Summary
An artificial symbiosis was established between diazotrophic Azomonas insignis and strawberry (Fragaria x ananassa). The partnership was created by in vitro techniques through callus induction and organogenesis. The basis of this partnership is the bacterial dependence on the plant’s metabolic activity, using maltose in the medium as a carbon and energy source which can be utilized by the plant cells only.
The presence of bacteria in the intercellular spaces of the callus tissues and re-generated plants was proven by microscopic techniques. Nitrogenase activity could also be detected in the plant tissues.
Preliminary experiments were carried out using a biolistic gun in order to ensure a higher incidence of bacterium introduction. This method may allow the incorporation of bacteria into the cells too.
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References
Berg RH, Vasil V, Vasil IK (1979) The biology of Azospirillum-sugarcane association. II. Ultrastructure. Protoplasma 101: 143–163
Carlson PS, Chaleff RS (1974) Forced association between higher plant and bacterial cells in vitro. Nature 252: 393–395
Karube I, Matsunaga T, Otomine Y, Suzuki S (1981) Nitrogen fixation by mobilized Azotobacter chroococcum. Enzyme Microb Technol 3: 309–312
Korányi P, Varga SzS, Preininger É, Gyurján I (1993) Symbiotic integration of diazo-trophic bacteria with plant cells in artificial cytobioses. Endocytobiology V 465–472
Kuhla J, Dingler Ch, Oelze J (1985) Production of extracellular nitrogen-containing components by Azotobacter vinelandiifixing dinitrogen in oxygen-controlled continuous culture. Arch Microbiol 141: 297–302
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assay with tobacco tissue cultures. Physiol Plant 15: 473–497
Newton JW, Wilson PW, Burns RH (1953) Direct demonstration of ammonia as an intermediate in nitrogen fixation by Azotobacter. J Biol Chem 204: 445–453
Varga SzS, Korányi P, Preininger É, Gyurján I (1994) Artificial associations between Daucusand nitrogen-fixing Azotobactercells in vitro. Physiol Plant 90: 786–790
Vasil V, Vasil IK, Zuberer DA, Hubbell DH (1979) The biology of Azospirillum-sugarcaneassociation. I. Establishment of association in vitro. Z Pflanzenphysiol 95: 141–147
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© 1997 Springer-Verlag Berlin Heidelberg
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Preininger, É., Koränyi, P., Gyurjän, I. (1997). Creation of Artificial Symbiosis Between Azotobacter and Higher Plants. In: Schenk, H.E.A., Herrmann, R.G., Jeon, K.W., Müller, N.E., Schwemmler, W. (eds) Eukaryotism and Symbiosis. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60885-8_39
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DOI: https://doi.org/10.1007/978-3-642-60885-8_39
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