Segregation Analysis of PLT Reagents in Families

Abstract

From the data included in the central analysis of DP, 36 families could be used for segregation analysis. The numbers of families informative for each DP allele are listed in Table 1. Only two families were informative for DPwl, since primed lymphocyte (PL) reagent 20 did not function well in several of the larger experiments. For the DPw2 specificity, nine informative families were analyzed. In one family, one child carried the DPw2 specificity who did not have the expected HLA haplotype carrying this specificity in the parent and three other children. Since data on GLO was not available, a recombination event could not be confirmed. For the specificities DPw2-DPw4, one child in each of several families was found to lack an antigen, although inheriting the given HLA haplotype with which the DP specificity was associated in at least two other children.

Expected positives are based on the presence of the DR specificity to which the PLs were primed (i.e., DRI for PLs 1 – 3; DR2 for PLs 4 – 6; DR3 for PLs 7– 9)