Skip to main content

Pteridium aquilinum (L.) Kuhn (Bracken Fern): In Vitro Culture and the Production of Ecdysteroids

  • Chapter
Medicinal and Aromatic Plants VI

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 26))

Abstract

The bracken fern Pteridium aquilinum (L.) Kuhn of the family Pteridaceae belongs to the genus Pteridium Scopoli. This genus consists of five closely related species; according to some authors, the genus is monotypical, with only one polymorphous species (Dvořáková 1988). P. aquilinum is a deciduous fern up to 2 m high with stout, cylindrical petioles up to 1 m long, which grow from thick, black, creeping rhizomes. The petioles contain 10 to 20 veins, which form on the cut surface, the characteristic figure being reminiscent of the two-headed heraldic eagle. The rootstock is up to 12 mm thick, each branch usually producing only one leaf per year. Blades are triangular bi-to tripinnate, light green, lanceolate to oblong pinules, lobed at the base, with marginal sori (Fig. 1, Dostál 1989). The sporangia ripen from July to September, and the plant grows gregariously in open woods, on heath and moorland (Dvořáková 1988). This fern and its subspecies are distributed all over the world and it is an important cosmopolitan species (Toman and Felix 1978), appearing mostly in light acidophilic (often pine) forests on silicate substrates. Bracken has been known since 1753 (Dvořáková 1988) under the synonymum Pteris aquilina (L.), or Allosorus aquilinus (L.) C. since 1836 other synonyms include Pteris brevipes (Tausch 1836), or Eupteris aquilina (L.) (Newman 1845).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 189.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 249.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Agnew-Michael P, Lauren-Denis R (1991) Determination of ptaquiloside in bracken fern (Pteridium esculentum). J Chromatogr 538, 2:462–468

    Article  Google Scholar 

  • Baker-Edward A, Gaskin-Robyn E (1987) Composition of leaf epicuticular waxes of Pteridium subspecies. Phytochemistry 26, 10:2847–2848

    Article  Google Scholar 

  • Bandara BMR, Jayasinghe L, Karunaratne V, Wannigama GP, Bokel M, Kraus W, Sotheeswaran S (1989) Ecdysterone from stem of Dyploclisia glaucescens. Phytochemistry 28:1073–1075

    Article  CAS  Google Scholar 

  • Bell PR (1983) Nuclear bodies in the maturing egg of a fern, Pteridium aquilinum. J Cell Sci 60:109–116

    PubMed  CAS  Google Scholar 

  • Bergamasco R, Horn DHS (1983) Distribution and role of insect hormones in plants. In: Laufer H, Downer RGH (eds) Endocrinology of insects. Alan R Liss, New York, pp 627–654

    Google Scholar 

  • Breznovits A, Mohay J (1987) In vitro problems related to propagation of different fern species. Acta Hortic 212:427–431

    Google Scholar 

  • Breznovits A, Sheffield E (1990) Isolation and investigation of streptomycin resistant lines of the fern Pteridium aquilinum. In vitro Cell Dev Biol 26:301–304

    Article  CAS  Google Scholar 

  • Breznovits A, Vida G (1984) In vitro fern sporophytes. In: Novák FJ, Havel J. Doležel J (eds) Proc Int Symp Plant tissue and cell culture application to crop improvement. ČSAV, Prague, pp 211–214

    Google Scholar 

  • Bryan GT, Pamukcu MA (1982) Sources of carcinogens and mutagens in edible plants: production of urinary bladder and intestinal tumors by bracken fern (Pteridium aquilinum). In: Stich HF (ed) Carcinog Mutagens Environ, vol 1, CRC Press, Boca Raton, pp 75–82

    Google Scholar 

  • Callaghan TV, Sheffield E (1985) Pteridium aquilinum: weed or resource? Proc R Soc Edinb 86:461

    Google Scholar 

  • Camps F, Claveria E, Coll J, Macro M-P, Messeguer J, Melé E (1990) Ecdysteroid production in tissue cultures of Polypodium vulgare. Phytochemistry 29, 12:3819–3821.

    Article  Google Scholar 

  • Choung Ch-Ch, Yang Ch-B (1980) Studies on the utilization of bracken fern (Pteridium aquilinum) as a ruminant feed. I. Study on feeding bracken silage to sheep. Han’guk Chuksan Hak’hoe Chi 22, 4:275–281

    Google Scholar 

  • Dimitrova Ts, Benkova B (1987) Effectiveness of herbicides in controling bracken (Pteridium aquilinum Kuehn). Pochvozn Agrokhim Rastit Zasht 22, 2:108–112

    Google Scholar 

  • Dix PJ, Joo F, Maliga P (1977) A cell line of Nicotiana sylvestris with resistance to kanamycin and streptomycin. Mol Gen Genet 157:285–290

    Article  CAS  Google Scholar 

  • Dostál J (1989) Pteridium Gleditsch. New flora of the ČSSR, Academia, Praha, pp 62–63

    Google Scholar 

  • Dvořáková M (1988) Pteridium aquilinum (L.) Kuhn — hasivka orličí. In: Hejný S, Slavík B (eds) Flora of the Czech socialist republic, vol 1. Academia, Praha, pp 235–236

    Google Scholar 

  • Elmore HW, Webb D, Ripley B, White AR (1989) Effects of sodium selenite on the growth of bracken fern root callus. Ann Bot (Lond) 64, 3:289–291

    Google Scholar 

  • Evans WCh, Patel MC, Koohy Z (1982) Acute bracken poisoning in homogastric and ruminant animals. Proc Soc Edinb Sect B (Biol Sci) 81, 1-2:29–64

    CAS  Google Scholar 

  • Evans WCh, Korn T, Hatori S, Zoshihira K, Fukuoka M (1983) Chemical and toxicological studies on bracken fern, Pteridium aquilinum var. latiusculum. VIII. The inability of bracken extracts containing pterosins to cause cattle bracken poisoning. J Pharmacobio-Dyn 6, 12:936–940

    Google Scholar 

  • Fenwick GR (1989) Bracken (Pteridium aquilinum) — toxic effects and toxic constituents. J Sci Food Agric 46, 2:147–173

    Article  Google Scholar 

  • Forrest V, Cody T, Caruso J, Warshawsky D (1989) Influence of the carcinogenic pollutant benzo (a) pyrene on plant development: fern gametophytes. Chem-Biol Interact 72, 3:295–307

    Article  Google Scholar 

  • Fukuoka M (1982) Chemical and toxicological studies on bracken fern, Pteridium aquilinum var. latiusculum. VI. Isolation of 5-O-caffeoylshikimic acid as as antithiamine factor. Chem Pharm Bull 30, 9:3219–3224

    Google Scholar 

  • Galbraith MN, Horn DHS (1966) An insect-moulting hormone from a plant. Chem Commun 24:905–906

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158

    Article  PubMed  CAS  Google Scholar 

  • Gaskin-Robyn E, Kirkwood RC (1989) The effect of certain nonionic surfactants on the uptake and translocation of herbicides in bracken (Pteridium aquilinum) (L.) Kuhn. In: Chow PNP (ed) Adjuvants Agrochem. CRC Press, Boca Raton, pp 129–139

    Google Scholar 

  • Hallam ND, Herrman C, Richardson RG (1987) Effects of asulam and glyphospate on the leaves and rhizomes of Pteridium esculentum. Plant Prot Q 2, 2:59–66

    CAS  Google Scholar 

  • Harmatha J (1990) Plant phytoecdysones and their ecological function in plant-insect interaction. Bull Cs Bot Soc Prague 25, 8:59–63

    Google Scholar 

  • Harmatha J, Sláma K, Píš J (1990) Action of some derivatives of phytoecdysones on insects. In: Szentesi A, Jermy T. (eds) Proc 7th Int Conf Insect-plant interactions, Budapest, Symp Biol Hung 39. Akadémiai Kiadó, Budapest, pp 471–472

    Google Scholar 

  • Hikino H, Nomoto K, Takemoto T (1971) Occurrence of insect moulting substances ecdysterone and inokosterone in callus tissues of Achiranthes. Phytochemistry 10:438–439

    Article  Google Scholar 

  • Hinshalwood AM, Kirkwood RC (1988) The effect of simultaneous application of ethephon or 2,4-D on the absorption, translocation and biochemical action of asulam in bracken fern [Pteridium aquilimm (L.) Kuhn]. Can J Plant Sci 68, 4:1025–1034

    Article  Google Scholar 

  • Hirono I (1986) Carcinogenic principles isolated from bracken fern. CRC Crit Rev Toxicol 17, 1:1–22

    Article  Google Scholar 

  • Hirono I, Yamada K (1987) Bracken fern. Bioact Mol 2:87–120

    CAS  Google Scholar 

  • Horn DHS, Bergamasco R (1985) Chemistry of ecdysteroids. In:Kerkut GA, Gilbert LI (eds) Comprehensive insect physiology, biochemistry and pharmacology, vol 7. Pergamon Press Oxford, pp 185–235

    Google Scholar 

  • Jizba J, Herout V (1967) Isolation of constituents of common polypody rhizomes (Polypodium vulgare). Plant substances XXVI. Collect Czech Chem Commum 32:2867–2874

    CAS  Google Scholar 

  • Jizba J, Herout V, šorm F (1967a) Isolation of ecdysterone (crustecdysone) from Polypodium vulgare L. rhizomes. Tetrahedron Lett 18:1689–1691

    Google Scholar 

  • Jo J-S, Kim S-K, Lee K-H, Kwon T-W (1981) Physico-chemical properties of bracken (Pteridium aquilinum) root starch. Han’guk Sikp’um Kwahakhoe Chi 13, 2:133–141

    Google Scholar 

  • Kaplanis JN, Thompson MJ, Robbins WE, Bryce BM (1967) Insect hormones: alpha ecdysone and 20-hydroxyecdysone in bracken fern. Science 157:1436–1438

    Article  PubMed  CAS  Google Scholar 

  • Khatoon Kh (1987) The influence of hydroxyurea treatment on various stages of the life cycle of Pteridium aquilinum L. Pak J Bot 19, 1:43–52

    Google Scholar 

  • Kirkwood RC, Veerasekaran P, Fletcher WW (1982) Studies on the mode of action of asulam in bracken (Pteridium aquilinum L. Kuhn). Proc R Soc Edinb Sect B (Biol Sci) 81, 1-2:85–96

    Google Scholar 

  • Křížek M, Kubíčková M (1989) Toxicity of Pteridium aquilinum for farm animals. Veterinářství 39, 4:169–170

    Google Scholar 

  • Lafont R, Bouthier A, Wilson ID (1991) Phytoecdysteroids: Structures, occurrence, biosynthesis and possible ecological significance. In: Hrdý I (ed) Proc Conf Insect Chem Ecol, Tábor 1990. Academia Praha and SPB Acad Publ. The Hague, pp 197–214

    Google Scholar 

  • Lev SV, Zakirova RP, Saatov Z, Gorovits MB, Abubakirov NK (1989) Ecdysteroids from tissue and cell cultures of Ajuga turkestanica. Khim Prir Soedin 1:51–52

    Google Scholar 

  • Macek T, Vaněk T, Svatoš A, Breznovits A, Harmatha J (1989) Production of ecdysteroids by in vitro culture of cells of the fern Pteridium aquilinum. In: Vlahov R (ed) Proc Conf Chem Biotechnol Biol Act Nat Prod, Varna vol 4. Jusautor, Sofia, pp 238–242

    Google Scholar 

  • Maliga P, Breznovits A, Márton L (1973) Streptomycin-resistant plants from callus cultures of haploid tobacco. Nature New Biol 244:29–30

    PubMed  CAS  Google Scholar 

  • Maliga P, Breznovits A, Márton L, Joó F (1975) Non-Mendelian streptomycin-resistant tobacco mutants with altered chloroplasts and mitochondria. Nature 255:401–402

    Article  PubMed  CAS  Google Scholar 

  • Marc J, Gunning BES (1986) Immunofluorescent localization of csytoskeletal tubulin and actin during spermatogenesis in Pteridium aquilinum (L.) Kuhn. Protoplasma 134, 2-3:163–177

    Article  Google Scholar 

  • Matoba M, Saito E, Saito K, Koyama K, Natori S, Matusushima T, Takimoto M (1987) Assay of ptaquiloside, the carcinogenic principle of bracken, Pteridium aquilinum, by mutagenicity testing in Salmonella typhimurium. Mutagenesis 2, 6:419–423

    Article  Google Scholar 

  • Matsumoto T, Tanaka N (1991) Production of phytoecdysteroids by hairy root cultures of Ajuga reptans var. atropurpurea. Agric Biol Chem 55, 4:1019–1025

    Article  Google Scholar 

  • McMorris TC, Voeller B (1971) Ecdysones from gametophytic tissues of a fern. Phytochemistry 10:3253–3254

    Article  CAS  Google Scholar 

  • Moore GT (1903) Methods for growing pure cultures of algae. J Appl Microsc 6:2309–2313

    Google Scholar 

  • Mori H, Sugie S, Hirono I, Yamada K, Niwa H, Ojika M (1985) Genotoxicity of ptaquiloside, a bracken carcinogen, in the hepatocyte primary culture/DNA-repair test. Mutat Res 143, 1-2:75–78

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nakanishi K, Koreeda M, Sasaki S, Chang ML, Hsu HY (1966) Insect hormones. The structure of ponasterone A, an insect moulting hormone from the leaves of Podocarpus nakaii Hay. Chem Commum 24:915–917

    Google Scholar 

  • Newman (1845) Eupteris aquilina (L.). Phytologist 2:278

    Google Scholar 

  • Niwa H, Ojika M, Wakamatsu K, Yamada K, Hirono I, Matsushita K (1983) Ptaquiloside, a novel norsesquiterpene glucoside from bracken, Pteridium aquilinum var. latiusculum. Tetrahedron Lett 24,38:4117–4120

    Article  Google Scholar 

  • Ogunbiyi AL (1987) Aspects of the toxicology of the bracken fern (Pteridium aquilinum) Avail Univ Microfilms Int Order No BRDX80047, Univ Surrey, Surrey, 230 pp

    Google Scholar 

  • Ojika M, Wakamatsu K, Niwa H, Yamada K, Hirono I (1984) Isolation and structures of two new p-hydroxystyrene glycosides, ptelasotide-A and-B from bracken, Pteridium aquilinum var. latiusculum, and synthesis of ptelatoside A. Chem Lett 3:397–400

    Google Scholar 

  • Ojika M, Kigoshi H, Koyama H, Niwa H, Yamada K (1985) Studies on Pteridium aquilinum var. latiusculum. IV. Isolation of three p-hydroxystyrene glycosides and an efficient method for the isolation of ptaquiloside, an unstable bracken carcinogen. J Nat Prod 48, 4:634–637

    Google Scholar 

  • Ojika M, Wakamatsu K, Niwa H, Yamada K (1987) Ptaquiloside, a potent carcinogen isolated from bracken fern, Pteridium aquilinum var. latiusculum. Structure elucidation based on chemical and spectral evidence, and reactions with amino acids, nucleosides and nucleotides. Tetrahedron 43, 22:5261–5274

    Google Scholar 

  • Parry DW, Hodson MJ, Newman RH (1985) The distribution of silicon deposits in the fronds of Pteridium aquilinum L. Ann Bot (Lond) 55, 1:77–83

    Google Scholar 

  • Partanen CR (1972) Comparison of gametophytic callus and tumor tissues of Pteridium aquilinum. Bot Gaz 133:287–292

    Article  Google Scholar 

  • Partanen CR (1980a) Geographic variation in the frequency of spontaneous tumors on fern gametophytes. Nat Geogr Soc Res Rep 12:535–540

    Google Scholar 

  • Partanen CR (1980b) Filter paper as a support and carrier for plant protoplast cultures. In vitro 17:77–80

    Google Scholar 

  • Partanen CR, Power JB, Cocking EC (1980) Isolation and division of protoplasts of Pteridium aquilinum. Plant Sci Lett 17:333–338

    Article  Google Scholar 

  • Pinzon SR, Rubiano RA (1986) A preliminary study of Pteridium aquilinum. Chemical constituents and toxicological action. Rev Colomb Cienc Quim-farm 15:31–35

    Google Scholar 

  • Pis J, Harmatha J (1992) Phenylboronic acid as a versatile derivatization agent for chromatography of ecdysteroids. J Chromatogr 596, 2:271–275

    Article  Google Scholar 

  • Píš J, Vaisar T (1992) Fast atom bombardment mass spectrometry of ecdysteroids: derivatization reaction on the probe tip. In: Rees HH (ed) Proc Xth Ecdysone Worksh. Liverpool Univ Press, Liverpool, p 45

    Google Scholar 

  • Píš J, Harmatha J, Sláma K (1991) Action of some new synthetic derivatives of phytoecdysones on insects. In: Hrdý I (ed) Proc Conf Insect Chem Ecol, Tαbor 1990. Academia Praha and SPB Acad Publ, The Hague, pp 227–234

    Google Scholar 

  • Ravishankar GA, Mehta AR (1979) Control of ecdysterone biogenesis in tissue culture of Trianthema portulacastrum. J Nat Prod 42:152–158

    Article  CAS  Google Scholar 

  • Rees HH, Isaac RE (1985) Biosynthesis and metabolism of ecdysteroids and methods of isolation and identification of the free and conjugated compounds. In:Law JH, Rilling HC (eds) Methods in enzymology, vol 111. Academic Press, New York, pp 377–409

    Google Scholar 

  • Saito K, Nagao T, Matoba M, Koyama K, Natori S, Murakami T, Saiki Y (1989) Chemical assay of ptaquiloside, the carcinogen of Pteridium aquilinum, and the distribution of related compounds in the Pteridaceae. Phytochemistry 28, 6:1605–1611

    Article  Google Scholar 

  • Saito T, Mochizuki D (1986) Isolation of two active glucosides, braxin A1 and A2, from rhizomes of bracken fern. J Toxicol Sci 11, 1:15–27

    Article  Google Scholar 

  • Schreiner IH (1980) Cyanogenesis and the herbivorous insects of bracken fern (Pteridium aquilinum. Avail Univ Microfilms Int Order No 8102969 Cornell Univ, Ithaca, NY, 225 pp

    Google Scholar 

  • Sláma K (1979) Insect hormones and antihormones in plants. In: Rosenthal GA, Janzen DH (eds) Herbivores: their interaction with secondary plant metabolites. Academic Press, New York, pp 683–700

    Google Scholar 

  • Steeves TA, Sussex IM, Partanen CR (1955) In vitro studies on abnormal growth of the bracken fern. Am J Bot 42:232–245

    Article  Google Scholar 

  • Sussex IM, Steeves TA (1953) Experimental and analytical studies of Pteridophytes 21. Ann Bot 17:395–401

    CAS  Google Scholar 

  • Svatoš A, Macek T (1993) The rate of ecdysteriod production in suspension cultured cells of the fern Pteridium aquilinum. Phytochemistry (submitted)

    Google Scholar 

  • Tanaka N, Satake T, Takahashi A, Mochizuki M, Murakami T, Saiki Y, Yang JZ, Chen CM (1982) Chemical and chemotaxonomical studies of ferns. XXXIX. Chemical studies on the constituents of Pteris bella tagawa and Pteridium aquilinum subsp. wightianum (Wall) Shich. Chem Pharm Bull 30, 10:3640–3646

    Google Scholar 

  • Tan-Muy-Keng (1991a) Analysis of Pteridium ribosomal RNA sequences by rapid direct sequencing. Plant Mol Biol 17, 2:221–227

    Google Scholar 

  • Tan-Muy-Keng (1991b) Evolutionary studies of the chloroplast genome in Pteridium. Plant Sci 77, 1:81–92

    Google Scholar 

  • Tausch (1836) Pteris brevipes. Flora 19:427

    Google Scholar 

  • Taylor JA (1986) The bracken problem: a local hazard and global issue. In: Smith RT, Taylor JA (eds) Bracken: ecology, land use and control technology. Parthenon Press, Lancastershire, pp 21–41

    Google Scholar 

  • Toman J, Felix S (1978) Plants and animals. Octopus, London, pp 42–43

    Google Scholar 

  • Tomas J, Camps F, Claveria E, Coll J, Melé E, Messeguer J (1991) Phytoecdysteriod production in Ajuga reptans L. in vivo and in vitro cultures. In: Drašar P. Černý I (eds) Abstr 14th Conf on Isoprenoids, Třeboň UOCHB, ČSAV, Prague, pp 104–105

    Google Scholar 

  • Tomas J, Camps F, Claveria E, Coll J, Melé E, Messeguer J (1992) Composition and location of phytoecdysteroids in Ajuga reptans in vivo and in vitro cultures. Phytochemistry 31, 5:1585–1591

    Article  Google Scholar 

  • Tsapalova IE, Plotnikova BV (1981) Chemical composition of fresh and salted ferns. Konservn Ovoshchesush Prom St 6:42–43

    Google Scholar 

  • Vaisar T, Píš J (1993) Cyclic boronoates in mass spectrometry of ecdysteroids. Rapid Comm Mass Spectrom 7, 1:46–52

    Article  Google Scholar 

  • Vaisar T, Vaněk T (1990) Reversed-phase high-performance liquid Chromatographic separation of 5beta, 20-dihydroxy-ecdysone and 20-hydroxyecdysone on a beta-cyclodextrin-bonded stationary phase. J Chromatogr 547:443–446

    Google Scholar 

  • Van der Hoeven JCM, Lagerweij WJ, Posthumus MA, van Veldhuizen A, Holterman HAJ (1983) Aquilide A, a new mutagenic compound isolated from bracken fern [Pteridium aquilinum (L.) Kuhn]. Carcinogenesis (Lond) 4, 12:1587–1590

    Article  Google Scholar 

  • Vaněk T, Macek T, Vaisar T, Breznovits A (1990) Production of ecdysteroids by cell culture of pteridium aquilinum. Biotechnol Lett 12, 10:727–730

    Article  Google Scholar 

  • Vereskovskii VV, Chekalinskaya II, Paskina GV (1983) The dynamics of ecdysterone content in some Rhaponticum Ludw. species. Rastit Resur 19:60

    CAS  Google Scholar 

  • Watson MB, White AR, Elmore HW (1990) Extracellular polysaccharides from Pteridium aquilinum suspension cultures. Phytochemistry 29, 12:3815–3818

    Article  Google Scholar 

  • West TM, Richardson WG (1985) Response of bracken (Pteridium aquilinum) in pots to various herbicide treatments. Test Agrochem Cult 6:108–109

    CAS  Google Scholar 

  • Whittier DP, Pratt LH (1971) The effect of light quality on the induction of apogamy in prothalli of Pteridium aquilinum. Planta 99:174–178

    Article  Google Scholar 

  • Wilson ID, Morgan ED, Murphy SJ (1990) Solid phase extraction of ecdysteroids using immobilized phenylboronic acid. Anal Chim Acta 236:145

    Article  CAS  Google Scholar 

  • Wolf PG, Haufler ChH, Sheffield E (1987) Electrophoretic evidence for genetic diploidy in the bracken fern (Pteridium aquilinum). Science 236(4804):947–949

    Article  PubMed  CAS  Google Scholar 

  • Zhang WD (1985) Carcinogenicity of bracken fern (Pteridium aquilinum). Zhonghua Yixue Zazhi 65, 7:435–485

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1994 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Macek, T., Vaněk, T. (1994). Pteridium aquilinum (L.) Kuhn (Bracken Fern): In Vitro Culture and the Production of Ecdysteroids. In: Bajaj, Y.P.S. (eds) Medicinal and Aromatic Plants VI. Biotechnology in Agriculture and Forestry, vol 26. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57970-7_20

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-57970-7_20

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-63420-8

  • Online ISBN: 978-3-642-57970-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics