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Isolation and Characterization of Neuropeptides

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Neurochemical Techniques in Insect Research

Part of the book series: Springer Series in Experimental Entomology ((SSEXP))

Abstract

The recognition of neuropeptides as a major class of neurotransmitters has led to a set of revolutionary discoveries about chemical neurotransmission. Widely held ideas about the total number of neurotransmitters, the number of transmitters used by a single neuron, the anatomical structures over which transmitters act, and the array of postsynaptic events they produce have changed in the last 5 to 10 years, and in large measure studies of neuropeptides are responsible for those changes. Thus, it seems clear that neuropeptides are a class of neurotransmitters well worth studying, not only because of their inherent significance to the physiology of the nervous system, but also because modern tools available for the study of neuropeptides make them excellent models for the study of neurotransmission in general.

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Abbreviations

AKH:

adipokinetic hormone

c.c:

corpora cardiac

cDNA:

complementary DNA

DABS:

4-dimethylaminoazobenzene-4′-sulfonyl

ELH:

egg-laying hormone

FAB:

fast atom bombardment

GPC:

gel permeation chromatography

HPLC:

high performance liquid chromatography

MS:

mass spectrometry

pGlu:

pyroglutamate

PTHaa:

phenylthiohydantoin-amino acid

Å:

angstrom

mM :

millimolar

µ:

micron

nm:

nanometer

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Author information

Authors and Affiliations

  1. University of Chicago, Chicago, Illinois, USA

    M. H. Schaffer

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  1. M. H. Schaffer
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Editors and Affiliations

  1. Fachbereich Biologie/Chemie, Universität Osnabrück, Barbarastraße 11, 4500, Osnabrück, Germany

    Heinz Breer

  2. Department of Entomology, Division of Toxicology & Physiology, University of California at Riverside, 92521, Riverside, California, USA

    Thomas A. Miller

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© 1985 Springer-Verlag Berlin Heidelberg

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Schaffer, M.H. (1985). Isolation and Characterization of Neuropeptides. In: Breer, H., Miller, T.A. (eds) Neurochemical Techniques in Insect Research. Springer Series in Experimental Entomology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70045-3_3

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  • DOI: https://doi.org/10.1007/978-3-642-70045-3_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-70047-7

  • Online ISBN: 978-3-642-70045-3

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