Abstract
Similar to dot-blot immunoassay for detection of proteins (Subprotocol 41.2), dot-blot hybridisation is a useful technique for detection of nucleic acids when large numbers of samples have to be tested, as it can be carried out with crude leaf samples.
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References
Baulcombe DC, Flavell RB, Boulton RE, Jellis GJ (1984) The sensitivity and specificity of a rapid nucleic acid hybridisation method for the detection of potato virus X in crude sap samples. Plant Pathol 33: 361–370
Salazar LF, Querci M (1992) Detection of viroids and viruses by nucleic acid probes. In: Duncan JM, Torrance L (eds) Techniques for the rapid detection of plant pathogens. Blackwell Scientific, Cambridge, pp 129–144
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© 1998 Springer-Verlag Berlin Heidelberg
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Dijkstra, J., de Jager, C.P. (1998). Dot-Blot Hybridisation (Nucleic Acid Spot Hybridisation). In: Practical Plant Virology. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72030-7_63
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DOI: https://doi.org/10.1007/978-3-642-72030-7_63
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-48981-5
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