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Bleomycin-Oligonucleotide Conjugates as Site-Specific Nucleases

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Artificial Nucleases

Part of the book series: Nucleic Acids and Molecular Biology ((NUCLEIC,volume 13))

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Abstract

The idea of applying reactive oligonucleotide derivatives on nucleic acids (NA) for the purpose of achieving specific reactive effects was already suggested more than 30 years ago (Belikova et al. 1967). Since then, various oligonucleotide derivatives have been created which are capable of damaging nucleic acids and thereby influencing their function (Knorre et al. 1994). However, such oligonucleotide conjugates have not yet been applied as artificial nucleases in either scientific or medical practice. A number of drawbacks hampering a wide application of these oligonucleotide derivatives have been revealed and not yet overcome. One of these drawbacks is insufficient interaction efficiency with a target NA; another, a low selectivity of this interaction.

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  1. Novosibirsk Institute of Bioorganic Chemistry, 8 Lavrentyev Ave., Novosibirsk, 630090, Russia

    P. E. Vorobjev & V. F. Zarytova

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  1. P. E. Vorobjev
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  2. V. F. Zarytova
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  1. Novosibirsk Institute of Bioorganic Chemistry, 8, Lavrentiev ave., 630090, Novosibirsk, Russia

    Marina A. Zenkova

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Vorobjev, P.E., Zarytova, V.F. (2004). Bleomycin-Oligonucleotide Conjugates as Site-Specific Nucleases. In: Zenkova, M.A. (eds) Artificial Nucleases. Nucleic Acids and Molecular Biology, vol 13. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-18510-6_15

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  • DOI: https://doi.org/10.1007/978-3-642-18510-6_15

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-62139-0

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