Abstract
Sponge miR-Mask technology combines the principle of actions of miRNA Sponge and miR-Mask technologies. Like a miR-Mask but unlike a miRNA Sponge, a Sponge miR-Mask does not directly interact with a miRNA but is designed to bind to the binding site of a miRNA seed family in the 3′UTR of all target mRNAs; like a miRNA Sponge but unlike a miR-Mask, a Sponge miR-Mask binds by a partial complementary mechanism only with its seed site 8 nts base-pairing to its target genes. In this way, a Sponge miR-Mask is able to block access of all members of a miRNA seed family of interest to their binding sites or a particular miRNA to their multiple binding sites in a gene to target the actions of all members of that miRNA seed family, leading to derepression of the proteins from the miRNA seed family. This technology was established by my laboratory in 2008 (unpublished observations). The Sponge miR-Mask technology belongs to the “targeting-miRNA” and “miRNA-loss-of-function” strategy. The miRNA Sponge technology complies with the ‘miRNA Seed Family’ concept (see Sect. 2.1.4 for detail).
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Wang, Z. (2009). Sponge miR-Mask Technology. In: MicroRNA Interference Technologies. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-00489-6_11
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DOI: https://doi.org/10.1007/978-3-642-00489-6_11
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