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Chromosomal Fluorescence In Situ Hybridization

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  • First Online:
Encyclopedia of Cancer

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Synonyms

FISH

Definition

Detection of specific chromosome structures by hybridization of fluorescence dye-conjugated probes to DNA. The FISH technique relies on the hybridization of DNA probes which identify specific chromosomal structures. Probes can be used which are specific for the centromere region of particular chromosomes, for genes, or for complete chromosomes. The DNA of both the applied probe and of the patient sample are denaturated, i.e., both DNA strands of the double helix are separated. During the following renaturation, the DNA probes attach to the complementary section of the patient’s DNA (hybridization). The DNA probes are either directly conjugated to a fluorescent dye or are analyzed using fluorescence conjugated antibodies. The respective chromosome structures therefore are assessable as fluorescence signals.

A significant advantage of the method lies in its applicability not only to metaphases but also to interphase nuclei. A disadvantage is that information is...

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See Also

  • (2012) Centromere. In: Schwab M (ed) Encyclopedia of cancer, 3rd edn. Springer, Berlin/Heidelberg, p 744. doi: 10.1007/978-3-642-16483-5_1028

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  • (2012) Chromosome. In: Schwab M (ed) Encyclopedia of cancer, 3rd edn. Springer, Berlin/ Heidelberg, p 848. doi: 10.1007/978-3-642-16483-5_1145

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  • (2012) FISH. In: Schwab M (ed) Encyclopedia of cancer, 3rd edn. Springer, Berlin/Heidelberg, pp 1415–1416. doi: 10.1007/978-3-642-16483-5_2197

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  • (2012) Fluorescence in situ hybridisation. In: Schwab M (ed) Encyclopedia of cancer, 3rd edn. Springer, Berlin/Heidelberg, p 1436. doi: 10.1007/978-3-642-16483-5_6740

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  • (2012) PCR. In: Schwab M (ed) Encyclopedia of cancer, 3rd edn. Springer, Berlin/Heidelberg, p 2803. doi: 10.1007/978-3-642-16483-5_4417

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Authors and Affiliations

  1. German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120, Heidelberg, Germany

    Manfred Schwab

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  1. Manfred Schwab
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Correspondence to Manfred Schwab .

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Editors and Affiliations

  1. Abt. Tumorgenetik, Deutsches Krebsforschungszentrum, Heidelberg, Germany

    Manfred Schwab

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© 2015 Springer-Verlag Berlin Heidelberg

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Schwab, M. (2015). Chromosomal Fluorescence In Situ Hybridization. In: Schwab, M. (eds) Encyclopedia of Cancer. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27841-9_2218-2

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  • DOI: https://doi.org/10.1007/978-3-642-27841-9_2218-2

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Online ISBN: 978-3-642-27841-9

  • eBook Packages: Springer Reference Biomedicine and Life SciencesReference Module Biomedical and Life Sciences

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