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Sympathetic modulation of the cardiac myocyte phenotype: studies with a cell-culture model of myocardial hypertrophy

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Cardiac Adaptation in Heart Failure

Summary

Myocardial hypertrophy is the common endpoint of many cardiovascular stimuli such as hypertension, myocardial infarction, valvular disease, and congestive failure. Catecholamines have long been implicated in the pathogenesis of myocardial hypertrophy, however, it is very difficult to sort out catecholamine mechanisms in vivo. We have developed a cell-culture model which excludes hemodynamic effects and allows the assignment of receptor specificity to catecholamine effects. Utilizing this system, we have shown that stimulation of the α1 adrenergic receptor leads to the development of myocardial hypertrophy and results in the selective up-regulation of the fetal/neonatal mRNAs encoding skeletal α-actin and β-MHC, a pattern similar to that seen with hypertrophy in-vivo. Utilizing a co-transfection assay, we have also obtained data that suggest that the β-PKC isozyme is in a pathway regulating transcription of the β-MHC isogene. β adrenergic stimulation of the cultured cardiac myocytes also results in a modest degree of hypertrophy, however, this effect may be dependent upon myocyte contractile activity and may involve, at least in part, the non-muscle cells present in the culture system.

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Author information

Authors and Affiliations

  1. Cardiology Section and Research Service, Veterans Administration Medical Center, San Francisco, California, USA

    C. S. Long M.D. (Assistant Professor of Medicine), K. Kariya, L. Karns & P. C. Simpson

  2. Cardiovascular Research Institute and Department of Medicine, University of California, San Francisco, USA

    C. S. Long M.D. (Assistant Professor of Medicine), K. Kariya, L. Karns & P. C. Simpson

  3. Div. of Cardiology 111-C8, Veterans Administration Medical Center, 4150 Clement St., San Francisco, CA, 94121, USA

    C. S. Long M.D. (Assistant Professor of Medicine)

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  1. C. S. Long M.D.
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  2. K. Kariya
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  3. L. Karns
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  4. P. C. Simpson
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Editors and Affiliations

  1. Halle-Wittenberg Medizinische Fakultät Institut fü Pathophysiologie, Martin-Luther-Universität, Magdeburger Str. 6, 0-4020, Halle/Saale, Germany

    J. Holtz

  2. Medizinische Universitätsklinik Innere Medizin III, Kardiologie, Albert-Ludwigs-Universität, Hugstetter Straße 55, W-7800, Freiburg, Germany

    H. Drexler  & H. Just  & 

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© 1992 Dr. Dietrich Steinkopff Verlag GmbH & Co. KG, Darmstadt

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Long, C.S., Kariya, K., Karns, L., Simpson, P.C. (1992). Sympathetic modulation of the cardiac myocyte phenotype: studies with a cell-culture model of myocardial hypertrophy. In: Holtz, J., Drexler, H., Just, H. (eds) Cardiac Adaptation in Heart Failure. Steinkopff. https://doi.org/10.1007/978-3-642-72477-0_3

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  • DOI: https://doi.org/10.1007/978-3-642-72477-0_3

  • Publisher Name: Steinkopff

  • Print ISBN: 978-3-642-72479-4

  • Online ISBN: 978-3-642-72477-0

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