Abstract
Tissue engineering is a technology which provides tissues for replacing damaged tissues and organs for functional restoration. Collagen, as a basic substance in this field, can be divided into several types, namely type I and type II collagen are studied in this work. Type I collagen is present in fibrous tissues (e.g. tendon) and fibrocartilage, whereas type II collagen proves hyaline character of cartilage [1]. Their relative abundance in cartilage tissues is related to age, degeneration rate, and other qualities of a sample [2]. The goal of our experimental work is to explore the capabilities of Second Harmonic Generation (SHG) Imaging and Two Photon Excitation Microscopy (TPEM) to distinguish type I and type II collagen.
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References
J.A. Buckwalter, H.J. Mankin: Articular cartilage: tissue design and chondrocyte-matrix interactions. Instr Course Lect 47 (1998a), pp. 477–486 our first reference enters here in the style given in [2,3].
J.A. Buckwalter, H.J. Mankin: Articular cartilage: degeneration and osteoarthritis, repair, regeneration, and transplantation. Instr Course Lect 47 (1998b), pp. 487–504
The presented study was supported by the Academy of Sciences of the Czech Republic (Research projects No. AV0Z50110509, AV0Z 50390512, and AV0Z 50390703), Ministry of Education, Youth and Sports of the Czech Republic (research program LC06063, NPV II 2B06130, MSM6840770012), Grant Agency of the Czech Republic (No. 102/08/0691), and Grant Agency of the Academy of Sciences No. IAA500390702.
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Burdíková, Z. et al. (2008). Two photon excitation microscopy and SHG imaging as a tool for visualisation of type I and type II collagen, and their use in tissue engineering. In: Aretz, A., Hermanns-Sachweh, B., Mayer, J. (eds) EMC 2008 14th European Microscopy Congress 1–5 September 2008, Aachen, Germany. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-85228-5_100
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DOI: https://doi.org/10.1007/978-3-540-85228-5_100
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