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Bioproduction of trans-Resveratrol from Grapevine Cell Cultures

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Abstract

The use of grapevine cell cultures to increase trans-resveratrol and stilbene-related production under controlled conditions is viewed as a promising biotechnological alternative to their extraction from the whole plant or their chemical synthesis. At present, in vitro plant cultures are rarely used as a commercial method for the production of bioactive compounds. The major problems hindering the development of the large-scale culture of plant cells include low productivity, cell line instability, and the difficulties involved in scaling up. This chapter describes how the composition of the medium, culture conditions, elicitors, bioreactor design, and other critical parameters influence the behavior of grapevine cell cultures and how optimization of these factors could allow improvements in trans-resveratrol and stilbene-related production. For these reasons, this chapter describes how high-producing Vitis cell lines, nutrient and precursor feeding, elicitation with stress factors, and signaling molecules, as well as metabolic engineering can be used to increase the production of these secondary metabolites.

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Abbreviations

4CL:

4-coumarate-CoA ligase

araE:

The low-affinity high-capacity bacterial araE transporter

C4H:

Cinnamate 4-hydroxylase

CHS:

Chalcone synthase

cv:

Cultivar

DW:

Dry cell weight

ET:

Ethylene

FW:

Fresh cell weight

JA:

Jasmonic acid

Phe:

Phenylalanine

MeJa:

Methyl jasmonate

PAL:

Phenylalanine ammonia-lyase

SA:

Salicylic acid

STS:

Stilbene synthase

trans-R:

trans-Resveratrol

3,4′,5:

Trihydroxystilbene

UV:

Ultraviolet

Vsts :

Vitis stilbene synthase

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Acknowledgments

This study was supported by the Fundación Séneca, Agencia de Ciencia y Tecnología de la Región de Murcia en el marco de II PCTRM 2007-10 (08799/PI/08), and MICINN-FEDER (BIO2008-2941 and BIO2011-29856-C02-02). Almagro L. and Sabater-Jara AB held a grant from the Ministerio de Ciencia e Innovación.

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Authors and Affiliations

  1. Department of Plant Biology, Faculty of Biology, University of Murcia, Campus Universitario de Espinardo, 30100, Murcia, Spain

    Dr. Lorena Almagro (Faculty of Biology) & Dr. María Angeles Pedreño (Faculty of Biology)

  2. Department of Agrochemistry and Biochemistry, University of Alicante, Campus Universitario de San Vicente de Raspeig, Alicante, 03080, Spain

    Juan Carlos Vera-Urbina (Faculty of Biology), Susana Sellés-Marchart (Faculty of Biology) & Roque Bru

  3. Department of Plant Biology, University of Murcia, Campus Universitario de Espinardo, 30100, Murcia, Spain

    Dr. Sarai Belchí-Navarro (Faculty of Biology) & Ana Belén Sabater-Jara (Faculty of Biology)

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  1. Dr. Lorena Almagro
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  2. Dr. Sarai Belchí-Navarro
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  3. Ana Belén Sabater-Jara
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  4. Juan Carlos Vera-Urbina
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  5. Susana Sellés-Marchart
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  7. Dr. María Angeles Pedreño
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Editors and Affiliations

  1. Botany Department, M.L. Sukhadia University, Durga Nursery Road, Udaipur, 313001, Rajasthan, India

    Kishan Gopal Ramawat

  2. Institute of Vine and Wine Sciences, Biological-Active Plant Substances Study, University of Bordeaux, 210 Chemin de Leysotte CS 50008, Villenave d'Ornon, 33882, France

    Jean-Michel Mérillon

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Almagro, L. et al. (2013). Bioproduction of trans-Resveratrol from Grapevine Cell Cultures. In: Ramawat, K., Mérillon, JM. (eds) Natural Products. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-22144-6_54

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