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Effects of Medium Components and Fermentation Conditions on Cytidine Production by Recombinant Escherichia coli CYT20

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Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 249))

Abstract

Recently, cytidine is gaining more attention as a precursor for antiviral drugs. Cytidine is produced by some microorganisms fermentation. Cytidine producing strain Escherichia coli CYT20 was bred in our previous study. Glucose, (NH4)2SO4, and temperature were selected as variables for optimization of fermentation medium and conditions for cytidine production. Among ingredients, different concentrations of initial glucose and (NH4)2SO4 had positive effects on cytidine production, and pH 7.0 and temperature 36 °C were favoured fermentation conditions for cytidine production by E. coli CYT20. After shake flask batch fermentation, production of cytidine was measured by high performance liquid chromatography. The results showed that it was 1,866 mg/l under optimized condition and it was 1,115 mg/l under un-optimized condition, cytidine production was increased by 67.4 %.

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References

  1. Asahi S, Tsunem Y (1994) Cytidine production by mutants of Bacillus subtilis. Biosci Biotech Biochem 58(8):1399–1402

    Article  CAS  Google Scholar 

  2. Headrick JP, Ely SW, Matherne GP (1993) Methods for cytidine determination. Am J Physiol 264(1):31

    Google Scholar 

  3. Switzer RL (2009) Discoveries in bacterial nucleotide metabolism. J Biol Chem 284(11):6585–6594

    Article  CAS  Google Scholar 

  4. Zhu J, Thakker C, San K-Y, Bennett G (2011) Effect of culture operating conditions on succinate production in a multiphase fed-batch bioreactor using an engineered Escherichia coli strain. Appl Microbiol Biotechnol 92(3):499–508

    Article  CAS  Google Scholar 

  5. Wang J, Zhu J, Bennett GN, San K-Y (2011) Succinate production from different carbon sources under anaerobic conditions by metabolic engineered Escherichia coli strains. Metab Eng 13(3):328–335

    Article  CAS  Google Scholar 

  6. Lin HY, Neubaur P (2000) Influence of controlled glucose oscillations on a fed-batch process of recombinant Escherichia coli. Biotechnology 79:27–37

    CAS  Google Scholar 

  7. Rahmen N, Kunze M, Büchs J (2011) Influence of recombinant protein production on respiration activity of E. coli shake flask cultures in auto induction media. Osaka Mini Symposium, Osaka University

    Google Scholar 

  8. Tramper J, Asahi S (1989) Methods for production of cytidine and deoxycytidine. Patent 4839285

    Google Scholar 

  9. Tyge G, Thomas A (2003) High-temperature liquid chromatography. J Chromtogr A 1000(12):743–755

    Google Scholar 

  10. Park KH, RubinL EG (1992) Research on determination of cytidine by reversed phase chromatography. Cric Lation Res 71(4):992

    CAS  Google Scholar 

  11. Carneiro S, Villas-Bôas SG, Eugénio C, Ferreira EC, Rocha I (2011) Metabolic footprint analysis of recombinant Escherichia coli strains during fed-batch fermentations. Mol BioSyst 7:899–910

    CAS  Google Scholar 

  12. Xiufu H, Daidi F, Yane L (2006) Kinetics of high cell density fed-batch culture of recombinant Escherichia coli producing human-like collagen. Chin J Chem Eng 14:242–247

    Article  Google Scholar 

  13. Lennox B, Montague GA, Hiden HG, Kornfeld G, Goulding PR (2001) Process monitoring of an industrial fed-batch fermentation. Biotechnol Bioeng 74(2):125–135

    Google Scholar 

  14. Bakonyi P, Nemestóthy N, Lövitusz É, Bélafi-Bakó K (2011) Application of Plackett- Burman experimental design to optimize biohydrogen fermentation by E. coli (XL1-BLUE). Int J Hydrogen Energy 36(21):13949–13954

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We thank colleagues for critical reading of the manuscript and providing valuable suggestions. This work was supported by the Research Program of Tianjin University of Science and Technology (20100211), by Program for Changjiang Scholars and Innovative Research Team in University (IRT 1166), and by the Research Program of University in Ningxia (2012).

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Authors and Affiliations

  1. College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic China

    Haitian Fang, Xixian Xie, Qingyang Xu, Chenglin Zhang & Ning Chen

  2. College of Agriculture, Ningxia University, Yinchuan, 750021, People’s Republic China

    Haitian Fang

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  1. Haitian Fang
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  2. Xixian Xie
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  3. Qingyang Xu
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  4. Chenglin Zhang
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  5. Ning Chen
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Corresponding author

Correspondence to Ning Chen .

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Editors and Affiliations

  1. College of Bioengineering, Tianjin University of Science and Technology, Tianjin, People's Republic of China

    Tong-Cun Zhang

  2. Nanjing University of Technology, Nanjing, People's Republic of China

    Pingkai Ouyang

  3. Department of Microbiology & Molecular Genetics, University of Texas Houston Medical School, Texas, Texas, USA

    Samuel Kaplan

  4. Wellcome Trust Sanger Institute, Cambridge, United Kingdom

    Bill Skarnes

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Fang, H., Xie, X., Xu, Q., Zhang, C., Chen, N. (2014). Effects of Medium Components and Fermentation Conditions on Cytidine Production by Recombinant Escherichia coli CYT20. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 249. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37916-1_2

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  • DOI: https://doi.org/10.1007/978-3-642-37916-1_2

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-37915-4

  • Online ISBN: 978-3-642-37916-1

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