Abstract
Since the 1970s tremendous progress has been made in the development of techniques which allow the study of the HLA system to be undertaken directly at the level of the gene. This new recombinant DNA technology makes possible the production of virtually unlimited quantities of any desired DNA segment for analysis. These techniques provide a powerful approach to the study of gene expression, and have greatly advanced our knowledge of the structure and function of the HLA system. Among the most useful techniques are:
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1.
The cutting of DNA into multiple fragments by restriction enzymes
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The transfer of size-separated fragments onto nitrocellulose filters
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The hybridization of fragments with specific, complementary radiolabeled probes
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4.
The isolation and cloning of specific fragments of interest
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5.
The localization of a given fragment to a particular position on the chromosome
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6.
The determination of the DNA sequence of a fragment
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References
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517
Sanger F (1981) Determination of nucleotide sequences in DNA. Science 214: 1205–1210
Gilbert W (1981) DNA sequencing and gene structure. Science 214: 1305–1312
Lemonnier FA, Malissen M, Goldstein P, et al (1982) Expression of human class I histocompatibility antigens on the surface of DNA-transformed mouse cells. Immunogenetics 16: 355–361
Rabourdin-Combe C, Mach B (1983) Expression of HLA- DR antigens at the surface of mouse L cells co-transfected with cloned human genes. Nature 303: 670–674
Steinmetz A, Hood L (1983) Genes of the major histocompatibility complex in mouse and man. Science 222: 727–733
Auffray C, Kuo J, DeMars R, Strominger JL (1983) A minimum of four human Class II α-chain genes are encoded in the HLA region of chromosome 6. Nature 304: 174–177
Cohen D, Paul P, Font M-P (1983) Analysis of HLA Class I genes with restriction endonuclease fragments: implications for polymorphism of the human major histocompatibility complex. Proc Natl Acad Sci USA 80: 6289 - 6292
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© 1984 Springer-Verlag Berlin Heidelberg
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Cohen, D., Robbins, E., Dausset, J. (1984). Recombinant DNA Technology: Its Use in the Study of HLA. In: Albert, E.D., et al. Histocompatibility Testing 1984. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69770-8_211
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DOI: https://doi.org/10.1007/978-3-642-69770-8_211
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-69772-2
Online ISBN: 978-3-642-69770-8
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