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Nucleic Acids and Proteins in Plants II pp 46–74Cite as

Chromosomal DNA Sequences and Their Organization

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Part of the book series: Encyclopedia of Plant Physiology ((922,volume 14 / B))

Abstract

One of the most bewildering features about the nuclear DNA of higher plants is the variability in amount and type between different species. The haploid DNA contents of angiosperms ränge from about 0.5 to over 200 pg (Bennett and Smith 1976). Sometimes the Variation is extensive within a genus. For example, there is a threefold Variation in haploid DNA content within the genus Lathyrus without any Variation in chromosome number (Rees and Hazarike 1969) and a tenfold Variation in the genus Crepis (Jones and Brown 1976). The amount of DNA in Angiosperms at the lowest end of the ränge, i. e., about 0.5 pg is approximately five times the amount in Drosophila melanogaster, a fact which immediately provokes the conclusion that higher plants contain a considerable excess of DNA over what is needed to specify their biological complexity (Hinegardner 1976). The large amounts of DNA in plants and the extensive Variation in DNA content between species pose many problems of a technical as well as an intellectual nature for research on chromosomal DNA sequences.

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Abbreviations

C0t :

unit of DNA reassociation after melting (definition see p. 47)

HAP:

hydroxyapatite. Restriction enzymes

Bam HI:

Bacillus amyloliquefaciens

Bgl II:

Bacillusglobigii

Eco RI:

Escherichia coli RY 13

Hae III:

Haemophilus aegypticus

Hind III :

Haemophilusinfluenzae Rd

Hpa II :

Haemophilus parainfluenzae

Pst I :

Providencia stuartii

Sal I :

Streptomyces albus

Sma I :

Serratia marcescens

Xba I :

Xanthomonas badrii

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  1. R. B. Flavell
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Editors and Affiliations

  1. Institut für Biochemie der Pflanzen Halle, Akademie der Wissenschaften der DDR, Weinberg 3, Postfach 250, 401, Halle (Saale), Germany

    Benno Parthier

  2. Department of Botany, Science Laboratories, University of Durham, South Road, Durham, DH1 3LE, UK

    Donald Boulter

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Flavell, R.B. (1982). Chromosomal DNA Sequences and Their Organization. In: Parthier, B., Boulter, D. (eds) Nucleic Acids and Proteins in Plants II. Encyclopedia of Plant Physiology, vol 14 / B. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68347-3_2

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