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The Mechanism of Apoptosis in Adenocarcinoma of Lung Cancer A549 Cells Induced by Albumin-Derived from Peanut

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Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 250))

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Abstract

Peanut albumin which possesses protease inhibitor activity could be used as an anticancer regent. This paper would discuss the effect and mechanism of apoptosis induced by peanut albumin on human adenocarcinoma lung cancer A549. MTT assay was used to evaluate the inhibition of peanut albumin on 11 kinds of cancer cells. Compared with other cells, there was the great significant change in A549 cells. Morphological structures were detected by fluorescence microscope, and cell cycle was measured by flow cytometry (FCM). Annexin V/PI was used to detect cell cycle of A549 treated with peanut albumin. The differential expressions between the group treated with peanut albumin and the control were detected by Western-blot and quantitative-PCR. It was found that peanut albumin could inhibit proliferation of A549 cells, and induce apoptosis of A549. The possibility apoptosis of A549 cells induced by peanut albumin maybe correlated with mitochondrial apoptosis pathway.

Note: Y. Sun and M. Long are joint primary author.

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Author information

Authors and Affiliations

  1. College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China

    Minhui Long, Yuejun Sun, Wen Chu, Yue Ma, Shenheng Luo & Aipo Diao

  2. College of Life Science, South China Normal University, Guangzhou, 510631, People’s Republic of China

    Minhui Long, Ni Chen & Zimin Zhang

  3. State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, 100071, People’s Republic of China

    Zhongpeng Zhao

Authors
  1. Minhui Long
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  2. Yuejun Sun
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  3. Ni Chen
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  4. Zimin Zhang
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  5. Wen Chu
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  6. Yue Ma
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  7. Shenheng Luo
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  8. Zhongpeng Zhao
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  9. Aipo Diao
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Corresponding authors

Correspondence to Minhui Long or Aipo Diao .

Editor information

Editors and Affiliations

  1. College of Bioengineering ,Tianjin University of Science and Technology, Tianjin, People's Republic of China

    Tong-Cun Zhang

  2. Nanjing University of Technology, Nanjing, People's Republic of China

    Pingkai Ouyang

  3. Department of Microbiology & Molecular Genetics, University of Texas- Houston Medical School, Texas, Texas, USA

    Samuel Kaplan

  4. Wellcome Trust Sanger Institute, Cambridge, United Kingdom

    Bill Skarnes

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© 2014 Springer-Verlag Berlin Heidelberg

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Long, M. et al. (2014). The Mechanism of Apoptosis in Adenocarcinoma of Lung Cancer A549 Cells Induced by Albumin-Derived from Peanut. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 250. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37922-2_68

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  • DOI: https://doi.org/10.1007/978-3-642-37922-2_68

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-37921-5

  • Online ISBN: 978-3-642-37922-2

  • eBook Packages: EngineeringEngineering (R0)

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