Skip to main content

Manipulating and imaging molecular motors with optical traps, single-molecule fluorescence and atomic force microscopy

  • Conference paper
EMC 2008 14th European Microscopy Congress 1–5 September 2008, Aachen, Germany

Abstract

Motor proteins move cargos in cells and are involved in coordinated large-scale force generation e.g. in cell locomotion, cell division or muscle contraction. Optical traps in conjunction with high-resolution light microscopy has been a tool of choice to analyze dynamic properties of motors, such as power strokes, step lengths or unitary forces. Much detail about microscopic function has also been learned by direct imaging.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. L. C. Kapitein, E. J. G. Peterman, B. H. Kwok, J. H. Kim, T. M. Kapoor, C. F. Schmidt, Nature, 435 (2005), p. 114–118.

    Article  ADS  Google Scholar 

  2. B. H. Kwok, L. C. Kapitein, J. H. Kim, E. J. G. Peterman, C. F. Schmidt, T. M Kapoor, Nat. Chem. Biol., 2 (2006), p. 480–485.

    Article  Google Scholar 

  3. M. J.Korneev, S. Lakamper, C. F. Schmidt, European Biophys. J./Biophys. Lett., 36 (2007), p. 675–681.

    Article  Google Scholar 

  4. P. J. de Pablo, I. A. T. Schaap, F. C. MacKintosh, C. F. Schmidt, Phys. Rev. Lett., 91 (2003), 098101.

    Article  ADS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2008 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Schmidt, C.F. et al. (2008). Manipulating and imaging molecular motors with optical traps, single-molecule fluorescence and atomic force microscopy. In: Aretz, A., Hermanns-Sachweh, B., Mayer, J. (eds) EMC 2008 14th European Microscopy Congress 1–5 September 2008, Aachen, Germany. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-85228-5_109

Download citation

Publish with us

Policies and ethics