Abstract
Direct perfusion of 3D tissue engineered constructs is known to enhance osteogenesis, which can be partly attributed to enhanced nutrient and waste transport. In addition flow mediated shear stresses are known to upregulate osteogenic differentiation and mineralization. A quantification of the hydrodynamic environment is therefore crucial to interpret and compare results of in vitro bioreactor experiments. In this study a 3D CFD model for the creeping perfusion flow inside two irregular bone scaffold structures is developed, simulating the velocity field including shear stress distribution. εCT imaging techniques were used to reconstruct the geometry of both a titanium and a hydroxyapatite scaffold, starting from 430 images with a resolution of 8 µm. The resulting CFD models are built with the 3D unstructured mesher TGrid and solved with the finite volume code Fluent (ANSYS, Inc.). With a flow rate of 0.04ml/min we obtained average wall shear stresses (WSS) of 1.46mPa for the hydroxyapatite scaffold compared to 1.95mPa for the Titanium scaffold. Influence of boundary conditions and scaffold micro architecture heterogeneity has been investigated. This methodology allows to get more insight in the complex concept of tissue engineering and will likely help to understand and eventually improve the fluidmechanical aspects.
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References
Einhorn, T.A., Clinically applied models of bone regeneration in tissue engineering research. Clinical Orthopaedics and Related Research, 1999(367): p. S59–S67.
Griffith, C.K., et al., Diffusion limits of an in vitro thick prevascularized tissue. Tissue Engineering, 2005. 11(1–2): p. 257–266.
Glowacki, J., S. Mizuno, and J.S. Greenberger, Perfusion enhances functions of bone marrow stromal cells in three-dimensional culture. Cell Transplantation, 1998. 7(3): p. 319–326.
Bancroft, G.N., et al., Fluid flow increases mineralized matrix deposition in 3D perfusion culture of marrow stromal osteloblasts in a dosedependent manner. Proceedings of the National Academy of Sciences of the United States of America, 2002. 99(20): p. 12600–12605.
McGarry, J.G., et al., A comparison of strain and fluid shear stress in stimulating bone cell responses-a computational and experimental study. Faseb Journal, 2004. 18(15): p. 482-+.
Raimondi, M.T., et al. Integration of computational and experimental methods in the study of cartilage mechanobiology. 2002.
Cartmell, S.H., et al., Effects of medium perfusion rate on cell-seeded three-dimensional bone constructs in vitro. Tissue Engineering, 2003. 9(6): p. 1197–1203.
Raimondi, M.T., et al., The effect of hydrodynamic shear on 3D engineered chondrocyte systems subject to direct perfusion. Biorheology, 2006. 43(3–4): p. 215–222.
Cioffi, M., et al., Modeling evaluation of the fluid-dynamic microenvironment in tissueengineered constructs: A micro-CT based model. Biotechnology and Bioengineering, 2006. 93(3): p. 500–510.
Porter, B., et al., 3-D computational modelling of media flow through scaffolds in a perfusion bioreactor. Journal of Biomechanics, 2005. 38(3): p. 543–549.
Boschetti, F., et al., Prediction of the microfluid dynamic environment imposed to threedimensional engineered cell systems in bioreactors. Journal of Biomechanics, 2006. 39(3): p. 418–425.
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© 2009 Springer-Verlag Berlin Heidelberg
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Van Ransbeeck, P., Maes, F., Impens, S., Van Oosterwyck, H., Verdonck, P. (2009). Numerical Modeling of Perfusion Flow in Irregular Scaffolds. In: Vander Sloten, J., Verdonck, P., Nyssen, M., Haueisen, J. (eds) 4th European Conference of the International Federation for Medical and Biological Engineering. IFMBE Proceedings, vol 22. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-89208-3_642
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DOI: https://doi.org/10.1007/978-3-540-89208-3_642
Publisher Name: Springer, Berlin, Heidelberg
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