Abstract
Depletion of hepatic glutathione in phenobarbital-induced rats following administration of various glutathione-depleting agents led to a strong enhancement of the spontaneous lipid peroxidation. This was evidenced both by the production of malondialdehyde and conjugated dienes. The peroxidative action was inhibited by the addition of either glutathione, dithiocarb or one of the following flavonoids: (+)-catechin, (−)-epicatechin, 3-0-methylcatechin, quercetin, taxifolin, rutin, naringin, and naringenin in a concentration-dependent manner. The I50-values lay between 1 μM for glutathione and 19 μM for naringenin. The least active flavonoids were those which lack the 3′-OH-group, i.e., naringin and naringenin. Thus, the 3′,4′-dihydroxy-grouping seems to be of major importance for the antioxidative action of the flavonoids investigated.
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Abbreviations
- Dithiocarb:
-
diethyldithiocarbamate
- GSH:
-
Glutathione
- LPO:
-
lipid peroxidation
- MDA:
-
malondialdehyde
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© 1982 Springer-Verlag
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Younes, M., Siegers, CP. (1982). Inhibition by Some Flavonoids of Enhanced Spontaneous Lipid Peroxidation in Vitro Due to Glutathione Depletion in Vivo. In: Chambers, C.M., Chambers, P.L. (eds) New Toxicology for Old. Archives of Toxicology, vol 5. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68511-8_62
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DOI: https://doi.org/10.1007/978-3-642-68511-8_62
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