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Handling Japanese Sea Urchins and Their Embryos

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The Sea Urchin Embryo

Abstract

A common physiologically balanced salt solution for marine animals, natural seawater must be used within several days after being drawn from the sea because its organic substances begin to be decomposed by bacteria. Kept in containers for more than one month, its organic substances are completely decomposed. Natural seawater can be used for culture after being filtered through active carbon particles and adjusted to pH 8.2 with sodium bicarbonate.

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References

  • BAKER, J.R., 1956. Cytological Technique. 2nd Ed. London: Methuen o., Ltd.

    Google Scholar 

  • FUJI, A., 1967. Ecological studies on the growth and food consumption of Japanese common littoral sea urchin, Strongylocentrotus intermedius (A. AGASSIZ). Mem. Fac. Fish. Hokkaido Univ., 15, 83–160.

    Google Scholar 

  • FUKûSHI, T., 1960a. The external features of the development of the sea urchin, Glyptocidaris crenularis (A. Agassiz). Bull. Mar. Biol. St. Asamushi, Tohoku Univ., 10, 57–63.

    Google Scholar 

  • FUKUSHI, T., 1960b. The formation of the echinus rudiment and the development of the larval form in the sea urchin, Temnopleurus hardwiki. Bull. Mar. Biol. St. Asamushi, Tohoku Univ., 10, 65–72.

    Google Scholar 

  • HARVEY, E.B., 1954. Electrical method of determining the sex of sea urchins. Nature, 173, 86.

    Article  Google Scholar 

  • HARVEY, E.B., 1956. The American Arbacia and Other Sea Urchins. Princeton: Princeton Univ. Press.

    Google Scholar 

  • HIRAI, E., 1963. On the breeding seasons of invertebrates in the neighborhood of the Marine Biological Station of Asamushi. Sci. Rep. Tôhoku Univ., Ser. IV (Biol.), 29, 369–375.

    Google Scholar 

  • IIDA, T., 1942. Uni no ran o ransô o kiridasazuni toru hôhô (A method obtaining sea urchin eggs without dissecting ovaries). Zool. Mag., 54, 2 80.

    Google Scholar 

  • IWATA, K.S., 1950. A method of determining the sex of sea urchins and of obtaining by electric stimulation. Annot. Zool. Jap., 23, 39–42.

    Google Scholar 

  • IWATA, K.S., 1962. A simplified electric method of determining the sex of sea urchins. Zool. Mag., 71, 301–302.

    Google Scholar 

  • IWATA, K.S., FUKASE, H., 1964a. Artificial spawning in sea urchins by acetylcholine. Biol. J. Okayama Univ., 10, 51–56.

    CAS  Google Scholar 

  • IWATA, K.S., FUKASE, H., 1964b. Comparison of discharge of the gametes by three artificial means in Sea urchins. Biol. J. Okayama Univ., 10, 57–64.

    Google Scholar 

  • KAWAMURA, K., 1970. On the development of the planktonic larvae of Japanese sea urchins, Strongylocentrotus intermedius and S. nudus. Sci. Rep. Hokkaido Fish. Exp. St., 12, 25–32.

    Google Scholar 

  • KOBAYASHI, N., 1969. Spawning periodicity of sea urchins at Seto. III. Tripneustes gratilla, Echinometra mathaei, Anthocidaris crassispina and Echinostrephus aciculatus. Sci. Eng. Rev. Doshisha Univ., 9, 254–269.

    Google Scholar 

  • KOBAYASHI, N., NAKAMURA, K., 1967. Spawning periodicity of sea urchins at Seto. II. Diadema setosum. Pub. Seto Mar. Biol. Lab., 15, 173–184.

    Google Scholar 

  • MATSUI, I., 1966. Uni no Zôshoku (The Propagation of the Sea Urchins). Tokyo: Nippon Shuisanshigen Hogo Kyokai.

    Google Scholar 

  • MOTOMURA, I., 1938. Effect of some salt solutions on the parthenogenetic membrane formation of sea urchin eggs. Sci. Rep. Tohoku Univ. Ser. IV (Biol.), 13, 85–88.

    Google Scholar 

  • MOTOMURA, I., 1941a. Materials of the fertilization membrane in the eggs of echinoderms. Sei. Rep. Tohoku Univ. Ser. IV (Biol.), 16, 345–363.

    Google Scholar 

  • MOTOMURA, I., 1941b. The sexual character of the sea urchin, Strongylocentrotus pulcherrimus (A. AGASSIZ). Sei. Rep. Tohoku Univ. Ser. IV (Biol.), 16, 431.

    Google Scholar 

  • MOTOMURA, I., 1953. Secretion of the sperm aggulutinin in the fertilized and denuded eggs of the sea urchin, Strongylocentrotus nudus. Sci. Rep. Tohoku Univ. Ser. IV (Biol.), 20, 93–97.

    CAS  Google Scholar 

  • MOTOMURA, I., 1954. Inhibition and acceleration of the toughening of the fertilization membrane in the sea urchin’s eggs. Sci. Rep. Tôhoku Univ. Ser. IV (Biol.), 20, 158–162.

    Google Scholar 

  • MOTOMURA, I., 1957. On the nature and localization of the third factor for the toughening of the fertilization membrane of the sea urchin egg. Sci. Rep. Tohoku Univ. Ser. IV (Biol.), 23, 168–181.

    Google Scholar 

  • MOTOMURA, I., 1958. Secretion of mucosubstance in the cleaving eggs of the sea urchin. Bull. Mar. Biol. St. Asamushi, 9, 79.

    Google Scholar 

  • MOTOMURA, I., 1960a. Secretion of mucosubstance in the gastrula of the sea urchin embryo. Bull. Mar. Biol. St. Asamushi, 10, 165–169.

    Google Scholar 

  • MOTOMURA, I., 1960b. On the structure of the cortical granule in the sea urchin egg. Sci. Rep. Tohoku Univ. Ser. IV (Biol.), 26, 367–374.

    Google Scholar 

  • MOTOMURA, I., 1963. Fixatives for electron microscopy. Bull. Mar. Biol. St. Asamushi, 11, 249–252.

    Google Scholar 

  • OKADA, K., 1950. An improved method of artificial insemination on some marine eggs. Sci. Rep. Tohoku Univ. Ser. IV (Biol.), 18, 262–269.

    CAS  Google Scholar 

  • OKADA, K., MIYAUCHI, H., 1954. Normal table of the early developmental stages on the sea urchin, Hemicentrotus pulcherrimus. J. Gakugei, Tokushima Univ., 5, 57–68.

    Google Scholar 

  • OKADA, K., MIYAUCHI, H., 1958. Sanshouni no shokihassei kijunzuhyô (Normal table of the early development of the sea urchin, Temnopleurus toreumaticus). J. Gakugei, Tokushima Univ., 8, 59–71.

    Google Scholar 

  • ONODA, K., 1932. Koshitaka-uni no yôsei to sono hentai (Larvae and their metamorphosis of Mespilia globulus). Zool. Mag., 44, 20–21.

    Google Scholar 

  • OSANAI, K., 1964a. Ecto- and endoplasmic relation in the partially activated egg of the sea urchin. Sci. Rep. Tohoku Univ. Ser. IV (Biol.), 30, 105–117.

    Google Scholar 

  • OSANAI, K., 1964b. Cortical granule as a body containing cytofertilizin in the sea urchin egg. Sci. Rep. Tohoku Univ. Ser. IV (Biol.), 30, 119–131.

    Google Scholar 

  • OSANAI, K., 1969. Behavior of pigment granules during early development in the eggs of sea urchin, Temnopleurus hardwiki. Ann. Rep. Fac. Ed. Univ. Iwate, 29, Pt. 3, 35–39.

    Google Scholar 

  • OSANAI, K., TAMURA, N., 1970. Uni-seishi chûshutsu-butsu no seirisayô (Physiological natures of the sperm extract of the sea urchin). Jap. J. Dev. Biol., 24, 80–81.

    Google Scholar 

  • RUNNSTRöM, J., KRISZAT, G., 1950. Action of periodate on the surface reactions attending the activation of the egg of the sea urchin, Psammechinus miliaris. Exp. Cell Res., 1, 355–370.

    Article  Google Scholar 

  • TAHARA, Y., OKADA, M., KOBAYASHI, N., 1958. Secondary sexual characters in Japanese sea urchins. Pub. Seto Mar. Biol. Lab., 7, 165–172.

    Google Scholar 

  • TAHARA, Y., OKADA, M., KOBAYASHI, N., 1960. Further notes on the sexual dimorphisms in Japanese sea urchins. Pub. Seto Mar. Biol. Lab., 8., 183–189.

    Google Scholar 

  • UTINOMI, H., 1954. A check list of echinoids found in the Kii regions. Pub. Seto Mar. Biol. Lab., 3 339–358.

    Google Scholar 

  • UTINOMI, H., 1960. Echinoides from Hokkaido and the neighbouring subarctic waters. Pub. Seto Mar. Biol. Lab., 8, 337–350.

    Google Scholar 

  • UTINOMI, H., 1965. Uni-kô (Echinoidea). New Illustrated Encyclopedia of the Fauna of Japan 3. Tokyo: Hokuryu-kan Pub. Co.

    Google Scholar 

  • YAMAMOTO, M., 1965. An experimental study on the cleavage of sea urchin eggs by means of the formation of the egg protuberance. Sci. Rep. Tôhoku Univ. Ser. IV (Biol.), 31, 55–62.

    Google Scholar 

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Authors
  1. K. Osanai
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Editors and Affiliations

  1. Universität Salzburg, Porschestr. 8/V, A-5020, Salzburg, Austria

    G. Czihak (Lehrkanzel für Genetik und Entwicklungsbiologie) (Lehrkanzel für Genetik und Entwicklungsbiologie)

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© 1975 Springer-Verlag Berlin · Heidelberg

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Osanai, K. (1975). Handling Japanese Sea Urchins and Their Embryos. In: Czihak, G. (eds) The Sea Urchin Embryo. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65964-5_3

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  • DOI: https://doi.org/10.1007/978-3-642-65964-5_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-65966-9

  • Online ISBN: 978-3-642-65964-5

  • eBook Packages: Springer Book Archive

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