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Zellkultur im großen Maßstab, Automatisierung

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Zell- und Gewebekultur

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Zusammenfassung

Auch im Labor ist es hin und wieder nötig, entweder größere Mengen von Zellen (Biomasse) oder Produkte dieser Zellen (z.B. monoklonale Antikörper, Abschn. 19.3; rekombinante Proteine, Abschn. 18.4) zu gewinnen (Massenzellkultur, engl. Scale- up). Es gibt sehr verschiedene Methoden, größere Zellmengen zu züchten, je nachdem, ob es sich um adhärente Zellen (Monolayer) oder in Suspension wachsende Zellen (z. B. lymphoblastoide Zellen, bestimmte Hybridomakulturen, adaptierte CHO-Zellen, Sf9-Kulturen) handelt (Abb. 22-1).

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Authors and Affiliations

  1. Sektion für Physiologie, Medizinische Universität Innsbruck, Fritz-Pregl-Str. 3, A-6020, Innsbruck, Germany

    Prof. Dr. Gerhard Gstraunthaler

  2. Institut für angewandte Zellkultur, Balanstr. 6, D-81669, München, Germany

    Prof. Dr. Toni Lindl

Authors
  1. Prof. Dr. Gerhard Gstraunthaler
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  2. Prof. Dr. Toni Lindl
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Gstraunthaler, G., Lindl, T. (2013). Zellkultur im großen Maßstab, Automatisierung. In: Zell- und Gewebekultur. Springer Spektrum, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-35997-2_22

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