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Expression of Mutant Gpr1p in Yarrowia lipolytica and Its Effect on Growth on Different Media

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Non-Conventional Yeasts in Genetics, Biochemistry and Biotechnology

Part of the book series: Springer Lab Manuals ((SLM))

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Abstract

To express a mutated gene, causing sensitivity to acetic acid, and to study the effects on growth of transformants on acetic acid/acetate containing media.

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References

  • Augstein A (2001) Molekularbiologische Charakterisierung und funktionelle Analyse des GPR1-Genproduktes in der Hefe Yarrowia lipolytica. Dissertation, Dresden Technical University, Germany

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  • Le Dali MT, Nicaud JM, Gaillardin C (1994) Multiple-copy integration in the yeast Yarrowia lipolytica. Yeast 15:1645–16

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  • Tzschoppe K, Augstein A, Bauer R, Kohlwein SD, Barth G (1999) Trans-dominant mutations in the GPRl gene cause high sensitivity to acetic acid and ethanol in the yeast Yarrowia lipolytica. Yeast 15:1645–1656

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Author information

Authors and Affiliations

  1. Institute of Microbiology, Dresden Technical University, Mommsenstrasse 13, 01062, Dresden, Germany

    Marcus Gentsch, Margret Kuschel & Gerold Barth

Authors
  1. Marcus Gentsch
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  2. Margret Kuschel
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  3. Gerold Barth
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Editor information

Editors and Affiliations

  1. Institut für Biologie IV, RWTH Aachen, Worringer Weg, 52056, Aachen, Germany

    Klaus Wolf

  2. Martin-Luther Universität, Institut für Genetik, Weinsbergweg 10, 06095, Halle, Germany

    Karin Breunig

  3. Technische Universität Dresden, Institut für Mikrobiologie, Mommsenstr. 13, 01062, Dresden, Germany

    Gerold Barth

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© 2003 Springer-Verlag Berlin Heidelberg

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Gentsch, M., Kuschel, M., Barth, G. (2003). Expression of Mutant Gpr1p in Yarrowia lipolytica and Its Effect on Growth on Different Media. In: Wolf, K., Breunig, K., Barth, G. (eds) Non-Conventional Yeasts in Genetics, Biochemistry and Biotechnology. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-55758-3_61

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  • DOI: https://doi.org/10.1007/978-3-642-55758-3_61

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-44215-8

  • Online ISBN: 978-3-642-55758-3

  • eBook Packages: Springer Book Archive

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