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Analysis of Cytolytic Clones Directed at Class I and Class II Antigens Using HLA Loss Mutants of a Lymphoblastoid Cell Line

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Histocompatibility Testing 1984

Abstract

One useful approach to analysis of the human major histocompatibility complex is to generate somatic cell variants with altered expression of HLA gene products. It is possible to generate not only a single HLA antigen loss, but also variants with simultaneous loss of two or three HLA antigens, by gamma ray irradiation. These HLA loss variants have enabled us to study the antigenic specificities detected by alloreactive responses in man.

This is paper # 389 from the Immunobiology Research Center, and was supported in part by NIH grants AI 17687, AI 18326, AM 13083, AI 19007, and the National Multiple Sclerosis Society grant # RG 1505-A-1 and the March of Dimes Birth Defects Foundation grant # 1–887

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References

  1. Kavathas P, Bach FH, DeMars R (1980) Gamma ray-induced loss of expression of HLA and glyoxalase I alleles in lymphoblastoid cells. Proc Natl Acad Sci USA 77: 4251–4255

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  2. Watson AJ, DeMars R, Trowbridge IS, Bach FH (1983) Detection of a novel human class II HLA antigen. Nature 304: 358–360

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Author information

Authors and Affiliations

  1. Immunobiology Research Center, University of Minnesota, Box 724 Mayo, Minneapolis, MN, 55455, USA

    N. Ohta, N. L. Reinsmoen, A. Anichini & F. H. Bach

Authors
  1. N. Ohta
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  2. N. L. Reinsmoen
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  3. A. Anichini
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  4. F. H. Bach
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Editor information

Editors and Affiliations

  1. Labor für Immungenetik, Kinderpoliklinik der Universität, Pettenkoferstraße 8a, 8000, München 2, Bundesrepublik Deutschland

    Ekkehard D. Albert

  2. Biometrische Abteilung, Diabetes Forschungsinstitut an der Universität, Auf’m Hennekamp 65, 4000, Düsseldorf 1, Bundesrepublik Deutschland

    Max P. Baur

  3. Institut für Blutgruppenserologie der Universität, Spitalgasse 4, 1090, Wien, Österreich

    Wolfgang R. Mayr

  4. Essen, Germany

    J. Bertrams  & H. Grosse-Wilde  & 

  5. Ulm, Germany

    S. Goldmann

  6. Heidelberg, Germany

    G. Opelz

  7. Bonn, Germany

    C. Rittner

  8. Munich, Germany

    D. J. Schendel , S. Scholz  & R. Wank ,  & 

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© 1984 Springer-Verlag Berlin Heidelberg

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Ohta, N., Reinsmoen, N.L., Anichini, A., Bach, F.H. (1984). Analysis of Cytolytic Clones Directed at Class I and Class II Antigens Using HLA Loss Mutants of a Lymphoblastoid Cell Line. In: Albert, E.D., et al. Histocompatibility Testing 1984. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69770-8_169

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  • DOI: https://doi.org/10.1007/978-3-642-69770-8_169

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-69772-2

  • Online ISBN: 978-3-642-69770-8

  • eBook Packages: Springer Book Archive

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