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Some Aspects of Doping and Medication Control in Equine Sports

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Doping in Sports: Biochemical Principles, Effects and Analysis

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 195))

Abstract

This chapter reviews drug and medication control in equestrian sports and addresses the rules of racing, the technological advances that have been made in drug detection and the importance of metabolism studies in the development of effective drug surveillance programmes. Typical approaches to screening and confirmatory analysis are discussed, as are the quality processes that underpin these procedures. The chapter also addresses four specific topics relevant to equestrian sports: substances controlled by threshold values, the approach adopted recently by European racing authorities to control some therapeutic substances, anabolic steroids in the horse and LC–MS analysis in drug testing in animal sports and metabolism studies. The purpose of discussing these specific topics is to emphasise the importance of research and development and collaboration to further global harmonisation and the development and support of international rules.

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References

  • Aguilera R, Becchi M, Mateus L, Popot MA, Bonnaire Y, Casabiance H, Hatton CK (1997) Detection of exogenous hydrocortisone in horse urine by gas chromatography-combustion-carbon isotope ratio mass spectrometry. J Chromatogr B 702:85–91

    Article  Google Scholar 

  • Aguilera R, Catlin DH, Becchi M, Phillips A, Wang C, Swerdloff RS, Pope HG, Hatton CK (1999) Screening urine for exogenous testosterone by isotope ratio mass spectrometric analysis of one pregnanediol and two androstanediols. J Chromatogr B 727:95–105

    Article  Google Scholar 

  • Arthur RM, Cohen ND, Stanley SD, Wang N (2007) Performance and other factors associated with pre-race serum total CO2 concentrations in thoroughbreds. In: Houghton E, Kijima-Suda I, Wada R, Wade JF (eds) Proc 16th Int Conf Racing Anal Vet. R&W Communications, Newmarket, pp 292–293

    Google Scholar 

  • Auer DE, Skelton KV, Tay S, Baldock FC (1993) Detection of bicarbonate administration (milkshake) in Standardbred horses. Aust Vet J 70:336–340

    Article  Google Scholar 

  • Auer DE, Hughes K, Vine J, McCaffrey J, Duffield AM, Suann CJ, Russo CI, Symons P, Blight GW, Reilly P (2001) Measurement of total plasma carbon dioxide concentrations in thoroughbreds using ASE linearity set. In: Williams RB, Houghton E, Wade JF (eds) Proc 13th Int Conf Racing Anal Vet, R&W Publications, Newmarket, pp 142–114

    Google Scholar 

  • Beaumier PM (1995) Salicylate threshold. In: Tobin T, Mundy GD, Stanley SD, Sams RA, Crone DL (eds) Testing for therapeutic medications, environment and dietary substances in racing horses. The Maxwell H. Gluck Equine Research Center, Lexington, Kentucky, USA, pp 61–63

    Google Scholar 

  • Beaumier PM, Fenwick JD, Stevenson AJ, Weber M, Young LM (1987) Presence of salicyclic acid in Standardbred horse urine and plama after various feed and drug administrations. Equine Vet J 19:207–213

    Article  Google Scholar 

  • Brooks L, Stevenson AJ, Weber MW (2001) Effects of sampling and analysis times and furosemide administration on TCO2 concentrations in standardbred and thoroughbred horses. In: Williams RB, Houghton E, Wade JF (eds) Proc 13th Int Conf Racing Anal Vet, R&W Publications, Newmarket, pp 368–337

    Google Scholar 

  • Collins E, Palmer L, Lewis R, Lewin P (2005) Establishment of average natural total plasma carbon dioxide levels in State of Maine racehorses. In: Albert PH, Morton T, Wade JF (eds) Proc 15th Int Conf Racing Anal Vet, R&W Publications, Newmarket, pp 349–335

    Google Scholar 

  • Crone DL (1995) Arsenic and DMSO thresholds. In: Tobin T, Mundy GD, Stanley SD, Sams RA, Crone DL (eds) Testing for therapeutic medications, environment and dietary substances in racing horses. The Maxwell H. Gluck Equine Research Center, Lexington, Kentucky, USA, pp 63–69

    Google Scholar 

  • De Boer D, Bernal MEG, van Ooyen RD, Maes RA (1991) The analysis of trenbolone and human urinary metabolites of trenbolone acetate by gas chromatography/mass spectrometry and gas chromatography/tandem mass spectrometry. Biol Mass Spectrom 20:459–466

    Article  Google Scholar 

  • Dehennin L, Bonnaire Y, Plou P, Ho ENM, Yiu KCH, Wan TSM (2003) Urinary excretion of endogenous boldenone by entire male horses: Identification and quantification by gas chromatography–mass spectometry. In: Hill DW, Hill WT (eds) Proceedings of the 14th Int Conf Racing Anal Vet. R & W Publications, Newmarket, pp 58–64

    Google Scholar 

  • Dehennin L, Bonnaire Y, Plou P (2007) Detection of nandrolone administration to the entire male horse by a provisional concentration threshold for urinary oestranediol determined by gas chromatography-mass spectrometry. Equine Vet J 39(2):186–188

    Article  Google Scholar 

  • Dumasia MC (2003) In vivo biotransformation of 17 alpha-methyltestosterone in the horse revisited: identification of 17-hydroxymethyl metabolites in equine urine by capillary gas chromatography/mass spectrometry. Rapid Commun Mass Spectrom 17:320–329

    Article  Google Scholar 

  • Dumasia MC, Houghton E (1981) Studies related to the Metabolism of anabolic steroids in the horse: The identification of some 16-oxygenated metabolites of testosterone and a study of the phase II metabolism. Xenobiotica 11:323–331

    Article  Google Scholar 

  • Dumasia MC, Houghton E (1984) Studies related to the metabolism of anabolic steroids in the horse: The phase I and phase II biotransformation of 19-nortestosterone in the equine castrate. Xenobiotica 14:647–655

    Article  Google Scholar 

  • Dumasia MC, Houghton E (1986) Development of a gas chromatographic mass spectrometric method using multiple analytes for the confirmatory analysis of anabolic steroids in horse urine. I. Detection of Testosterone Phenylpropionate Administrations to Equine Male Castrates. J Chromatogr 377:23–33

    Article  Google Scholar 

  • Dumasia MC, Houghton E (1988) Biotransformation of 1-dehydrotestosterone in the equine male castrate: identification of the neutral unconjugated and glucuronic acid conjugated metabolites in horse urine. Biomed Environ Mass Spectrom 17:383–392

    Article  Google Scholar 

  • Dumasia MC, Houghton E, Bradley CV, Williams DH (1983) Studies related to the metabolism of anabolic steroids in the horse: the metabolism of 1-dehydrotestosterone and the use of fast atom bombardment mass spectrometry in the identification of steroid conjugates. Biomed Mass Spectrom 10:434–440

    Article  Google Scholar 

  • Dumasia MC, Teale P, Armstrong R, Houghton E (1996) LC/MS analysis of intact steroid conjugates: a preliminary study on the quantification of testosterone sulphate in equine urine. In: Auer DE, Houghton E (eds) Proc 11th Int Conf Racing Anal Vet. R & W Publications, Newmarket, pp 188–194

    Google Scholar 

  • Edlund PO, Bowers L, Henion J, Covey TR (1989) Rapid-determination of methandrostenolone in equine urine by isotope-dilution liquid-chromatography tandem mass-spectrometry. J Chromatogr-Biomed Appl 497:49–57

    Article  Google Scholar 

  • Ferchaud V, LeBizec B, Monteau F, Andre FP (1998) Determination of the exogenous character of testosterone in bovine urine by gas chromatography–combustion-isotope ratio mass spectrometry. Analyst 123:2617–2620

    Google Scholar 

  • Ferchaud V, LeBizec B, Monteau F, Andre FP (2000) Characterization of exogenous testosterone in livestock by gas chromatography/combustion/isotope ratio mass spectrometry: influence of feeding and age. Rapid Commun Mass Spectrom 14:652–656

    Article  Google Scholar 

  • Fox J, Dumasia MC, Stanley S, Houghton E (2001) The in vivo transformation of norethandrolone in the horse. In: Williams RB, Houghton E, Wade JF (eds) Proceedings of the 13th Int Conf Racing Anal Vet. R & W Publications, Newmarket, pp 279–286

    Google Scholar 

  • Ginn A, Clark A, Grainger L, Houghton E, Willimas RB (2001) Substances of dietary origin. In: Williams RB, Houghton E, Wade JF (eds) Proceedings of the 13th Int Conf Racing Anal Vet. R & W Publications, Newmarket, pp 355–359

    Google Scholar 

  • Gourdie T, Beresford G (1995) The detection of ethylestrenol and its metabolites in the horse. In: Kallings P, Bondesson U, Houghton E (eds) Proceedings of the 10th international conference of racing analysts and veterinarians. R & W Publications, Newmarket, pp 215–217

    Google Scholar 

  • Grace PB, Drake EC, Teale P, Houghton E (2008) Quantification of 19-nortestosterone sulphate and boldenone sulphate in urine from male horses using liquid chromatography/tandem mass spectrometry. Rapid Commun Mass Spectrom 22(19):2999–3007

    Article  Google Scholar 

  • Guan FY, Uboh CE, Soma LR, Luo Y, Rudy J, Tobin T (2005) Detection, quantification and confirmation of anabolic steroids in equine plasma by liquid chromatography and tandem mass spectrometry. J Chromat B-Anal Technol Biomed Life Sci 829:56–68

    Article  Google Scholar 

  • Guan G, Soma LR, Luo Y, Uboh CE, Peterman S (2006) Collision-induced dissociation pathways of anabolic steroids by electrospray ionisation tandem mass spectrometry. J Am Soc Mass Spectrom 17(4):477–489

    Article  Google Scholar 

  • Guan F, Uboh CE, Soma LR, Birks E, Chen J, Mitchell J, You Y, Rudy J, Xu F, Li X, Mbuy G (2007) LC–MS/MS method for the confirmation of recombinant human erythropoietin and darpepoetin Alpha in equine plasma. Anal Chem 79(12):4627–4635

    Article  Google Scholar 

  • Guan F, Uboh CE, Soma LR, Birks E, Chen J, You Y, Ruby J, Li X (2008) differentiation and identification of recombinant human erythropoietin and darpepoetin Alpha in equine plasma by LC–MS/MS for doping control. Anal Chem 80(10):3811–3817

    Article  Google Scholar 

  • Haywood PE, Teale P, Moss MS (1990) The excretion of theobromine in Thoroughbred racehorses after feeding compounded cubes containing cocoa husk – establishment of a threshold value in horse urine. Equine Vet J 22:244–246

    Article  Google Scholar 

  • Ho ENM, Yiu KCH, Tang FPW, Dehennin L, Plou P, Bonnaire Y, Wan TSM (2004) Detection of endogenous boldenone in the entire male horse. J Chromatogr B 808:287–294

    Article  Google Scholar 

  • Ho ENM, Leung DKK, Wan TSM, Yu NH (2005) Metabolic studies of methenolone acetate in horses. Anal Chim Acta 540:111–119

    Article  Google Scholar 

  • Ho ENM, Leung DKK, Wan TSM, Yu NH (2006) Comprehensive screening of anabolic steroids, corticosteroids, and acidic drugs in horse urine by solid-phase extraction and liquid chromatography–mass spectrometry. J Chromatogr A 1120:38–53

    Article  Google Scholar 

  • Ho ENM, Kwok WH, Leung DK, Wan TS, Wong AS (2007a) Metabolic studies of turinabol in horses. Anal Chim Acta 586(1–2):208–216

    Article  Google Scholar 

  • Ho ENM, Leung DK, Leung GN, Wan TS, Wong HN, Xu X, Yeung JH (2007b) Metabolic studies of mesterolone in horses. Anal Chim Acta 596(1):149–155

    Article  Google Scholar 

  • Horning S, Geyer H, Flenker U, Schanzer W (1998) Detection of exogenous steroids by 13C/12C analysis. In: Schanzer W, Geyer H, Gotzmann A, Mareck-Engelke U (eds) Recent advance in doping analysis (5): Proc 15th cologne workshop on dope analysis. Sport und Buch Strauss, Cologne, pp 135–148

    Google Scholar 

  • Houghton E (1977) Studies related to the metabolism of anabolic steroids in the horse: 19-nortestosterone. Xenobiotica 7:683–693

    Article  Google Scholar 

  • Houghton E (1995) The theobromine and hydrocortisone threshold values. In: Tobin T, Mundy GD, Stanley SD, Sams RA, Crone DL (eds) Testing for therapeutic medications, environment and dietary substances in racing horses. The Maxwell H. Gluck Equine Research Center, Lexington, Kentucky, USA, pp 69–73

    Google Scholar 

  • Houghton E, Dumasia MC (1979) Studies related to the metabolism of anabolic steroids in the horse: testosterone. Xenobiotica 9:269–279

    Article  Google Scholar 

  • Houghton E, Dumasia MC (1980) Studies related to the metabolism of anabolic steroids in the horse: the identification of some 16-oxygenated metabolites of 19-nortestosterone. Xenobiotica 10:381–390

    Article  Google Scholar 

  • Houghton E, Oxley GA, Moss MS, Evans S (1978) Studies related to the metabolism of anabolic steroids in the horse: a gas chromatographic mass spectrometric method to confirm the administration of 19-nortestosterone or its esters to horses. Biomed Mass Spectrom 5:170–173

    Article  Google Scholar 

  • Houghton E, Copsey J, Dumasia MC, Haywood PE, Moss MS, Teale P (1984a) The identification of C-18 neutral steroids in normal stallion urine. Biomed Mass Spectrom 11:96–99

    Article  Google Scholar 

  • Houghton E, Copsey J, Dumasia MC, Haywood PE, Moss MS, Teale P (1984b) The identification of C18 neutral steroids in normal stallion urine. Biomed Mass Spectrom 11:96–99

    Article  Google Scholar 

  • Houghton E, Ginn A, Teale P, Dumasia MC, Moss MS (1986a) Detection of the administration of anabolic preparations of nandrolone to the entire male horse. Equine Vet J 18:493–497

    Article  Google Scholar 

  • Houghton E, Dumasia MC, Teale P, Moss MS, Sinkins S (1986b) Development of a gas chromatographic mass spectrometric method using multiple analytes for the confirmatory analysis of anabolic steroid Residues in horse urine. II. Detection of administration of 19-nortestosterone phenylpropionate to equine male castrates and fillies. J Chromatogr 383:1–8

    Article  Google Scholar 

  • Houghton E, Ginn A, Teale P, Dumasia MC, Copsey J (1989) Comparison of the use of mass spectrometry and methylene unit values in the determination of the sterochemistry of estranediol, the major Urinary metabolite of 19-nortestosterone in the horse. J Chromatogr 479:73–83

    Article  Google Scholar 

  • Houghton E, Ginn A, Hall SE, Horning S, Mason P, Gilmour I, Pillinger C, Seymour MA, Schänzer W (2000) Studies on the administration of human chorionic gonadotrophin to the stallion. In: Laviolette B, Koupai-Abyazani MR (eds) Proc 12th Int Conf Racing Anal Vet. R&W Publications, Newmarket, pp 264–268

    Google Scholar 

  • Houghton E, Teale P, Dumasia MC (2007) Studies related to the origin of C18 neutral steroids isolated from extracts of urine from the male horse: The identification of urinary 19-oic acids and their decarboxylation to produce estr-4-en-17 beta-ol-3-one (19-nortestosterone) and estr-4-ene-3,17-dione (19-norandrost-4-ene-3,17-dione) during sample processing. Anal Chim Acta 586:196–207

    Article  Google Scholar 

  • Irvine CHG (2000) An international bicarbonate threshold–is it feasible? In: Laviolette B, Koupai-Abyazani MR (eds) Proc 12th Int Conf Racing Anal Vet. R&W Publications, Newmarket, pp 27–31

    Google Scholar 

  • Irvine CHG, Jensen RC, Lambert MBT, Crone DL (2000) The proposal to lower the international threshold for carbon dioxide. In: Laviolette B, Koupai-Abyazani MR (eds) Proc 12th Int Conf Racing Anal Vet. R&W Publications, Newmarket, pp 9–12

    Google Scholar 

  • Johansson et al. (1986) Pharmceut Biomed Anal 4(2):171–179

    Google Scholar 

  • Jondorf WR (1977) 19-Nortestosterone, a model for the use of anabolic steroid conjugates in raising antibodies for radioimmunoassay. Xenobiotica 7:671–678

    Article  Google Scholar 

  • Jondorf WR, MacDougall DF (1977) Application of radioimmunoassay method for detecting 19-nortestosterone (nandrolone) in equine and canine plasma. Vet Rec 100:560–562

    Google Scholar 

  • Jondorf WR, Moss MS (1978) Radioimmunoassay technique for detecting urinary excretion products after administration of synthetic anabolic steroids to the horse. Xenobiotica 8:197–206

    Article  Google Scholar 

  • Kallings P, Persson S (1995) Effects of sodium bicarbonate on total carbon dioxide in blood and plasma lactate in the horse. In: Kallings P, Bondesson U, Houghton E (eds) Proc 10th Int Conf Racing Anal Vet. R&W Publications, Newmarket, pp 90–93

    Google Scholar 

  • Kicman AT, Houghton E, Gower DB (2009) Anabolic steroids: Metabolism, doping and detection in human and equestrian sport. In: Makin HJ, Gower DB (eds) Steroid analysis. Springer, Heidelberg (in press)

    Google Scholar 

  • Kim CS, Whittem T, Beresford G (1996) Detection of ethylestrenol in equine urine and plasma. In: Auer DE, Houghton E (eds) Proceedings of the 11th Int Conf Racing Anal Vet. R & W Publications, Newmarket, pp 172–178

    Google Scholar 

  • Kinoshita K, Mori M, Kijima-Suda I, Tanaka K (2007) Estimation of δ13C value of endogenous hydrocortisone in urine from Japanese racehorses and its possible application for dope testing. In: Houghton E, Kimijama-Suda I, Wada R, Wade RF (eds) Proc 16th Int Conf Racing Anal Vet. R & W Publications, Newmarket, pp 508–511

    Google Scholar 

  • Kollias-Baker C, Sams R, Maddy KL (2002) Detection of morphine in blood and urine samples from horses administered poppy seeds and morphine sulphate orally. J Anal Tox 26:81–86

    Google Scholar 

  • Laidler P, Cowan DA, Houghton E, Kicman AT, Marshall DE (1998) Discrimination of mammalian growth hormones by peptide-mass mapping. Rapid Commun Mass Spectrom 12(14):975–981

    Article  Google Scholar 

  • Leung GNW, Ho ENM, Leung DKK, Tang FPW, Wan TSM, Yeung JHK, Wong HNC (2005a) Metabolic studies of clostebol acetate in horses. Chromatographia 61:397–402

    Article  Google Scholar 

  • Leung GNW, Ho ENM, Leung DKK, Tang FPW, Yiu KCH, Wan TSM (2005b) Metabolic studies of clostebol acetate and mesterolone in horses. In: Albert PH, Morton T, Wade JF (eds) Proc 15th Int Conf Racing Anal and Vet. R & W Publications, Newmarket, pp 146–154

    Google Scholar 

  • Luo Y, Uboh CE, Soma LR, Guan FY, Rudy JA, Tsang DS (2005) Simultaneous analysis of twenty-one glucocorticosteroids in equine plasma by liquid chromatography/tandem mass spectrometry. Rapid Commun Mass Spectrom 19(10):1245–1256

    Article  Google Scholar 

  • Mason PM, Hall SE, Gilmour I, Houghton E, Pillinger CT, Seymour M (1998) The use of stable carbon isotope analysis to detect the abuse of testosterone in cattle. Analyst 123:2405–2408

    Article  Google Scholar 

  • McKinney AR, Ridley DD, Suann CJ (2001a) Metabolism of methandrostenolone in the horse: a gas chromatographic–mass spectrometric investigation of phase I and phase II metabolism. J Chromatogr B 765:71–79

    Article  Google Scholar 

  • McKinney AR, Ridley DD, Suann CJ (2001b) The metabolism of norethandrolone in the horse: characterization of 16-, 20- and 21-oxygenated metabolites by gas chromatography/mass spectrometry. J Mass Spectrom 36:145–150

    Article  Google Scholar 

  • McKinney AR, Suann CJ, Dunstan AJ, Mulley SL, Ridley DD, Stenhouse AM (2004) Detection of stanozolol and its metabolites in equine urine by liquid chromatography-electrospray ionization ion trap mass spectrometry. J Chromatogr B-Anal Technol Biomed Life Sci 811:75–83

    Google Scholar 

  • McKinney AR, Suann CJ, Stenhouse AM (2007) A stereochemical examination of the equine metabolism of 17alpha-methyltestosterone. Anal Chim Acta 581(2):377–387

    Article  Google Scholar 

  • Muck WM, Henion JD (1990) High-performance liquid-chromatography tandem mass-spectrometry – its use for the identification of stanozolol and its major metabolites in human and equine urine. Biomed Environ Mass Spectrom 19:37–51

    Article  Google Scholar 

  • Moss MS, Blay P, Houghton E, Horner MW, Teale P, Smith RL, Sloan T (1987) Normal and post-administration concentrations of salicyclic acid in Thoroughbred horses. In: Crone DL (ed) Proc 6th Int Conf Racing Anal Vet. McMillan Publishers, Hong

    Google Scholar 

  • Popot MA, Houghton E, Ginn A, Jones M, Teale P, Samuels T, Lassourd V, Dunnett N, Cowan DA, Bonnaire Y, Toutain PL (1997) Cortisol concentrations in post competition horse urine: A French and British survey. Equine Vet J 29:226–229

    Article  Google Scholar 

  • Pottier J, Cousty C, Heitzman RJ, Reynolds IP (1981) Differences in the biotransformation od a 17 beta-hydroxylated steroid, trenbolone acetate in rat and cow. Xenobiotica 11(7):489–500

    Article  Google Scholar 

  • Reilly P, Duffield A, Suann CJ, Vine J, Batty D, Auer D, Skelton K, Tay S, Stenhouse A, Ralston J (1996) The analysis of plasma total carbon dioxide in racehorses. In: Auer DE, Houghton E (eds) Proc 11th Int Conf Racing Anal Vet, R & W Publications, Newmarket, pp 2387–2240

    Google Scholar 

  • Scarth J, Davis M, Houghton R, Houghton E, Williams RB, Webbon PM (2001) The effect of sodium bicarbonate administration on equine plasma total carbon dioxide concentrations. In: Williams RB, Houghton E, Wade JF (eds) Proc 13th Int Conf Racing Anal Vet. R&W Publications, Newmarket, pp 148–151

    Google Scholar 

  • Schanzer W, Opfermann G, Donike M (1990) Metabolism of stanozolol: identification and synthesis of urinary metabolites. J Steroid Biochem 36:153–174

    Article  Google Scholar 

  • Schoene C, Nedderman ANR, Houghton E (1994) Preliminary-study of the metabolism of 17-alpha-methyltestosterone in horses utilizing gas-chromatography – mass-spectrometric techniques. Analyst 119:2537–2542

    Google Scholar 

  • Schuback K, Kallings P, Bondesson U, Essen-Gustavsson B, Persson S (2000) Effect of sodium bicarbonate treatment on anaerobic metabolism and total carbon dioxide in plasma during post exercise recovery. In: Laviolette B, Koupai-Abyazani MR (eds) Proc 12th Int Conf Racing Anal Vet. R&W Publications, Newmarket, pp 14–19

    Google Scholar 

  • Scott KR, Lambert MB, Field L, Kavanagh PV (2005) The detection and quantitation of morphine in horse feeds and opium poppies – the Irish experience. In: Albert PH, Morton T, Wade JF (eds) Proc 15th Int Conf Racing Anal Vet. R&W Communications, Newmarket, pp 40–45

    Google Scholar 

  • Smith RL (1987) Report on standardising policies in Europe. In: Crone DL (ed) Proc 6th Int Conf Racing Anal Vet. McMillan Publishers, Hong Kong, pp 402–404

    Google Scholar 

  • Soma LR, Uboh CE, Guan F, McDonnell S, Pack J (2007) Pharmacokinetics of boldenone and stanozolol and the results of quantification of anabolic and androgenic steroids in race horses and nonrace horses. J Vet Pharmacol Ther 30(2):101–108

    Article  Google Scholar 

  • Soma LR, Uboh CE, Guan F, McDonnell S (2008) Plasma concentrations of testosterone and 19-nortestosterone (nandrolone) in nonracing intact male horses by liquid chromatography–mass spectrometry. J Vet Pharmacol Ther 31(6):587–590

    Article  Google Scholar 

  • Stanley SM, Smith L, Rodgers JP (1997a) Biotransformation of 17-alkyl steroids in the equine: gas chromatographic–mass spectral identification of ten intermediate metabolites of methyltestosterone. J Chromatogr B-Biomed Sci Appl 390(1–2):55–64

    Article  Google Scholar 

  • Stanley SM, Kent S, Rodgers JP (1997b) Biotransformation of 17-alkyl steroids in the equine: high-performance liquid chromatography mass spectrometric and gas chromatography mass spectrometric analysis of fluoxymesterone metabolites in urine samples. J Chromatogr B–Anal Technol Biomed Life Sci 704:119–128

    Article  Google Scholar 

  • Stanley SM, Wee WK, Lim BH, Foo HC (2007) Direct-injection screening for acidic drugs in plasma and neutral drugs in equine urine by differential-gradient LC–LC coupled MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci 848(2):292–302

    Article  Google Scholar 

  • Stenhouse AM, Bryce AJ, Andrew AS, Suann CJ, Mulley SL, McKinney AR, Rogerson J (2007) Analytical confirmation of sodium bicarbonate administrations to horses using carbon isotope ratio mass spectrometry (CIRMS). In: Houghton E, Kijima-Suda I, Wada R, Wade JF (eds) Proc 16th Int Conf Racing Anal Vet. R&W Communications, Newmarket, pp 206–212

    Google Scholar 

  • Tang PW, Crone DL (1995) Validation of Australia’s data for plasma bicarbonate. In: Kallings P, Bondesson U, Houghton E (eds) Proc 10th Int Conf Racing Anal Vet. R&W Publications, Newmarket, pp 327–328

    Google Scholar 

  • Tang PW, Law WCL, Wan TS M, Crone DL (2000) Metabolic studies of some oral anabolic steroids in the horse: part 1, oxymetholone and mestanolone. In: Laviolette B, Koupai-Abyazani MR (eds) Proc 12th Int Conf Racing Anal Vet. R & W Publications, Newmarket, pp 118–124

    Google Scholar 

  • Tang FPW, Watkins KL, Wan TSM (2001) Metabolic studies of some oral anabolic steroids in the horse: part 2, danazol. In: Williams RB, Houghton E, Wade JF (eds) Proc13th Int Conf Racing Anal Vet. R & W Publications, Newmarket, pp 171–178

    Google Scholar 

  • Teale P, Houghton E (1991) The development of a gas chromatographic/mass spectrometric screening procedure to detect the administration of anabolic steroids to the horse. Biolog Mass Spec 20:109–114

    Article  Google Scholar 

  • Toutain PL, Lassourd V (2002) Pharmacokinetic/pharmacodynamic approach to assess irrelevant plasma or urine drug concentrations in post competition samples for drug control in horses. Equine Vet J 34:242–249

    Article  Google Scholar 

  • Toutain PL, Lassourd V (2003) Pharmacokinetic/pharmacodynamic assessment of irrelevant drug concentrations in horse plasma or urine for a selection of drugs. In: Hill DW, Hill WT (eds) Proc 14th Int Conf Racing Anal Vet, R & W Publications, Newmarket, pp 19–28

    Google Scholar 

  • Vine J (2000) Plasma total carbon dioxide concentrations in racehorses: challenges to test results. In: Laviolette B, Koupai-Abyazani MR (eds) Proc 12th Int Conf Racing Anal Vet. R & W Publications, Newmarket, pp 32–36

    Google Scholar 

  • Vonparti A, Lyris E, Panderi I, Koupparis M, Georgakopoulos C (2008) Direct injection LC/ESI-MS horse urine analysis for the quantification and identification of threshold substances for doping control 1. Determination of hydrocortisone. J Mass Spectrom 43(9):1255–1264

    Article  Google Scholar 

  • Wynne PM (2005) The accidental contamination of animal feed by naturally occurring opiates with particular reference to morphine. An independent review and analysis of current knowledge. The British Equestrian Trade Association (London)

    Google Scholar 

  • Wynne PM, Vine JH, Amiet RG (2001a) The quantitative analysis of equine urine for metabolites of levodopa and dopamine. In: Williams RB, Houghton E, Wade JF (eds) Proc 13th Int Conf Racing Anal Vet. R & W Publications, Newmarket, pp 64–72

    Google Scholar 

  • Wynne PM, McCaffrey JP, Vine JH, Amiet RG (2001b) Precursor loading with tyrosine to increase levodopa and dopamine in the horse. In: Williams RB, Houghton E, Wade JF (eds) Proc13th Int Conf Racing Anal Vet. R & W Publications, Newmarket, pp 73–86

    Google Scholar 

  • Yamada M, Kinoshita K, Kurosawa M, Saito K, Nakazawa H (2007a) Analysis of exogenous nandrolone metabolite in horse urine by gas chromatography/combustion/carbon isotope ratio mass spectrometry. J Pharm Biomed Anal 45(4):654–658

    Article  Google Scholar 

  • Yamada M, Aramaki S, Hosoe T, Kurosawa M, Kijima-Suda I, Saito K, Nakazawa H (2007b) Identification and quantification of metabolites common to 17alpha-methyltestosterone and mestanolone in horse urine. J Pharm Biomed Anal 45(1):125–133

    Article  Google Scholar 

  • Yamada M, Aramaki S, Kurosawa M, Saito K, Nakazawa H (2008a) Detection of urinary metabolites common to structurally related 17alpha-alkyl anabolic steroids in horses and application to doping tests in racehorses: methandienone, methandriol and oxymetholone. J Anal Toxicol 32(5):387–391

    Google Scholar 

  • Yamada M, Aramaki S, Okayasu T, Hosoe T, Kurosawa M, Kijima-Suda I, Saito K, Nakazawa H (2008b) Characterisation and quantification of fluoxymesterone metabolites in horse urine by gas chromatography/mass spectrometry. Anal Sci 24(7):911–914

    Article  Google Scholar 

  • You Y, Uboh CE, Soma LR, Guan F, Li X, Rudy JA, Chen J (2007) Biomarkers of alcohol abise in racehorses by liquid chromarography/tandem mass spectrometry. Rapid Commun Mass Spectrom 21(23):3785–3794

    Article  Google Scholar 

  • Yu NH, Ho ENM, Leung DKK, Wan TSM (2005) Screening of anabolic steroids in horse urine by liquid chromatography-tandem mass spectrometry. J Pharm Biomed Anal 37:1031–1038

    Article  Google Scholar 

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Acknowledgment

With regard to Section 7 of this chapter, An Approach for the Control of Therapeutic Substances in Thoroughbred Horseracing, the authors acknowledge the extensive input into the development of the PK/PD model and its application to the assessment of irrelevant plasma and urinary concentrations of drugs by Professor Pierre-Louis Toutain and his colleagues at the Ecole Nationale Vétérinaire de Toulouse, France.

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Correspondence to Ed Houghton .

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Houghton, E., Maynard, S. (2010). Some Aspects of Doping and Medication Control in Equine Sports. In: Thieme, D., Hemmersbach, P. (eds) Doping in Sports: Biochemical Principles, Effects and Analysis. Handbook of Experimental Pharmacology, vol 195. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-79088-4_17

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