Acetic acid bacteria are strictly aerobic, acidophilic organisms that are famous for their rapid incomplete oxidations. They are the elicitors of various wine faults, mainly the formation of vinegar taste due to direct oxidation of ethanol to acetic acid with acetaldehyde as an intermediate. The complete genome sequence of Gluconobacter oxydans 621H provided rich information on the physiology and biochemistry of acetic acid bacteria. The direct oxidations are done by membrane-bound PQQ or flavin dependent enzyme systems with their active sites facing towards the periplasm. The membrane-bound dehydrogenases feed the electrons derived from the oxidations directly into a short electron transport chain that translocates relatively few protons. Besides the membrane-bound dehydrogenases the organism has an additional set of dehydrogenases located in the cytoplasm. They may function mainly in carbon assimilation. The intermediary metabolism seems to be specialized in providing building blocks for biosynthesis. An Entner-Doudoroff pathway and the pentose-phosphate cycle are present; Glycolysis is incomplete due to a missing phosphofructokinase. As succinate thiokinase and succinate dehydrogenase are missing, there is no closed TCA cycle. Furthermore, there is no possibility for the formation of phosphoenolpyruvate from pyruvate and therefore there is no gluconeogenesis from acetate or lactate.
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References
Adachi O, Fujii Y, Ano Y, Moonmangmee D, Toyama H, Shinagawa E, Theeragool G, Lotong N, Matsushita K (2001) Membrane-bound sugar alcohol dehydrogenase in acetic acid bacteria catalyzes l-ribulose formation and NAD-dependent ribitol dehydrogenase is independent of the oxidative fermentation. Biosci Biotechnol Biochem 65:115–125
Adachi O, Moonmangmee D, Toyama H, Yamada M, Shinagawa E, Matsushita K (2003) New developments in oxidative fermentation. Appl Microbiol Biotechnol 60:643–653
Anthony C (1996) Quinoprotein-catalysed reactions. Biochem J 320(Pt 3):697–711
Attwood MM, van Dijken JP, Pronk JT (1991) Glucose metabolism and gluconic acid production by Acetobacter diazotrophicus. J Ferment Bioeng 72:101–105
Barbe JC, De Revel G, Joyeux A, Bertrand A, Lonvaud-Funel A (2001) Role of botrytized grape micro-organisms in SO2 binding phenomena. J Appl Microbiol 90:34–42
Bartowsky EJ, Henschke A (2008) Acetic acid bacteria spoilage of bottled red wine-A review. Int J Food Microbiol 125(1):60–70
Bartowsky EJ, Xia D, Gibson RL, Fleet GH, Henschke PA (2003) Spoilage of bottled red wine by acetic acid bacteria. Lett Appl Microbiol 36:307–314
Beppu T (1993) Genetic organization of Acetobacter for acetic acid fermentation. Antonie Van Leeuwenhoek 64:121–135
Chen C, Liu BY (2000) Changes in major components of tea fungus metabolites during prolonged fermentation. J Appl Microbiol 89:834–839
Cho JJ, Hayward C, Rohrbach KG (1980) Nutritional requirements and biochemical activities of pineapple pink disease bacterial strains from Hawaii. Antonie Van Leeuwenhoek 46:191–204
Davidson VL (2004) Electron transfer in quinoproteins. Arch Biochem Biophys 428:32–40
De Ley J (1963) Comparative carbohydrate metabolism and a proposal for a phylogenetic relationship of the acetic acid bacteria. J Gen Microbiol 24:31–50
De Ley J, Gillis M, Swings J (1984) Family VI. Acetobacteraceae. In: NR Krieg and JG Holt (ed.), Bergey's Manual of Systematic Bacteriology, vol. 1. The Wlliams & Wilkins, Baltimore, MD, pp. 267–278
Deppenmeier U, Ehrenreich A (2008) Physiology of acetic acid bacteria in light of the genome sequence of Gluconobacter oxydans. J Mol Microbiol Biotechnol 16:69–80
Deppenmeier U, Hoffmeister M, Prust C (2002) Biochemistry and biotechnological applications of Gluconobacter strains. Appl Microbiol Biotechnol 60:233–242
Dittrich HH (1987) Mikrobiologie des Weines, 2 ed. Ulmer, Stuttgart
Drysdale GS, Fleet GH (1988) Acetic acid bacteria in winemaking: a review. Am J Enol Vitic 39:143–154
Elfari M, Ha W, Bremus C, Merfort M, Khodaverdi V, Herrmann U, Sahm H, Görisch H (2005) A Gluconobacter oxydans mutant converting glucose almost quantitatively to 5-keto-d-glu- conic acid. Appl Microbiol Biotechnol 66:668–674
Fomenkov A, Xiao JP, Xu SY (1995) Nucleotide sequence of a small plasmid isolated from Acetobacter pasteurianus. Gene 158:143–144
Goodwin PM, Anthony C (1998) The biochemistry, physiology and genetics of PQQ and PQQ- containing enzymes. Adv Microb Physiol 40:1–80
Gosselé F, Swings J, De Ley J (1980) Growth factor requirements of Gluconobacter. Zentralbl Bakteriol Parasitenkd Infekionskr Hyg Abt I Orig Ser C 1:348–350
Greenwalt CJ, Steinkraus KH, Ledford A (2000) Kombucha, the fermented tea: microbiology, composition, and claimed health effects. J Food Prot 63:976–981
Gupta A, Singh K, Qazi N, Kumar A (2001) Gluconobacter oxydans: its biotechnological applications. J Mol Microbiol Biotechnol 3:445–456
Hölscher T, Görisch H (2006) Knockout and overexpression of pyrroloquinoline quinone biosyn- thetic genes in Gluconobacter oxydans 621H. J Bacteriol 188:7668–7676
Hölscher T, Weinert-Sepalage D, Görisch H (2007) Identification of membrane-bound quinoprotein inositol dehydrogenase in Gluconobacter oxydans ATCC 621H. Microbiology 153:499–506
Hoshino T, Sugisawa T, Shinjoh M, Tomiyama N, Miyazaki T (2003) Membrane-bound d-sorbitol dehydrogenase of Gluconobacter suboxydans IFO 3255 — enzymatic and genetic characterization. Biochim Biophys Acta 1647:278–288
Joyeux A, Lafon-Lafourcade S, Ribereau-Gayon P (1984) Evolution of acetic acid bacteria during fermentation and storage of wine. Appl Environ Microbiol 48:153–156
Kashima Y, Nakajima Y, Nakano T, Tayama K, Koizumi Y, Udaka S, Yanagida F (1999) Cloning and characterization of ethanol-regulated esterase genes in Acetobacter pasteurianus. J Biosci Bioeng 87:19–27
Kersters K, De Ley J (1968) The occurence of the Entner–Doudoroff pathway in bacteria. Antonie van Leeuwenhoek J Microbiol Serol 34:393–408
Klasen R, Bringer-Meyer S, Sahm H (1995) Biochemical characterization and sequence analysis of the gluconate:NADP 5-oxidoreductase gene from Gluconobacter oxydans. J Bacteriol 177:2637–2643
Kondo K, Horinouchi S (1997a) Characterization of an insertion sequence, IS12528, from Gluconobacter suboxydans. Appl Environ Microbiol 63:1139–1142
Kondo K, Horinouchi S (1997b) Characterization of the genes encoding the three-component membrane-bound alcohol dehydrogenase from Gluconobacter suboxydans and their expression in Acetobacter pasteurianus. Appl Environ Microbiol 63:1131–1138
Kondo K, Horinouchi S (1997c) A new insertion sequence IS1452 from Acetobacter pasteur- ianus. Microbiology 143(Pt 2):539–546
Kondo K, Beppu T, Horinouchi S (1995) Cloning, sequencing, and characterization of the gene encoding the smallest subunit of the three-component membrane-bound alcohol dehydroge- nase from Acetobacter pasteurianus. J Bacteriol 177:5048–5055
Krahulec J, Kretova M, Grones J (2003) Characterisation of plasmids purified from Acetobacter pasteurianus 2374. Biochem Biophys Res Commun 310:94–97
Lambert B, Kersters K, Gossele F, Swings J, De Ley J (1981) Gluconobacters from honey bees. Antonie van Leeuwenhoek 47:147–157
Leisinger T (1965) Untersuchungen zur Systematik und Stoffwechsel der Essigsäurebakterien. Zentbl Bakteriol Parasitenkd Infktionskrankh Hyg Abt II:329–376
Macauley S, McNeil B, Harvey LM (2001) The genus Gluconobacter and its applications in biotechnology. Crit Rev Biotechnol 21:1–25
Mahillon J, Chandler M (1998) Insertion sequences. Microbiol Mol Biol Rev 62:725–774
Matsushita K, Shinagawa E, Adachi O, Ameyama M (1987) Purification and characterization of cytochrome o-type oxidase from Gluconobacter suboxydans. Biochim Biophys Acta 894:304–312
Matsushita K, Nagatani Y, Shinagawa E, Adachi O, Ameyama M (1989) Effect of extracellular pH on the respiratory chain and energetics of Gluconobacter suboxydans. Agric Biol Chem 36:247–301
Matsushita K, Takahashi K, Takahashi M, Ameyama M, Adachi O (1992) Methanol and ethanol oxidase respiratory chains of the methylotrophic acetic acid bacterium, Acetobacter methano- licus. J Biochem 111:739–747
Matsushita K, Toyama H, Adachi O (1994) Respiratory chains and bioenergetics of acetic acid bacteria. Adv Microb Physiol 36:247–301
Matsushita K, Fujii Y, Ano Y, Toyama H, Shinjoh M, Tomiyama N, Miyazaki T, Sugisawa T, Hoshino T, Adachi O (2003) 5-keto-d-gluconate production is catalyzed by a quinoprotein glycerol dehydrogenase, major polyol dehydrogenase, in Gluconobacter species. Appl Environ Microbiol 69:1959–1966
Neijssel OM (1987) PQQ-linked enzymes in enteric bacteria. Microbiol Sci 4:87–90
Parmentier S, Arnaut F, Soetaert W, Vandamme EJ (2003) Application of NAD-dependent polyol dehydrogenases for enzymatic mannitol/sorbitol production with coenzyme regeneration. Commun Agric Appl Biol Sci 68:255–262
Passmore SM, Carr JG (1975) The ecology of acetic acid bacteria with particular reference to cider manufacture. J Appl Bacteriol 38:151–158
Pronk JT, Levering R, Olijve W, Van Dijkin JP (1989) Role of NADP-dependent and quinoprotein glucose dehydrogenases in gluconic acid production by Gluconobacter oxydans. Enzyme Micob Technol 11:160–164
Prust C (2004) Entschlüsselung des Genoms von Gluconobacter oxydans 621H — einem Bakterium von industriellem Interesse. Georg-August University, Göttingen,Thesis
Prust C, Hoffmeister M, Liesegang H, Wiezer A, Fricke F, Ehrenreich A, Gottschalk G, Deppenmeier U (2005) Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans. Nat Biotechnol 23:195–200
Reid MF, Fewson CA (1994) Molecular characterization of microbial alcohol dehydrogenases. Crit Rev Microbiol 20:13–56
Rojas V, Gil J V, Pinaga F, Manzanares P (2003) Acetate ester formation in wine by mixed cultures in laboratory fermentations. Int J Food Microbiol 86:181–188
Ross P, Mayer R, Benziman M (1991) Cellulose biosynthesis and function in bacteria. Microbiol Rev 55:35–58
Sauer U, Canonaco F, Heri S, Perrenoud A, Fischer E (2004) The soluble and membrane-bound transhydrogenases UdhA and PntAB have divergent functions in NADPH metabolism of Escherichia coli. J Biol Chem 279:6613–6619
Schweiger P, Volland S, Deppenmeier U (2007) Overproduction and characterization of two distinct aldehyde-oxidizing enzymes from Gluconobacter oxydans 621H. J Mol Microbiol Biotechnol 13:147–155
Shibata T, Ichikawa C, Matsuura M, Takata Y, Noguchi Y, Saito Y, Yamashita M (2000) Cloning of a gene for d-sorbitol dehydrogenase from Gluconobacter oxydans G624 and expression of the gene in Pseudomonas putida IFO3738. J Biosci Bioeng 89:463–468
Shinjoh M, Tazoe M, Hoshino T (2002a) NADPH-dependent l-sorbose reductase is responsible for l-sorbose assimilation in Gluconobacter suboxydans IFO 3291. J Bacteriol 184:861–863
Shinjoh M, Tomiyama N, Miyazaki T, Hoshino T (2002b) Main polyol dehydrogenase of Gluconobacter suboxydans IFO 3255, membrane-bound d-sorbitol dehydrogenase, that needs product of upstream gene, sldB, for activity. Biosci Biotechnol Biochem 66:2314–2322
Sievers M, Swings J (2005) Family II. Acetobacteraceae. In: GM Garrity (ed.), Bergey's Manual of Systematic Bacteriology, vol. 2c. Springer, New York, pp. 41–95
Soemphol W, Toyama H, Moonmangmee D, Adachi O, Matsushita K (2007) l-Sorbose reductase and its transcriptional regulator involved in l-sorbose utilization of Gluconobacter frateurii. J Bacteriol 189:4800–4808
Stouthamer AH, van Boom JH, Bastiaanse AJ (1963) Metabolism of C2 compounds in Acetobacter aceti. Antonie van Leeuwenhoek J Microbiol Serol 29:393–406
Tamaki T, Horinouchi S, Fukaya M, Okumura H, Kawamura Y, Beppu T (1989) Nucleotide sequence of the membrane-bound aldehyde dehydrogenase gene from Acetobacter polyoxo- genes. J Biochem 106:541–544
Toyama H, Mathews FS, Adachi O, Matsushita K (2004) Quinohemoprotein alcohol dehydroge- nases: structure, function, and physiology. Arch Biochem Biophys 428:10–21
Toyama H, Soemphol W, Moonmangmee D, Adachi O, Matsushita K (2005) Molecular properties of membrane-bound FAD-containing d-sorbitol dehydrogenase from thermotolerant Gluconobacter frateurii isolated from Thailand. Biosci Biotechnol Biochem 69:1120–1129
Trcek J, Raspor P, Teuber M (2000) Molecular identification of Acetobacter isolates from submerged vinegar production, sequence analysis of plasmid pJK2-1 and application in the development of a cloning vector. Appl Microbiol Biotechnol 53:289–295
Valla S, Coucheron DH, Kjosbakken J (1987). The plasmids of Acetobacter xylinum and their interaction with the host chromosome. Mol Gen Genet 208:76–83
Verma V, Felder M, Cullum J, Qazi GN (1994) Characterisation of plasmids from diketogluconic acid producing strains of Gluconobacter oxydans. J Biotechnol 36:85–88
White GA, Wang CH (1964a) The dissimilation of glucose and glconate by Acetobacter xylinum. 1. The origin and fate of triose phosphate. Biochem J 90:408–423
White GA, Wang CH (1964b) The dissimilation of glucose and gluconate by Acetobacter xylinum. 2. Pathway evaluation. Biochem J 90:424–433
Wong HC, Fear AL, Calhoon RD, Eichinger GH, Mayer R, Amikam D, Benziman M, Gelfand DH, Measde JH, Emerick AW, Bruner R, Ben-Bassat A, Tal R (1990) Genetic organization of the cellulose synthase operon in Acetobacter xylinum. Proc Natl Acad Sci U S A 87:8130–8134
Yamada Y, Yukphan P (2008) Genera and species in acetic acid bacteria. Int J Food Microbiol 125(1):15–24
Yamada Y, Hoshino K, Ishikawa T (1997) The phylogeny of acetic acid bacteria based on the partial sequences of 16S ribosomal RNA: the elevation of the subgenus Gluconoacetobacter to the generic level. Biosci Biotechnol Biochem 61:1244–1251
Yamada M, Elias MD, Matsushita K, Migita CT, Adachi O (2003) Escherichia coli PQQ-contain- ing quinoprotein glucose dehydrogenase: its structure comparison with other quinoproteins. Biochim Biophys Acta 1647:185–192
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Ehrenreich, A. (2009). The Genome of Acetic Acid Bacteria. In: König, H., Unden, G., Fröhlich, J. (eds) Biology of Microorganisms on Grapes, in Must and in Wine. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-85463-0_21
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