Abstract
The molecular mechanisms underlying plant sensitivity to acid stress are still unclear. Therefore, we intend to elucidate the mechanism of acid stress acclimation. The sll1558 gene in Synechocystis sp. PCC 6803 was identified as an up-regulated gene by DNA microarray analysis in a short-time acid treatment (Ohta et al., 2005). This gene encodes mannose-1-phosphate guanylyltransferase, which catalyzes the reaction to GDP-D-mannose involved in N-glycan biosynthesis. The sll1496 gene encodes the same enzyme. In this study, deletion mutants of these genes were constructed and phenotypes were analyzed. Both genes were found to be dispensable under normal growth conditions at pH 8.0. However, the sll1558 deletion mutant was highly sensitive to acid stress conditions at pH 6.0. In contrast, the sll1496 gene was found to be dispensable, as the sll1496 deletion mutant was slightly sensitive to acid stress. Furthermore, the At2g39770 (cyt1) gene in Arabidopsis thaliana has an orthologous relationship with sll1 558. The cyt1 mutant was more sensitive to acid stress conditions when compared with wild-type (WT) cells in the roots. The results of this study indicate that N-glycan biosynthesis contributes to acid stress and that only the sll1558 gene plays an important role in acid stress in Synechocystis.
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© 2013 Zhejiang University Press, Hangzhou and Springer-Verlag Berlin Heidelberg
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Sambe, M., Kitayama, S., Moriyama, A., Uchiyama, J., Ohta, H. (2013). The Role of sll1558 and sll1496 Genes under Acid Stress Conditions in the Cyanobacterium Synechocystis sp. PCC 6803. In: Photosynthesis Research for Food, Fuel and the Future. Advanced Topics in Science and Technology in China. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-32034-7_124
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DOI: https://doi.org/10.1007/978-3-642-32034-7_124
Publisher Name: Springer, Berlin, Heidelberg
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