Abstract
Thyroid microsomal antibodies, recently identified as anti-thyroid peroxidase (anti-TPO) antibodies [1], are frequently present in patients with autoimmune thyroid disease and have been thought to be implicated in thyroid cell damage observed in autoimmune thyroiditis. In support of this concept, antibodies that are cytotoxic for thyroid cells in vitro have been found in the sera of patients with autoimmune thyroiditis and exhibiting antimicrosomal antibodies. These cytotoxic antibodies are active both directly in the presence of complement [2, 3, 4] and in association with K cells [5].
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Czarnocka B, Ruf J, Ferrand M, Carayon P, Lissitzky S (1985) Purification of the human thyroid peroxidase and its identification as the microsomal antigen involved in autoimmune thyroid diseases. FEBS Lett 190:147–152
Pulvertaft RJ, Doniach D, Roitt IM (1961) The cytotoxic factor in Hashimoto’s disease and its incidence in other diseases. Br J Exp Pathol 42:496–503
Forbes IJ, Roitt IM, Doniach D, Solomon IL (1962) The thyroid cytotoxic autoantibody. J Clin Invest 41:996–1006
Khoury EL, Hammond L, Bottazzo GF, Doniach D (1981) Presence of the organ specific “microsomal” autoantigen in the surface of human thyroid cells in culture: its involvement in complement-mediated cytotoxicity. Clin Exp Immunol 45:316–328
Schleusener H, Bogner U (1985) Cytotoxic mechanisms in autoimmune thyroiditis. In: Walfish PG, Wall JR, Volpe R (eds) Autoimmunity and the thyroid. Academic, pp 95–107
Ruf J, Czarnocka B, Ferrand M, Doullais F, Carayon P (1988) Novel routine assay of thy-roperoxidase autoantibodies. Clin Chem 34:2231–2234.
Wadeleux P, Winand-Devigne J, Ruf J, Carayon P, Winand R (1989) Cytotoxic assay of circulating thyroid peroxidase antibodies. Autoimmunity: 4:247–254
Garland JT, Lottes ME, Kozak S, Daughaday WH (1972) Stimulation of DNA synthesis in isolated chondrocytes by sulfation factor. Endocrinology 90:1086–1090
Bidey SP, Marshall NJ, Ekins RP (1980) Inhibition by normal immunoglobulins of thyro-tropin-stimulated production of cyclic AMP in slices of normal human thyroid. J Endocrinol 87:271–277
Labarca C, Paigen K (1980) A simple, rapid and sensitive DNA assay. Procedure. Anal Procedure 102:344–352
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal Biochem 72: 248–254
Kremers P, Goujon F, De Graeve J, Van Cantfort S, Gielen J (1981) Multiplicity of cytochrome P 450 in primary fetal hepatocytes in culture. Eur J Biochem 116:67–72
Joustra MJ, Lundgren H (1969) Preparation of freeze-dried monomeric and immuno-chemically pure IgG by a rapid and reproducible chromatographic technique. In: Peters H (ed) Protides of biological fluids. Proceedings of the 17th colloquium, Bruges, Belgium. Pergamon, Oxford, p511
Kunkel HG (1982) The immunoglobulins. In: Lackmann PJ, Peter PK (eds) Clinical aspects of immunology. Blackwell, Oxford, pp3–17
Dean BM, Bottazzo GF, Cudworth AG (1983) IgG subclass distribution in organ specific autoantibodies. The relationship to complement fixing ability. Clin Exp Immunol 52:61–66
McLachlan SM, Feldt-Rasmussen U, Young ET, Middleton SL, Blichert-Toft M, Siers-boek-Nielsen K, Date J, Carr D, Clark F, Rees Smith B (1987) IgG subclass distribution of thyroid autoantibodies: a “fingerprint” of an individual’s response to thyroglobulin and thyroid microsomal antigen. Clin Endocrinol 26:335–346
Kotani T, Kato E, Hirai K, Kuma K, Ohtaki S (1986) Immunoglobulin G subclasses of anti-thyroid peroxidase autoantibodies in human autoimmune thyroid diseases. Endocrinol Jpn 33:505–510
Jansson R, Thompson PM, Clark F, McLachlan SM (1986) Association between thyroid microsomal antibodies of subclass IgG-1 and hypothyroidism in autoimmune postpartum thyroiditis. Clin Exp Immunol 63:80–86
Bogner U, Schleusener H (1988) Cytotoxic and microsomal antibodies are not identical in Hashimoto’s thyroiditis. Abstract 82, 17th annual meeting of the European Thyroid Association, Montpellier, Sept, 11–16, 1988
Hiromatsu Y, Fukazawa H, Guinard F, Salvi M, How J, Wall JR (1988) A thyroid cytotoxic antibody that cross-reacts with an eye muscle cell surface antigen may be the cause of thyroid-associated ophthalmopathy. J Clin Endocrinol Metab 67:565–570
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Wadeleux, P., Ruf, J., Carayon, P., Winand, R. (1991). Comparison of Circulating Thyroid Microsomal, Cytotoxic and Thyroid Peroxidase Antibodies. In: Scherbaum, W.A., Bogner, U., Weinheimer, B., Bottazzo, G.F. (eds) Autoimmune Thyroiditis. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76301-4_16
Download citation
DOI: https://doi.org/10.1007/978-3-642-76301-4_16
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-53476-1
Online ISBN: 978-3-642-76301-4
eBook Packages: Springer Book Archive