Skip to main content
SpringerLink
Log in

Mechanism for Elimination of Senescent Red Blood Cells from Circulation

  • Conference paper
Blood Cells, Rheology, and Aging

Abstract

Circulating normal human red blood cells (RBCs) have a life span of approximately 120 days in vivo (Berlin et al. 1959), after which they are sequestered within the confines of the reticuloendothelial system (Ashwell and Morell 1974) and then removed from circulation by mononuclear phagocytic cells (Gemsa et al. 1972; Kay 1975). The aging process in human erythrocytes correlates well with increasing RBC density; hence, this parameter is often used in evaluating preparations of erythrocyte subpopulations of different relative ages (Primella et al. 1973; Murphy 1973). During aging of these cells, a number of cryptic membrane glycoprotein antigens are exposed due to the loss of the terminal sialic acid residues from the major membrane sialoglycoproteins (Alderman et al. 1980). The exposure of these antigens leads to specific binding of serum autoantibody (Hunt et al. 1981). Alderman et al. (1981) eluted autologous IgG by thermal treatment of the old cells. They postulated the presence of age-related antigenic determinant on the membranes (senescent determinants) of aged RBCs and erythrocytes treated with Vibrio cholera neuraminidase (VCN).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Alderman EM, Fudenberg HH, Lovins RE (1980) Binding of immunoglobulin classes to sub-populations of human red blood cells separated by density gradient centrifugation. Blood 55:897–902

    Google Scholar 

  • Alderman EM, Fudenberg HH, Lovins RE (1981) Isolation and characterization of an age-related antigen present on senescent human red blood cells. Blood 58:341–349

    PubMed  CAS  Google Scholar 

  • Ashwell G, Morell AG (1974) The role of surface carbohydrates in the hepatic recognition and transport of circulating glycoproteins. Adv Ezymol 41:99–128

    CAS  Google Scholar 

  • Balduini CL, Sinigalia F, Ascari E, Balduini C (1981) Aging of rabbit red cells in vitro: membrane modifications and their possible role in red cell survival in vivo. Acta Haematol 65:263–269

    Article  PubMed  CAS  Google Scholar 

  • Berlin NI, Waldman TA, Weissman SM (1959) Life span of red blood cell. Physiol Rev 39:577–616

    PubMed  CAS  Google Scholar 

  • Durocher JH, Payne RC, Conrad MB (1975) Role of sialic acid in erythrocytes survival. Blood 45:11–20

    PubMed  CAS  Google Scholar 

  • Fischer DG, Koren HS (1981) Quantitative immune phagocytosis by macrophages. In: Herscowitz HB, Holden HT, Bellanti JA, Ghaffar A (eds) Manual of macrophage methodology. Dekker, New York, pp 259–264

    Google Scholar 

  • Friedenreich V (1928) Investigation into the Thomsen hemagglutination phenomenon. Acta Path Microbiol Scand 5:5–11

    Google Scholar 

  • Gattengo L, Fabid F, Bladier D, Cornillot P (1979) Physiological aging of red blood cells and changes in membrane carbohydrates. Biomedicine 30:194–199

    Google Scholar 

  • Gemsa D, Wooch, Fudenberg HH, Schemid R (1974) Stimulation of hemeoxygenase in macrophage and liver by endotoxin. J Clin Invest 53:647–651

    Article  PubMed  CAS  Google Scholar 

  • Halpern B, Bourden G (1971) Experimental immunohematological syndrome, having the characteristics of autoimmune hemolytic anemia induced in the mouse by administration of heated isologous red cells: critical importance of heating temperature. CR Acad Sci Ser D 273:2712–2717

    CAS  Google Scholar 

  • Huber H, Fudenberg HH (1968) Receptor sites of human monocytes for IgG. Int Arch Allergy Appl Immunol 34:18–24

    Article  PubMed  CAS  Google Scholar 

  • Hudson L, Hay FC (1976) Practical immunology. Blackwell Scientific Publications, London, pp 252–254

    Google Scholar 

  • Hunt JS, Bech ML, Wood GW (1981) Monocyte mediated erythrocyte destruction: a comparative study of current methods. Transfusion 21:735–738

    Article  PubMed  CAS  Google Scholar 

  • Kay MB (1975) Mechanism of removal of senescent cells by human macrophages in situ. Proc Natl Acad Sci USA 72:3521–3525

    Article  PubMed  CAS  Google Scholar 

  • Khansari N, Fudenberg HH (1984) Specificity of antigen induced helper factor for antibody synthesis in vitro. Immunol Commun 13:351–360

    PubMed  CAS  Google Scholar 

  • Kr von dem Borne AEG, Bechers D, Engelfriet CP (1977) Mechanism of red cell destruction mediated by non-complement binding IgG antibodies: the essential role in vivo of the Fc part of IgG. Br J Haematol 36:485

    Article  Google Scholar 

  • Murphy JR (1973) Influence of temperature and method of centrifugation on separation of erythrocytes. J Lab Clin Med 82:334–341

    PubMed  CAS  Google Scholar 

  • Nisonoff A, Rivers MM (1961) Recombination of a mixture of univalent antibody fragments of different specificity. Arch Biochem Biophys 93:460–462

    Article  PubMed  CAS  Google Scholar 

  • Nordt FJ (1980) Alterations in surface charge density versus changes in surface charge topography in aging red blood cells. Blut 40:233–238

    Article  PubMed  CAS  Google Scholar 

  • Perret G, Bladier D, Pre J, Cornillot PC (1978) Comparison of nucleated and enucleated asialylated erythrocytes survival time. Possible role of T-agglutinin. Comp Biochem Physiol 60:417–420

    Article  Google Scholar 

  • Platt D, Norwig P (1980) Biochemical studies of membrane glycoproteins during red cell aging. Mech Ageing Dev 14:119–126

    Article  PubMed  CAS  Google Scholar 

  • Springer GF, Desi PR (1982) Extent of desialylation of blood group MM, NN and MN antigens required for reactivity with human anti-T antibody and Arachis hypogaea. J Biol Chem 255:2744–2746

    Google Scholar 

  • Tenhunen R, Marver HS, Schmid R (1969) Microsomal hemeoxygenase: characterization of the enzyme. J Biol Chem 244:6388–6394

    PubMed  CAS  Google Scholar 

  • Van der Muelen FW, Van der Hart M, Fleer A, Kr von dem Borne AEG, Engelfriet CP, Van Loghem JJ (1978) The role of adherence to human mononuclear phagocytes in the destruction of red cells sensitized with non-complement binding IgG antibody. Br J Haematol 38:541

    Article  Google Scholar 

  • Victoria EJ, Mahan LC, Masouredis SP (1982) The IgG binding function of the normal red cell plasma membrane: identification of integral polypeptides that bind IgG. Br J Haematol 50:101–110

    Article  PubMed  CAS  Google Scholar 

Download references

Authors
  1. N. Khansari
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Instituts für Gerontologie, Universität Erlangen-Nürnberg, Flurstraße 17, D-8500, Nürnberg, Germany

    Dieter Platt (Direktor) (Direktor)

  2. 2. Medizinischen Klinik, Klinikum Nürnberg, Flurstraße 17, D-8500, Nürnberg, Germany

    Dieter Platt (Direktor) (Direktor)

Rights and permissions

Reprints and permissions

Copyright information

© 1988 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Khansari, N. (1988). Mechanism for Elimination of Senescent Red Blood Cells from Circulation. In: Platt, D. (eds) Blood Cells, Rheology, and Aging. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71790-1_8

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-71790-1_8

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71792-5

  • Online ISBN: 978-3-642-71790-1

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation