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Experimental In Vivo Approaches of Pulp Regeneration

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The Dental Pulp

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Abstract

Stem cell therapy is a potential strategy to regenerate pulp/dentin complex, enabling conservation and restoration of teeth. An alternate approach is acellular morphogen therapy with homing/migration factors or morphogenetic signaling molecules. This section introduces experimental models of ectopic and orthotopic approaches for pulp regeneration including stem cell therapy and acellular morphogen therapy with or without stem/progenitor cells and homing/migration factors in extracellular matrix scaffold. Furthermore, in the orthotopic approach, there are two methods to inject stem/progenitor cells and homing/migration factors: one is tooth extraction method (injection ex vivo following tooth extraction) and another is non-tooth extraction method (injection in vivo without tooth extraction). These experimental approaches for pulp regeneration demonstrate the efficacy and safety of the regenerative therapies in preparation for the initiation of clinical trials.

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References

  1. Matchou P, Reit C. Non-surgical retreatment. In: Bergenholtz G, Horsted-Bindslev P, Reit C, editors. Textbook of endodontology. 1st ed. Oxford: Wiley-Blackwell; 2003. p. 300–10.

    Google Scholar 

  2. Wilcox LR, Van Surksum R. Endodontic retreatment in large and small straight canals. J Endod. 1991;17(3):119–21.

    Article  PubMed  Google Scholar 

  3. Fuss Z, Lustig J, Katz A, Tamse A. An evaluation of endodontically treated vertical root fractured teeth: impact of operative procedures. J Endod. 2001;27(1):46–8.

    Article  PubMed  Google Scholar 

  4. Nakashima M, Akamine A. The application of tissue engineering to regeneration of pulp and dentin in endodontics. J Endod. 2005;31(10):711–8.

    Article  PubMed  Google Scholar 

  5. Nakashima M, Iohara K, Sugiyama M. Human dental pulp stem cells with highly angiogenic and neurogenic potential for possible use in pulp regeneration. Cytokine Growth Factor Rev. 2009;20(5–6):435–40.

    Article  PubMed  Google Scholar 

  6. Huang GT. Apexification: the beginning of its end. Int Endod J. 2009;42(10):855–66.

    Article  PubMed  Google Scholar 

  7. Mooney DJ, Powell C, Piana J, Rutherford B. Engineering dental pulp-like tissue in vitro. Biotechnol Prog. 1996;12(6):865–8.

    Article  PubMed  Google Scholar 

  8. Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A. 2000;97(25):13625–30.

    Article  PubMed Central  PubMed  Google Scholar 

  9. Thibodeau B, Teixeira F, Yamauchi M, Caplan DJ, Trope M. Pulp revascularization of immature dog teeth with apical periodontitis. J Endod. 2007;33(6):680–9.

    Article  PubMed  Google Scholar 

  10. Thomson A, Kahler B. Regenerative endodontics–biologically-based treatment for immature permanent teeth: a case report and review of the literature. Aust Dent J. 2010;55(4):446–52.

    Article  PubMed  Google Scholar 

  11. Andreasen JO, Bakland LK. Pulp regeneration after non-infected and infected necrosis, what type of tissue do we want? A review. Dent Traumatol. 2011;28(1):13–8.

    Article  PubMed  Google Scholar 

  12. Nosrat A, Seifi A, Asgary S. Regenerative endodontic treatment (revascularization) for necrotic immature permanent molars: a review and report of two cases with a new biomaterial. J Endod. 2011;37(4):562–7.

    Article  PubMed  Google Scholar 

  13. Chen MY, Chen KL, Chen CA, Tayebaty F, Rosenberg PA, Lin LM. Responses of immature permanent teeth with infected necrotic pulp tissue and apical periodontitis/abscess to revascularization procedures. Int Endod J. 2012;45(3):294–305.

    Article  PubMed  Google Scholar 

  14. Law AS. Considerations for regeneration procedures. J Endod. 2013;39(3 Suppl):S44–56.

    Article  PubMed  Google Scholar 

  15. Trope M. Treatment of the immature tooth with a non-vital pulp and apical periodontitis. Dent Clin North Am. 2010;54(2):313–24.

    Article  PubMed  Google Scholar 

  16. Wang X, Thibodeau B, Trope M, Lin LM, Huang GT. Histologic characterization of regenerated tissues in canal space after the revitalization/revascularization procedure of immature dog teeth with apical periodontitis. J Endod. 2010;36(1):56–63.

    Article  PubMed  Google Scholar 

  17. Murray PE, Garcia-Godoy F, Hargreaves KM. Regenerative endodontics: a review of current status and a call for action. J Endod. 2007;33(4):377–90.

    Article  PubMed  Google Scholar 

  18. Huang GT, Gronthos S, Shi S. Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine. J Dent Res. 2009;88(9):792–806.

    Article  PubMed Central  PubMed  Google Scholar 

  19. Sakai VT, Cordeiro MM, Dong Z, Zhang Z, Zeitlin BD, Nor JE. Tooth slice/scaffold model of dental pulp tissue engineering. Adv Dent Res. 2011;23(3):325–32.

    Article  PubMed  Google Scholar 

  20. Prescott RS, Alsanea R, Fayad MI, Johnson BR, Wenckus CS, Hao J, et al. In vivo generation of dental pulp-like tissue by using dental pulp stem cells, a collagen scaffold, and dentin matrix protein 1 after subcutaneous transplantation in mice. J Endod. 2008;34(4):421–6.

    Article  PubMed Central  PubMed  Google Scholar 

  21. Cordeiro MM, Dong Z, Kaneko T, Zhang Z, Miyazawa M, Shi S, et al. Dental pulp tissue engineering with stem cells from exfoliated deciduous teeth. J Endod. 2008;34(8):962–9.

    Article  PubMed  Google Scholar 

  22. Sakai VT, Zhang Z, Dong Z, Neiva KG, Machado MA, Shi S, et al. SHED differentiate into functional odontoblasts and endothelium. J Dent Res. 2010;89(8):791–6.

    Article  PubMed  Google Scholar 

  23. Huang GT, Yamaza T, Shea LD, Djouad F, Kuhn NZ, Tuan RS, et al. Stem/progenitor cell-mediated de novo regeneration of dental pulp with newly deposited continuous layer of dentin in an in vivo model. Tissue Eng Part A. 2010;16(2):605–15.

    Article  PubMed Central  PubMed  Google Scholar 

  24. Rosa V, Zhang Z, Grande RH, Nor JE. Dental pulp tissue engineering in full-length human root canals. J Dent Res. 2013;92(11):970–5.

    Article  PubMed  Google Scholar 

  25. Olgart L, Kerezoudis NP. Nerve-pulp interactions. Arch Oral Biol. 1994;39(Suppl):47S–54.

    Article  PubMed  Google Scholar 

  26. Iohara K, Imabayashi K, Ishizaka R, Watanabe A, Nabekura J, Ito M, et al. Complete pulp regeneration after pulpectomy by transplantation of CD105+ stem cells with stromal cell-derived factor-1. Tissue Eng Part A. 2011;17(15–16):1911–20.

    Article  PubMed  Google Scholar 

  27. Iohara K, Zheng L, Ito M, Ishizaka R, Nakamura H, Into T, et al. Regeneration of dental pulp after pulpotomy by transplantation of CD31(-)/CD146(-) side population cells from a canine tooth. Regen Med. 2009;4(3):377–85.

    Article  PubMed  Google Scholar 

  28. Iohara K, Zheng L, Wake H, Ito M, Nabekura J, Wakita H, et al. A novel stem cell source for vasculogenesis in ischemia: subfraction of side population cells from dental pulp. Stem Cells. 2008;26(9):2408–18.

    Article  PubMed  Google Scholar 

  29. Murakami M, Horibe H, Iohara K, Hayashi Y, Osako Y, Takei Y, et al. The use of granulocyte-colony stimulating factor induced mobilization for isolation of dental pulp stem cells with high regenerative potential. Biomaterials. 2013;34(36):9036–47.

    Article  PubMed  Google Scholar 

  30. Ishizaka R, Hayashi Y, Iohara K, Sugiyama M, Murakami M, Yamamoto T, et al. Stimulation of angiogenesis, neurogenesis and regeneration by side population cells from dental pulp. Biomaterials. 2013;34(8):1888–97.

    Article  PubMed  Google Scholar 

  31. Ishizaka R, Iohara K, Murakami M, Fukuta O, Nakashima M. Regeneration of dental pulp following pulpectomy by fractionated stem/progenitor cells from bone marrow and adipose tissue. Biomaterials. 2012;33(7):2109–18.

    Article  PubMed  Google Scholar 

  32. Iohara K, Zheng L, Ito M, Tomokiyo A, Matsushita K, Nakashima M. Side population cells isolated from porcine dental pulp tissue with self-renewal and multipotency for dentinogenesis, chondrogenesis, adipogenesis, and neurogenesis. Stem Cells. 2006;24(11):2493–503.

    Article  PubMed  Google Scholar 

  33. Barry FP, Boynton RE, Haynesworth S, Murphy JM, Zaia J. The monoclonal antibody SH-2, raised against human mesenchymal stem cells, recognizes an epitope on endoglin (CD105). Biochem Biophys Res Commun. 1999;265(1):134–9.

    Article  PubMed  Google Scholar 

  34. Kastrinaki MC, Andreakou I, Charbord P, Papadaki HA. Isolation of human bone marrow mesenchymal stem cells using different membrane markers: comparison of colony/cloning efficiency, differentiation potential, and molecular profile. Tissue Eng Part C Methods. 2008;14(4):333–9.

    Article  PubMed  Google Scholar 

  35. Jarocha D, Lukasiewicz E, Majka M. Advantage of mesenchymal stem cells (MSC) expansion directly from purified bone marrow CD105+ and CD271+ cells. Folia Histochem Cytobiol. 2008;46(3):307–14.

    Article  PubMed  Google Scholar 

  36. Mafi R, Hindocha S, Mafi P, Griffin M, Khan WS. Sources of adult mesenchymal stem cells applicable for musculoskeletal applications – a systematic review of the literature. Open Orthop J. 2011;5 Suppl 2:242–8.

    Article  PubMed Central  PubMed  Google Scholar 

  37. Sugiyama M, Iohara K, Wakita H, Hattori H, Ueda M, Matsushita K, et al. Dental pulp-derived CD31(-)/CD146(-) side population stem/progenitor cells enhance recovery of focal cerebral ischemia in rats. Tissue Eng Part A. 2011;17(9–10):1303–11.

    Article  PubMed  Google Scholar 

  38. Ishizaka R, Hayashi Y, Iohara K, Sugiyama M, Murakami M, Yamamoto T, et al. Stimulation of angiogenesis, neurogenesis and regeneration by side population cells from dental pulp. Biomaterials. 2012;34(8):1888–97.

    Article  PubMed  Google Scholar 

  39. Kassis I, Vaknin-Dembinsky A, Karussis D. Bone marrow mesenchymal stem cells: agents of immunomodulation and neuroprotection. Curr Stem Cell Res Ther. 2011;6(1):63–8.

    Article  PubMed  Google Scholar 

  40. Parr AM, Tator CH, Keating A. Bone marrow-derived mesenchymal stromal cells for the repair of central nervous system injury. Bone Marrow Transplant. 2007;40(7):609–19.

    Article  PubMed  Google Scholar 

  41. Doorn J, Moll G, Le Blanc K, van Blitterswijk C, de Boer J. Therapeutic applications of mesenchymal stromal cells: paracrine effects and potential improvements. Tissue Eng Part B Rev. 2012;18(2):101–15.

    Article  PubMed  Google Scholar 

  42. Buurma B, Gu K, Rutherford RB. Transplantation of human pulpal and gingival fibroblasts attached to synthetic scaffolds. Eur J Oral Sci. 1999;107(4):282–9.

    Article  PubMed  Google Scholar 

  43. Bohl KS, Shon J, Rutherford B, Mooney DJ. Role of synthetic extracellular matrix in development of engineered dental pulp. J Biomater Sci Polym Ed. 1998;9(7):749–64.

    Article  PubMed  Google Scholar 

  44. Kim JY, Xin X, Moioli EK, Chung J, Lee CH, Chen M, et al. Regeneration of dental-pulp-like tissue by chemotaxis-induced cell homing. Tissue Eng Part A. 2010;16(10):3023–31.

    Article  PubMed Central  PubMed  Google Scholar 

  45. Schmidt A, Ladage D, Schinkothe T, Klausmann U, Ulrichs C, Klinz FJ, et al. Basic fibroblast growth factor controls migration in human mesenchymal stem cells. Stem Cells. 2006;24(7):1750–8.

    Article  PubMed  Google Scholar 

  46. Wynn RF, Hart CA, Corradi-Perini C, O’Neill L, Evans CA, Wraith JE, et al. A small proportion of mesenchymal stem cells strongly expresses functionally active CXCR4 receptor capable of promoting migration to bone marrow. Blood. 2004;104(9):2643–5.

    Article  PubMed  Google Scholar 

  47. Suzuki T, Lee CH, Chen M, Zhao W, Fu SY, Qi JJ, et al. Induced migration of dental pulp stem cells for in vivo pulp regeneration. J Dent Res. 2011;90(8):1013–8.

    Article  PubMed  Google Scholar 

  48. Kitamura C, Nishihara T, Terashita M, Tabata Y, Washio A. Local regeneration of dentin-pulp complex using controlled release of fgf-2 and naturally derived sponge-like scaffolds. Int J Dent. 2012;2012:190561.

    Article  PubMed Central  PubMed  Google Scholar 

  49. Ishimatsu H, Kitamura C, Morotomi T, Tabata Y, Nishihara T, Chen KK, et al. Formation of dentinal bridge on surface of regenerated dental pulp in dentin defects by controlled release of fibroblast growth factor-2 from gelatin hydrogels. J Endod. 2009;35(6):858–65.

    Article  PubMed  Google Scholar 

  50. Kikuchi N, Kitamura C, Morotomi T, Inuyama Y, Ishimatsu H, Tabata Y, et al. Formation of dentin-like particles in dentin defects above exposed pulp by controlled release of fibroblast growth factor 2 from gelatin hydrogels. J Endod. 2007;33(10):1198–202.

    Article  PubMed  Google Scholar 

  51. Zheng L, Amano K, Iohara K, Ito M, Imabayashi K, Into T, et al. Matrix metalloproteinase-3 accelerates wound healing following dental pulp injury. Am J Pathol. 2009;175(5):1905–14.

    Article  PubMed Central  PubMed  Google Scholar 

  52. Eba H, Murasawa Y, Iohara K, Isogai Z, Nakamura H, Nakashima M. The anti-inflammatory effects of matrix metalloproteinase-3 on irreversible pulpitis of mature erupted teeth. PLoS One. 2012;7(12):e52523.

    Article  PubMed Central  PubMed  Google Scholar 

  53. Howard C, Murray PE, Namerow KN. Dental pulp stem cell migration. J Endod. 2010;36(12):1963–6.

    Article  PubMed  Google Scholar 

  54. Pan S, Dangaria S, Gopinathan G, Yan X, Lu X, Kolokythas A, et al. SCF promotes dental pulp progenitor migration, neovascularization, and collagen remodeling – potential applications as a homing factor in dental pulp regeneration. Stem Cell Rev. 2013;9(5):655–67.

    Article  PubMed  Google Scholar 

  55. Sonoyama W, Liu Y, Yamaza T, Tuan RS, Wang S, Shi S, et al. Characterization of the apical papilla and its residing stem cells from human immature permanent teeth: a pilot study. J Endod. 2008;34(2):166–71.

    Article  PubMed Central  PubMed  Google Scholar 

  56. Sonoyama W, Liu Y, Fang D, Yamaza T, Seo BM, Zhang C, et al. Mesenchymal stem cell-mediated functional tooth regeneration in swine. PLoS One. 2006;1:e79.

    Article  PubMed Central  PubMed  Google Scholar 

  57. Huang GT, Sonoyama W, Liu Y, Liu H, Wang S, Shi S. The hidden treasure in apical papilla: the potential role in pulp/dentin regeneration and bioroot engineering. J Endod. 2008;34(6):645–51.

    Article  PubMed Central  PubMed  Google Scholar 

  58. Kaplan RN, Psaila B, Lyden D. Niche-to-niche migration of bone-marrow-derived cells. Trends Mol Med. 2007;13(2):72–81.

    Article  PubMed  Google Scholar 

  59. Chute JP. Stem cell homing. Curr Opin Hematol. 2006;13(6):399–406.

    Article  PubMed  Google Scholar 

  60. Thored P, Arvidsson A, Cacci E, Ahlenius H, Kallur T, Darsalia V, et al. Persistent production of neurons from adult brain stem cells during recovery after stroke. Stem Cells. 2006;24(3):739–47.

    Article  PubMed  Google Scholar 

  61. Robin AM, Zhang ZG, Wang L, Zhang RL, Katakowski M, Zhang L, et al. Stromal cell-derived factor 1alpha mediates neural progenitor cell motility after focal cerebral ischemia. J Cereb Blood Flow Metab. 2006;26(1):125–34.

    Article  PubMed  Google Scholar 

  62. Ohab JJ, Fleming S, Blesch A, Carmichael ST. A neurovascular niche for neurogenesis after stroke. J Neurosci. 2006;26(50):13007–16.

    Article  PubMed  Google Scholar 

  63. Kucia M, Reca R, Miekus K, Wanzeck J, Wojakowski W, Janowska-Wieczorek A, et al. Trafficking of normal stem cells and metastasis of cancer stem cells involve similar mechanisms: pivotal role of the SDF-1-CXCR4 axis. Stem Cells. 2005;23(7):879–94.

    Article  PubMed  Google Scholar 

  64. Zhou J, Shi S, Shi Y, Xie H, Chen L, He Y, et al. Role of bone marrow-derived progenitor cells in the maintenance and regeneration of dental mesenchymal tissues. J Cell Physiol. 2011;226(8):2081–90.

    Article  PubMed  Google Scholar 

  65. Galler KM, Cavender AC, Koeklue U, Suggs LJ, Schmalz G, D’Souza RN. Bioengineering of dental stem cells in a PEGylated fibrin gel. Regen Med. 2011;6(2):191–200.

    Article  PubMed  Google Scholar 

  66. Yuan Z, Nie H, Wang S, Lee CH, Li A, Fu SY, et al. Biomaterial selection for tooth regeneration. Tissue Eng Part B Rev. 2011;17(5):373–88.

    Article  PubMed Central  PubMed  Google Scholar 

  67. Hayashi Y, Yamada S, Yanagi Guchi K, Koyama Z, Ikeda T. Chitosan and fish collagen as biomaterials for regenerative medicine. Adv Food Nutr Res. 2012;65:107–20.

    Article  PubMed  Google Scholar 

  68. Galler KM, Aulisa L, Regan KR, D’Souza RN, Hartgerink JD. Self-assembling multidomain peptide hydrogels: designed susceptibility to enzymatic cleavage allows enhanced cell migration and spreading. J Am Chem Soc. 2010;132(9):3217–23.

    Article  PubMed Central  PubMed  Google Scholar 

  69. Nakashima M, Iohara K. Regeneration of dental pulp by stem cells. Adv Dent Res. 2011;23(3):313–9.

    Article  PubMed  Google Scholar 

  70. Pan HC, Wu HT, Cheng FC, Chen CH, Sheu ML, Chen CJ. Potentiation of angiogenesis and regeneration by G-CSF after sciatic nerve crush injury. Biochem Biophys Res Commun. 2009;382(1):177–82.

    Article  PubMed  Google Scholar 

  71. Pan HC, Cheng FC, Lai SZ, Yang DY, Wang YC, Lee MS. Enhanced regeneration in spinal cord injury by concomitant treatment with granulocyte colony-stimulating factor and neuronal stem cells. J Clin Neurosci. 2008;15(6):656–64.

    Article  PubMed  Google Scholar 

  72. Luo J, Zhang HT, Jiang XD, Xue S, Ke YQ. Combination of bone marrow stromal cell transplantation with mobilization by granulocyte-colony stimulating factor promotes functional recovery after spinal cord transection. Acta Neurochir (Wien). 2009;151(11):1483–92.

    Article  Google Scholar 

  73. Zhang XM, Du F, Yang D, Wang R, Yu CJ, Huang XN, et al. Granulocyte colony-stimulating factor increases the therapeutic efficacy of bone marrow mononuclear cell transplantation in cerebral ischemia in mice. BMC Neurosci. 2011;12:61.

    Article  PubMed Central  PubMed  Google Scholar 

  74. Iohara K, Murakami M, Takeuchi N, Osako Y, Ito M, Ishizaka R, et al. A novel combinatorial therapy with pulp stem cells and granulocyte colony-stimulating factor for total pulp regeneration. Stem Cells Transl Med. 2013;2(7):521–33.

    Article  PubMed Central  PubMed  Google Scholar 

  75. Allen PF, Whitworth JM. Endodontic considerations in the elderly. Gerodontology. 2004;21(4):185–94.

    Article  PubMed  Google Scholar 

  76. Kumar S, Chanda D, Ponnazhagan S. Therapeutic potential of genetically modified mesenchymal stem cells. Gene Ther. 2008;15(10):711–5.

    Article  PubMed  Google Scholar 

  77. Murray PE, Stanley HR, Matthews JB, Sloan AJ, Smith AJ. Age-related odontometric changes of human teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002;93(4):474–82.

    Article  PubMed  Google Scholar 

  78. Iida K, Takeda-Kawaguchi T, Tezuka Y, Kunisada T, Shibata T, Tezuka K. Hypoxia enhances colony formation and proliferation but inhibits differentiation of human dental pulp cells. Arch Oral Biol. 2010;55(9):648–54.

    Article  PubMed  Google Scholar 

  79. Bressan E, Ferroni L, Gardin C, Pinton P, Stellini E, Botticelli D, et al. Donor age-related biological properties of human dental pulp stem cells change in nanostructured scaffolds. PLoS One. 2012;7(11):e49146.

    Article  PubMed Central  PubMed  Google Scholar 

  80. Takeda T, Tezuka Y, Horiuchi M, Hosono K, Iida K, Hatakeyama D, et al. Characterization of dental pulp stem cells of human tooth germs. J Dent Res. 2008;87(7):676–81.

    Article  PubMed  Google Scholar 

  81. Boeuf S, Richter W. Chondrogenesis of mesenchymal stem cells: role of tissue source and inducing factors. Stem Cell Res Ther. 2010;1(4):31.

    Article  PubMed Central  PubMed  Google Scholar 

  82. Aranda P, Agirre X, Ballestar E, Andreu EJ, Roman-Gomez J, Prieto I, et al. Epigenetic signatures associated with different levels of differentiation potential in human stem cells. PLoS One. 2009;4(11):e7809.

    Article  PubMed Central  PubMed  Google Scholar 

  83. Philippe B, Luc S, Valerie PB, Jerome R, Alessandra BR, Louis C. Culture and use of mesenchymal stromal cells in Phase I and II clinical trials. Stem Cells Int. 2010;2010:503593.

    Article  PubMed Central  PubMed  Google Scholar 

  84. Noel D, Caton D, Roche S, Bony C, Lehmann S, Casteilla L, et al. Cell specific differences between human adipose-derived and mesenchymal-stromal cells despite similar differentiation potentials. Exp Cell Res. 2008;314(7):1575–84.

    Article  PubMed  Google Scholar 

  85. Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 2001;7(2):211–28.

    Article  PubMed  Google Scholar 

  86. Shi S, Robey PG, Gronthos S. Comparison of human dental pulp and bone marrow stromal stem cells by cDNA microarray analysis. Bone. 2001;29(6):532–9.

    Article  PubMed  Google Scholar 

  87. Mrozik KM, Zilm PS, Bagley CJ, Hack S, Hoffmann P, Gronthos S, et al. Proteomic characterization of mesenchymal stem cell-like populations derived from ovine periodontal ligament, dental pulp, and bone marrow: analysis of differentially expressed proteins. Stem Cells Dev. 2010;19(10):1485–99.

    Article  PubMed  Google Scholar 

  88. Phinney DG, Kopen G, Righter W, Webster S, Tremain N, Prockop DJ. Donor variation in the growth properties and osteogenic potential of human marrow stromal cells. J Cell Biochem. 1999;75(3):424–36.

    Article  PubMed  Google Scholar 

  89. Vemuri MC, Chase LG, Rao MS. Mesenchymal stem cell assays and applications. Methods Mol Biol. 2011;698:3–8.

    Article  PubMed  Google Scholar 

  90. Sensebe L, Krampera M, Schrezenmeier H, Bourin P, Giordano R. Mesenchymal stem cells for clinical application. Vox Sang. 2010;98(2):93–107.

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Dental Regenerative Medicine, Center of Advanced Medicine for Dental and Oral Diseases, National Center for Geriatrics and Gerontology, Research Institute, 35 Gengo, Morioka, Obu, 474-8522, Japan

    Misako Nakashima PhD, DDS & Koichiro Iohara DDS, PhD

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  1. Misako Nakashima PhD, DDS
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  2. Koichiro Iohara DDS, PhD
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Correspondence to Misako Nakashima PhD, DDS .

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  1. Department of Oral Biology, Institut National de la Santé et de la, Recherche Médicale, Université Paris Descartes, Paris, Paris, France

    Michel Goldberg

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Nakashima, M., Iohara, K. (2014). Experimental In Vivo Approaches of Pulp Regeneration. In: Goldberg, M. (eds) The Dental Pulp. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-55160-4_15

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