Abstract
The precise mechanism of copper-induced hemolysis is not completely understood. Several hypotheses have been proposed. One states that copper ions (Cu II) may induce lipoperoxidation because of the occurrence of reactive oxygen species (Kumar et al. 1978; Barnes and Frieden 1983). These results were obtained from erythrocyte membranes or washed red cells. However, it is well known that plasma exerts an indubitable protective effect against copper-induced damage (Hiroshige 1980). So, in our study, lipoperoxidation was investigated in plasma and in whole blood incubated with different concentrations of copper for 24 h. In order to evaluate lipoperoxidation, thiobarbituric acid-reactive species (TBARS) and fluorescent chromolipids were assayed; polyunsaturated fatty acid (PUFA) profiles were studied. Recently Piriou et al. (1987) demonstrated that under air atmosphere, no lipoperoxidation occurred in red blood cells, whereas this phenomenon was evident in plasma in the presence of elevated concentrations of copper.
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References
Barnes G, Frieden E (1983) Oxygen requirement for cupric ion induced hemolysis. Biochem iophys Res Commun 115:680–684
Bidlack WR, Tappel AL (1973) Fluorescent products of phospholipids during lipid peroxidation. Lipids 8:203–207
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917
Chan PC, Peller OG, Kesner L (1982) Copper (II) — catalyzed lipid peroxidation in liposomes and erythrocyte membranes. Lipids 17:331–337
Hiroshige Y (1980) The effects of copper and copper-o-phenanthroline complex on the intact human erythrocytes. Tohoku J Exp Med 130:385–402
Hochstein P, Kumar KS, Forman SJ (1980) Lipid peroxidation and the cytotoxicity of copper. Ann NY Acad Sci 355:240–248
Kumar KS, Rowse C, Hochstein P (1978) Copper-induced generation of superoxide in red cell membrane. Biochem Biophys Res Commun 83:587–592
Piriou A, Tallineau C, Chahboun S, Pontcharraud R, Guillard O (1987) Copper-induced lipid peroxidation and hemolysis in whole blood: evidence for a lack of correlation. Toxicology 47:351–361
Yagi K (1976) A simple fluorometric assay for lipoperoxide in blood plasma. Biochem Med 15:212–216
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© 1988 Springer-Verlag
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Tallineau, C., Chahboun, S., Perault, M.C., Reiss, D., Piriou, A. (1988). Is the Noxious Hemolytic Effect of Copper a Result of Lipoperoxidation?. In: Chambers, P.L., Chambers, C.M., Dirheimer, G. (eds) The Target Organ and the Toxic Process. Archives of Toxicology, vol 12. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73113-6_86
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DOI: https://doi.org/10.1007/978-3-642-73113-6_86
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-18512-3
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