van Hooff, J.P., MacMaster, P., Wallwork, J. (eds) Transplant International Official Journal of the European Society for Organ Transplantation. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77423-2_104
Download citation
DOI: https://doi.org/10.1007/978-3-642-77423-2_104
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-55342-7
Online ISBN: 978-3-642-77423-2
eBook Packages: Springer Book Archive