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Artificial Zinc Finger Peptides: A Promising Tool in Biotechnology and Medicine

  • Chapter
Transcription Factors

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 166))

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Abstract

Since their first description about two decades ago, zinc finger DNA-binding proteins have constantly stimulated the creativity of scientists. In particular, the high versatility and modularity of the zinc finger domains make them optimal building blocks for constructing artificial transcription factors potentially able to control the expression of any desired gene in cells and organisms. Disease-related genes can be either turned off or enhanced for the purpose of treating conditions such as genetic disease, cancer or viral infection. Alternatively, the expression of beneficial genes can be boosted to generate animals or plants with advantageous characteristics. Moreover, zinc finger peptides can further expand the number of possible applications on the basis of their peculiar capability to specifically bind RNA. The opportunity to re-program the expression of specific genes at will represents a powerful tool in basic science, biotechnology and molecular medicine. In this chapter, we will focus on the different approaches followed to design and to deliver artificial zinc finger transcription factors with the final aim of approaching functional genomics, phenotypic engineering and human gene therapy.

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  1. CNA, Istituto Biologia e Patologia Molecolari (IBPM), Viale Marx 43, 00137, Rome, Italy

    N. Corbi, V. Libri & C. Passananti

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  1. N. Corbi
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  2. V. Libri
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Editors and Affiliations

  1. Max-Delbrück-Centrum für Molekulare Medizin, Robert-Rössle-Strasse 10, 13125, Berlin, Germany

    Manfred Gossen

  2. atugen AG, Robert-Rössle-Strasse 10, 13125, Berlin, Germany

    Jörg Kaufmann

  3. Department of Biochemistry and Molecular Biology, Michigan State University, 510 Biochemistry Building, East Lansing, MI, 48824-1319, USA

    Steven J. Triezenberg (Associate Director) (Associate Director)

  4. Graduate Program in Cell and Molecular Biology, Michigan State University, 510 Biochemistry Building, East Lansing, MI, 48824-1319, USA

    Steven J. Triezenberg (Associate Director) (Associate Director)

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Corbi, N., Libri, V., Passananti, C. (2004). Artificial Zinc Finger Peptides: A Promising Tool in Biotechnology and Medicine. In: Gossen, M., Kaufmann, J., Triezenberg, S.J. (eds) Transcription Factors. Handbook of Experimental Pharmacology, vol 166. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-18932-6_16

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  • DOI: https://doi.org/10.1007/978-3-642-18932-6_16

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