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Interferometrical Microscope for Investigations of Biological Objects

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Optics and Lasers in Biomedicine and Culture

Part of the book series: Series of the International Society on Optics Within Life Sciences ((3718,volume 5))

Abstract

In the present work the results for multidimensional visualisation of cells and chromosomes by computerised phase-stepping laser interferometry are presented. The set-up used is based on a Mach-Zender interferometer with projection microscope (magnification up to 600x) in one of the arms and a reference five-steps shifted beam in the other. High resolution CCD camera in measurement mode with 256 grey levels is used. The recordings are performed with different wavelengths in the red (He-Ne laser), green and blue (Ar+ laser) spectral regions. The distortions of the optical elements are omitted by subtracting images of the objects and/or reference plane. Essential features of the system are the optico-electronical feed-back and the specialised software for recording, digital processing and visualisation of the images.

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References

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Author information

Authors and Affiliations

  1. Central Laboratory of Optical Storage and Processing of Information to the Bulgarian Academy of Sciences, Sofia, 1113, P.O.Box 95, Bulgaria

    N. Metchkarov & V. Sainov

Authors
  1. D. Tontcheva
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  2. I. Sainova
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  3. N. Metchkarov
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  4. V. Sainov
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Editor information

Editors and Affiliations

  1. FORTH-IESL, PO Box 1527, GR 71110, Heraklion, Greece

    Costas Fotakis , Theodore G. Papazoglou  & Costas Kalpouzos ,  & 

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© 2000 Springer-Verlag Berlin Heidelberg

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Tontcheva, D., Sainova, I., Metchkarov, N., Sainov, V. (2000). Interferometrical Microscope for Investigations of Biological Objects. In: Fotakis, C., Papazoglou, T.G., Kalpouzos, C. (eds) Optics and Lasers in Biomedicine and Culture. Series of the International Society on Optics Within Life Sciences, vol 5. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56965-4_60

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  • DOI: https://doi.org/10.1007/978-3-642-56965-4_60

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-63073-6

  • Online ISBN: 978-3-642-56965-4

  • eBook Packages: Springer Book Archive

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