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One Alternative to Germ Cells Cryopreservation: Cryobanking of Somatic Cells in Sturgeon

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Biology and Conservation of the European SturgeonAcipenser sturioL. 1758

Abstract

Cryobanking of somatic cells is one option for preserving both parental genomes when gametes are not available or are unsuitable for cryopreservation. Somatic cells can be obtained from skin or fin biopsies, which are easy to collect long before sexual maturation is reached. This chapter develops a strategy to be considered for cryobanking of Acipenser sturio somatic cells, with the example of fin as a cell reservoir. The collected fins should be appropriately stored to allow shipping to laboratory facilities where they will be either frozen as a whole, or cultured to grow out cells which will be cryopreserved thereafter. Fish reconstruction from somatic cells will require the use of nuclear transfer technology, where the nucleus of the cryopreserved cell is transferred into the enucleated oocyte of a closely related species. The promises and drawbacks of this reconstruction technology are developed.

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Acknowledgements

The study on Acipenser sturio fin culture and cryopreservation was funded by the INRA AIP CRB-Bioressources.

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Authors and Affiliations

  1. INRA, UR1037 SCRIBE, Cryopreservation and Regeneration, F-35000, Rennes, France

    Catherine Labbe, Alexandra Depince & Pierre-Yves Le Bail

  2. Sturgeon consultant, 4, Rue du pas de madame, 33980, Audenge, France

    Patrick Williot

Authors
  1. Catherine Labbe
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  2. Alexandra Depince
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  3. Pierre-Yves Le Bail
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  4. Patrick Williot
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Corresponding author

Correspondence to Catherine Labbe .

Editor information

Editors and Affiliations

  1. rue du Pas de Madame 4, Audenge, 33980, France

    Patrick Williot

  2. UR Ecosystemes Estuariens et Poissons Mi, Cemagref, Avenue de Verdun 50, Cestas cedex, 33612, France

    Eric Rochard

  3. CÉPAM, Labo. Archéologie, CNRS Valbonne, avenue Albert-Einstein 250, Valbonne, 06560, France

    Nathalie Desse-Berset

  4. Abt. Biologie und Ökologie der Fische, Humboldt-Universität Berlin, Philippstr. 13, Berlin, 10115, Germany

    Frank Kirschbaum

  5. Gewässerökologie und Binnenfischerei, Leibniz-Institut für, Müggelseedamm 310, Berlin, 12587, Berlin, Germany

    Jörn Gessner

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Labbe, C., Depince, A., Le Bail, PY., Williot, P. (2011). One Alternative to Germ Cells Cryopreservation: Cryobanking of Somatic Cells in Sturgeon. In: Williot, P., Rochard, E., Desse-Berset, N., Kirschbaum, F., Gessner, J. (eds) Biology and Conservation of the European SturgeonAcipenser sturioL. 1758. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-20611-5_47

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