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Methods for Purification and Characterization of Microbial Phenazines

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Microbial Phenazines

Abstract

Phenazines are nitro aromatic redox-active antibiotics produced under the control of quorum sensing mechanism by a diverse range of bacterial genera with various color intensities varies from blue, green, purple, yellow, red to even brown. Production of phenazine showed (1) taxonomic value when related to few genera, (2) functional role in environment and (3) bioactive potential with respect to structure; however due to poor structural understanding its correlation is still incomplete with diversity and bioactivity. Thus isolation and identification of phenazine antibiotic is highly desirable. Current chapter resolves the methodologies for various phenazines isolation as well as its identification through different spectroscopic and electrophoretic methods in different bacterial systems.

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Abbreviations

MS:

Mass spectrometry

EI-MS:

Electron impact mass spectroscopy

HRMS:

High-resolution mass spectrometry

FI MS:

Field ionization mass spectrometry

HRESIMS:

High resolution electron spray ionization mass spectroscopy

LRCIMS:

Low resolution chemical ionization mass spectrometry

HRCIMS:

High resolution chemical ionization mass spectrometry

HR-FAB-MS:

High resolution fast atom bombardment mass spectrum

DCI-MS:

Desorption-chemical ionization mass spectrometry

LC/MS:

Liquid chromatography/mass spectrometry

GC-MS:

Gas chromatography–mass spectrometry

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Authors and Affiliations

  1. Department of Microbiology, School of Life Sciences, North Maharashtra University, Jalgaon, Maharashtra, 425 001, India

    Meghraj S. Kadam, Sandeep G. Patil, Prashant R. Dane, Madhuri K. Pawar & Sudhir B. Chincholkar

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  1. Meghraj S. Kadam
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  2. Sandeep G. Patil
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  4. Madhuri K. Pawar
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  5. Sudhir B. Chincholkar
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Correspondence to Meghraj S. Kadam .

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  1. School of Life Sciences, Department of Microbiology, North Maharashtra University, Jalgaon, Maharashtra, India

    Sudhir Chincholkar

  2. Department of Plant Pathology, Washington State University, Pullman, Washington, USA

    Linda Thomashow

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Kadam, M.S., Patil, S.G., Dane, P.R., Pawar, M.K., Chincholkar, S.B. (2013). Methods for Purification and Characterization of Microbial Phenazines. In: Chincholkar, S., Thomashow, L. (eds) Microbial Phenazines. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-40573-0_6

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