Abstract
Holography is an attractive imaging technique as it offers the ability to view a complete three-dimensional volume from one image. However, holography is not widely applied to the regime of fluorescence microscopy, because fluorescent light is incoherent and creating holograms requires a coherent interferometer system. We review two methods of generating digital Fresnel holograms of three-dimensional microscopic specimens illuminated by incoherent light. In the first method, a scanning hologram is generated by a unique scanning system in which Fresnel zone plates (FZP) are created by a coherently illuminated interferometer. In each scanning period, the system produces an on-axis Fresnel hologram. The twin image problem is solved by a linear combination of at least three holograms taken with three FZPs with different phase values. The second hologram reviewed here is the Fresnel incoherent correlation hologram. In this motionless holographic technique, light is reflected from the 3-D specimen, propagates through a spatial light modulator (SLM), and is recorded by a digital camera. Three holograms are recorded sequentially, each for a different phase factor of the SLM function. The three holograms are superposed in the computer, such that the result is a complex-valued Fresnel hologram that does not contain a twin image. When these two types of hologram are reconstructed in the computer, the 3-D properties of the specimen are revealed.
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References
A.W. Lohmann, Wavefront reconstruction for incoherent objects. J. Opt. Soc. Am. 55, 1555–1556 (1965)
G.W. Stroke, R.C. Restrick, III, Holography with spatially noncoherent light. Appl. Phys. Lett. 7, 229–231 (1965)
G. Cochran, New method of making Fresnel transforms with incoherent light. J. Opt. Soc. Am. 56, 1513–1517 (1966)
P. Peters, Incoherent holograms with a mercury light source. J. Appl. Phys. Lett. 8, 209–210 (1966)
H.R. Worthington, Jr., Production of holograms with incoherent illumination. J. Opt. Soc. Am. 56, 1397–1398 (1966)
J.B. Breckinridge, Two-dimensional white light coherence interferometer. Appl. Opt.13, 2760–2762 (1974)
A.S. Marathay, Noncoherent-object hologram: Its reconstruction and optical processing. J. Opt. Soc. Am. A 4, 1861–1868 (1987)
L.M. Mugnier, G.Y. Sirat, D. Charlot, Conoscopic holography: Two-dimensional numerical reconstructions. Opt. Lett. 18, 66–68 (1993)
Y. Li, D. Abookasis, J. Rosen, Computer-generated holograms of three-dimensional realistic objects recorded without wave interference. Appl. Opt. 40, 2864–2870 (2001)
Y. Sando, M. Itoh, T. Yatagai, Holographic three-dimensional display synthesized from three-dimensional Fourier spectra of real-existing objects. Opt. Lett. 28, 2518–2520 (2003)
N.T Shaked, J. Rosen, Multiple-viewpoint projection holograms synthesized by spatially incoherent correlation with broadband functions. J. Opt. Soc. Am. A 25, 2129–2138 (2008)
J.-H. Park, M.-S. Kim, G. Baasantseren, N. Kim, Fresnel and Fourier hologram generation using orthographic projection images. Opt. Exp.17, 6320–6334 (2009)
T.-C. Poon, A. Korpel, Optical transfer function of anacousto-optic heterodyning image processor. Opt. Lett. 4, 317–319 (1979)
B.W. Schilling, T.-C. Poon, G. Indebetouw, B. Storrie, K. Shinoda,Y. Suzuki, M.H. Wu, Three-dimensional holographic fluorescence microscopy. Opt. Lett. 22, 1506–1508 (1997)
T.-C. Poon, Optical Scanning Holography with MATLAB (Springer, New York, NY, 2007)
T.-C. Poon, Recent progress in optical scanning holography. J. Hologr. Speckle 1, 6–25 (2004)
J. Rosen, G. Indebetouw, G. Brooker, Homodyne scanning holography. Opt. Exp. 14, 4280–4285 (2006)
J. Rosen, G. Brooker, Digital spatially incoherent Fresnel holography. Opt. Lett. 32, 912–914 (2007)
J. Rosen, G. Brooker, Fluorescence incoherent color holography. Opt. Exp. 15, 2244–2250 (2007)
J. Rosen, G. Brooker, Non-scanning motionless fluorescence three-dimensional holographic microscopy. Nat. Photon. 2, 190–195 (2008)
B. Katz, J. Rosen, Super-resolution in incoherent optical imaging using synthetic aperture with Fresnel elements. Opt. Exp. 18, 962–972 (2010)
J.W. Goodman, Introduction to Fourier Optics, 2nd edn. (McGraw-Hill, New York, NY, 1996)
S.M. Beck, J.R. Buck, W.F. Buell, R.P. Dickinson, D.A. Kozlowski, N.J. Marechal, T.J. Wright, Synthetic-aperture imaging laser radar: Laboratory demonstration and signal processing. Appl. Opt. 44, 7621–7629 (2005)
V. Mico, Z. Zalevsky, P. GarcÃa-MartÃnez, J. GarcÃa, Synthetic aperture superresolution with multiple off-axis holograms. J. Opt. Soc. Am. A 23, 3162–3170 (2006)
L. MartÃnez-Lón, B. Javidi, Synthetic aperture single-exposure on-axis digital holography. Opt. Exp.16, 161–169 (2008)
G. Indebetouw, Y. Tada, J. Rosen, G. Brooker, Scanning holographic microscopy with resolution exceeding the Rayleigh limit of the objective by superposition of off-axis holograms. Appl. Opt.46, 993–1000 (2007)
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Rosen, J., Brooker, G. (2011). Incoherent Digital Holographic Microscopy with Coherent and Incoherent Light. In: Ferraro, P., Wax, A., Zalevsky, Z. (eds) Coherent Light Microscopy. Springer Series in Surface Sciences, vol 46. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-15813-1_4
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DOI: https://doi.org/10.1007/978-3-642-15813-1_4
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