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Single Shot, Laser Plasma X-Ray Contact Microscopy of Chlamydomonas

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Optics and Lasers in Biomedicine and Culture

Part of the book series: Series of the International Society on Optics Within Life Sciences ((3718,volume 5))

Abstract

Images of living green alga chlamydomonas were obtained using shoft X-rays in the water window (2.3–4.4 nm). The specimen was placed on a photoresist, which acts as the recording medium. Successful biological imaging is based on the different absorption caused by water and the carbon-rich proteins of the living biological specimens. Atomic force and scanning electron microscopy of the biological image clearly showed the flagella and flagella intersections of the motile green alga, Chlamydomonas, recorded in the photoresist suggesting a lateral resolution better than 150nm.

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Authors and Affiliations

  1. Theoretical and Physical Chemistry Institute, National Hellenic Research Foundation, 48 Vas. Constantinou Av. Athens, 11635, Greece

    A. C. Cefalas, E. Sarantopoulou & Z. Kollia

  2. Institute of Microelectronics, NCSR “Demokritos”, 15310, Ag. Paraskevi, Greece

    P. Argitis

  3. School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, TW20 0EX, UK

    T. W. Ford, A. Marranca & A. D. Stead

  4. Rutherford Appleton Laboratory, Central Laser Facility, Chilton, Didcot, Oxon, OX11 0QX, UK

    C. N. Danson, J. Knott & D. Neely

Authors
  1. A. C. Cefalas
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  2. P. Argitis
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  3. E. Sarantopoulou
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  4. Z. Kollia
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  5. T. W. Ford
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  6. A. Marranca
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  7. A. D. Stead
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  8. C. N. Danson
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  9. J. Knott
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  10. D. Neely
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Editor information

Editors and Affiliations

  1. FORTH-IESL, PO Box 1527, GR 71110, Heraklion, Greece

    Costas Fotakis , Theodore G. Papazoglou  & Costas Kalpouzos ,  & 

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© 2000 Springer-Verlag Berlin Heidelberg

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Cefalas, A.C. et al. (2000). Single Shot, Laser Plasma X-Ray Contact Microscopy of Chlamydomonas . In: Fotakis, C., Papazoglou, T.G., Kalpouzos, C. (eds) Optics and Lasers in Biomedicine and Culture. Series of the International Society on Optics Within Life Sciences, vol 5. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56965-4_53

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  • DOI: https://doi.org/10.1007/978-3-642-56965-4_53

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-63073-6

  • Online ISBN: 978-3-642-56965-4

  • eBook Packages: Springer Book Archive

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