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Popliteal Lymph Node Assay

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Encyclopedia of Immunotoxicology

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Synonyms

PLNA

Short Description

The popliteal lymph node assay (PLNA) is in use to test the immunostimulatory capacity of low molecular weight compounds, in particular pharmaceuticals. Briefly, compounds are injected subcutaneously into the hind footpad of either mice or rats, and 6–8 days later the draining popliteal lymph node (PLN) is excised and compared with popliteal lymph nodes from vehicle-treated animals. Comparison is basically made between the weight and the cellularity of the nodes; the ratio of these parameters from compound-treated over vehicle-treated PLNs (designated the PLN index) indicates whether or not the compound is immunostimulatory. Immunostimulation is regarded as one of the possible prerequisites for pharmaceuticals and other compounds to activate and sensitize T cells and to induce systemic allergy or autoimmune-like derangements.

Characteristics

In the late 1970s and early 1890s, Gleichmann and colleagues realized that graft-versus-host (GvH) reactions might...

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References

  • Gleichmann H (1981) Studies on the mechanism of drug sensitization: T-cell-dependent popliteal lymph node reaction to diphenylhydantoin. Clin Immunol Immunopathol 18:203–211

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  • Gleichmann E, Pal ST, Rolink AG, Radasziewicz T, Gleichmann H (1984) Graft versus host reactions: clues to the etiopathology of a spectrum of immunological diseases. Immunol Today 5:324–332

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  • Kammüller ME, Thomas C, De Bakker JM, Bloksma N, Seinen W (1989) The popliteal lymph node assay in mice to screen for the immune dysregulating potential of chemicals-a preliminary study. Int J Immunopharmacol 11:293–300

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  • Pieters R, Albers R (1999) Screening tests for autoimmune-related immunotoxicity. Environ Health Perspect 107:673–677

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  • Verdier F, Virat M, Descotes J (1990) Applicability of the popliteal lymph node assay in the Brown Norway rat. Immunopharmacol Immunotoxicol 12:669–677

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Author information

Authors and Affiliations

  1. Head Immunotoxicology, Institute for Risk Assessment Sciences (IRAS), Yalelaan 104, 80176, 3508 TD, Utrecht, The Netherlands

    Raymond Pieters

Authors
  1. Raymond Pieters
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Corresponding author

Correspondence to Raymond Pieters .

Editor information

Editors and Affiliations

  1. Pharmaforschungszentrum, Bayer Health Care AG, Wuppertal, Nordrhein-Westfalen, Germany

    Hans-Werner Vohr

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© 2014 Springer-Verlag Berlin Heidelberg

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Pieters, R. (2014). Popliteal Lymph Node Assay. In: Vohr, HW. (eds) Encyclopedia of Immunotoxicology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27786-3_1200-2

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  • DOI: https://doi.org/10.1007/978-3-642-27786-3_1200-2

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Online ISBN: 978-3-642-27786-3

  • eBook Packages: Springer Reference Biomedicine and Life SciencesReference Module Biomedical and Life Sciences

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