Skip to main content

Isolation of Cell Membranes From Ascites Tumors

  • Chapter
Basic Exercises in Immunochemistry
  • 925 Accesses

Abstract

Various methods are described in the literature for the isolation of membranes of nucleated cells. The procedure described in this exercise is based on the method of Boone et al. (1973) with minor modifications as applied to the isolation of TA3-Ha murine ascites tumor membrane in the author’s laboratory (Nowotny et al., 1976).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Boone, C.W., Ford, E., Bund, E., Stuart, C., Lorenz, D.: J. Cell Biol. 41, 378, 1969

    Article  PubMed  CAS  Google Scholar 

  • Boone, C.W., Gerber, P., Brandschaft, P.B.: J. Nat. Cancer Inst, 50, 841, 1973

    PubMed  CAS  Google Scholar 

  • Heppel, L.A., Hilmoe, R.J.: In: Methods in enzymology. Colowick, S.P., Kaplan, N.O. (eds.): Vol. II, p. 546. New York: Academic Press

    Google Scholar 

  • Manson, L., Palm, J.: In: Advances in transplantation, J. Dausset et al. (eds.), Munksgaard, 1968

    Google Scholar 

  • Morton, R.K.: In: Methods in Enzymology. Colowick, S.P, Kaplan, N.O, (eds.), Vol. II, 1955 p 533. New York: Academic Press

    Chapter  Google Scholar 

  • Neville, D.M.: J. Biophys. Biochem. Cytol. 8, 413 (1960)

    Article  PubMed  Google Scholar 

  • Novotny, A., Butler, R.C., Grohsman, J., Keebler, C.; Ann N.Y. Acad. Sci. 276, 106, 1976

    Article  Google Scholar 

  • Wallach, D.F.H., Kamat, V.B.: Proc. Natl. Acad. Sci. USA 52, 721 (1964)

    Article  PubMed  CAS  Google Scholar 

  • Wallach, D.F.H., Ullrey, D.: Biochem. Biophys, Acta 88, 620 (1964)

    CAS  Google Scholar 

  • Wallach, D.F.H., Winzler, R.J.: Evolving strategies and tactics in membrane research, Berlin-Heidelberg-New York: Springer 1964

    Google Scholar 

  • Warren, L., Glick, M.C., Nass, M.K.: J. Cell Physiol, 68, 269, 1966

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

Copyright information

© 1979 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Nowotny, A. (1979). Isolation of Cell Membranes From Ascites Tumors. In: Basic Exercises in Immunochemistry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67356-6_17

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-67356-6_17

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-09453-1

  • Online ISBN: 978-3-642-67356-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics