Abstract
In our research we studied gene expression in primary cell cultures cultured from affected palmar fascia in comparison with patient matched nonaffected fascia by use of Affymetrix whole-genome microarray platform. Differentially expressed genes identified in our study might be responsible for pathological changes and rise of typical Dupuytren’s contracture symptoms. RT-PCR method has been employed for validation of microarray results. Expression of ten genes correlated with the obtained microarray data. Especially, deregulation of six genes involved in signal transduction pathways that might be responsible for changes in the extracellular matrix and development of palmar contracture have been observed. Our results complement current knowledge on gene expression patterns of Dupuytren’s contracture.
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Acknowledgments
We acknowledge Croatian Ministry of Science, Education and Sport (grants: 335-0000000-3532 and 335-0982464-2393) and Professor Kresimir Pavelic, Ph.D., M.D., Head of the Department of Biotechnology, University of Rijeka, Croatia.
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Pavelic, S.K., Ratkaj, I. (2012). Microarray Expression Analysis of Primary Dupuytren’s Contracture Cells. In: Eaton, C., Seegenschmiedt, M., Bayat, A., Gabbiani, G., Werker, P., Wach, W. (eds) Dupuytren’s Disease and Related Hyperproliferative Disorders. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-22697-7_14
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DOI: https://doi.org/10.1007/978-3-642-22697-7_14
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