Abstract
In this protocol, a standard procedure is presented for isolation of meristems from apical buds. Success in meristem-tip culture depends on a large number of factors and is hard to predict. A procedure which has yielded good results with one plant species, or even one cultivar, may be unsuitable for another. It has been found, for example, that standard carnation and spray carnation require different culture media. Therefore, it is advised to first consult the literature to find out whether the species or cultivar under investigation has already been grown in vitro. If so, the procedure described should be followed. In case there are no data available, then try the procedure given below.
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© 1998 Springer-Verlag Berlin Heidelberg
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Dijkstra, J., de Jager, C.P. (1998). Meristem-Tip Culture. In: Practical Plant Virology. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72030-7_41
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DOI: https://doi.org/10.1007/978-3-642-72030-7_41
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-48981-5
Online ISBN: 978-3-642-72030-7
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