Skip to main content
SpringerLink
Log in

Fat Transfer with Platelet-Rich Plasma for Breast Augmentation

  • Chapter
Breast Augmentation

Abstract

Tissue augmentation with autologous elements is considered the ideal choice for transplantation and volume augmentation surgery. It is well recognized that the ideal graft tissue should offer the standard features of ready availability, low antigenicity, minimal donor site morbidity, predictable and reproducible retention, and avoidance of disease transmission. Gradual standardization of consistent harvesting, manipulation, and transfer protocols is improving the ability to accurately predict volume enhancements and appreciate the long-term survival of the grafted tissues. Platelets actively extrude multiple growth factors that are very important to early wound-healing processes. In response to platelet-to-platelet or platelet-to-connective-tissue contact, the platelet cell membrane is “activated” to release these products from the alpha granules via active extrusion. When these extruded growth factors are released, histones and carbohydrate chains are added to receptor sites, thereby creating their unique chemistries and making the “active” growth factors. Autologous fat grafting offers a viable and safe alternative means of breast augmentation in select patients. Platelet-rich plasma enhances the fat transfer to the breast.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 329.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 419.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  • Alexander RW, Abuzeni PZ: Enhancement of autologous fat transplantation with platelet rich plasma. Am J Cosmet Surg 2001;18:59–70

    Google Scholar 

  • Whitman DH: Platelet gel: an autologous alternative to fibrin glue with applications in oral and maxillofacial surgery. J Oral Maxillofacial Surg 1997;55(11):1294–1299

    Article  CAS  Google Scholar 

  • Marx RE: Platelet-rich plasma: growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Path Oral Radiol Endod 1998;85(6):638–646

    Article  CAS  Google Scholar 

  • Fagrell D, Sverker E, Kniola B: Fat cylinder transplantation: an experimental comparative study of three different kinds of transplants. Plast Reconstr Surg 1996;98(1):90–96

    Article  PubMed  CAS  Google Scholar 

  • Niechajev I, Sevcuk O: Long-term results of fat transplantation: clinical and histologic studies. Plast Reconstr Surg 1994;94(3):496–506

    Article  PubMed  CAS  Google Scholar 

  • Billings E, May JW Jr: Historical review and present status of free fat graft autotransplantation in plastic and reconstructive surgery. Plast Reconstr Surg 1989;83(2):368–381

    Article  PubMed  Google Scholar 

  • Nguyen A, Pasyk KA, Bouvier TN: Comparative study of survival of autologous tissue taken and transplanted by different techniques. Plast Recon Surg 1990;85(3):378–386

    CAS  Google Scholar 

  • Fulton JE, Suarez M, Silverton K, Barnes T: Small volume fat transfer. Dermatol Surg 1998;24(8):857–865

    Article  PubMed  CAS  Google Scholar 

  • Alexander RW: Liposculpture in the superficial plane: closed syringe system for improvement in fat removal and free fat transfer. Am J Cosmet Surg 1994;11:127–134

    Google Scholar 

  • Alexander RW, Sadati K, Corrado A: Platelet-rich plasma (PRP) utilized to promote greater graft volume retention in autologous fat grafting. Am J Cosmet Surg 2006;23(4):203–221

    Google Scholar 

  • Obaji S: Selecting for fat transfer success. Cosmet Surg Times 2006;9(4):26

    Google Scholar 

  • Bircoll M: A nine-year experience with autologous fat transplantation. Am J Cosmet Surg 1992;9(1):55–61

    Google Scholar 

  • Coleman SR: Structural Fat Grafting. St. Louis, Quality Medical Publishing 2004, pp 30–175

    Google Scholar 

  • Shippert R: Autologous fat transfer: eliminating the centrifuge, decreasing lipocyte trauma, and establishing standardization for scientific study. Am J Cosmet Surg 2006;23(1):21–27

    Google Scholar 

  • Ersek RA: Transplantation of purified autologous fat; a three year follow-up is disappointing. Plast Recon Surg 1991;87(2):219–227

    CAS  Google Scholar 

  • Pinski KS, Roenigk HH : Autologous fat transplantation long-term followup. J Dermatol Surg Oncol 1992;18(3)179–184

    PubMed  CAS  Google Scholar 

  • Lyles MB: Adipocytes hearty: certain storage conditions favor pre-adipocyte survival. Cosmet Surg Times 2003;6:10–11

    Google Scholar 

  • Coleman SR: Structural fat grafting: more than a permanent filler. Plast Reconstr Surg 2006;118(3 suppl):108S–120S

    Article  PubMed  CAS  Google Scholar 

  • Forgue-Lafitte ME, Van RL, De Gasquet P, Rosselin G: The presence of epidermal growth factor receptors in cultured human adipocyte precursors. Scand J Clin Lab Invest 1982;42(8):627–631

    Article  PubMed  CAS  Google Scholar 

  • Lau D, Roncari D, Yip D, Kindler S, Nilsern S: Purification of a pituitary polypeptide that stimulates the replication of adipocyte precursors in culture. FEBS Lett 1983;153:395–398

    Article  PubMed  CAS  Google Scholar 

  • Zapf J, Schoenle E, Froesch R: Insulin-like growth factor I and II: some biological actions and receptor binding characteristics of two purified constituents of nonsuppressible insulin-like activity of human serum. Eur J Biochem 1978;87(5):285–296

    Article  PubMed  CAS  Google Scholar 

  • Reeder GD, Hood A, Hill A: Perioperative autologous sequestration I: physiology, phenomena, and art. Proceedings of the American Academy of Cardiovascular Perfusion. San Antonio, 1993

    Google Scholar 

  • Haystead T, Hardie DG: Both insulin and epidermal growth factor stimulate lipogenesis and acetyl-CoA carboxylase activity in isolated adipocytes. Inportance of homogenization procedure in avoiding artifacts in acetyl-CoA carboxylase assay. Biochem J 1986;234(2):279–284

    PubMed  CAS  Google Scholar 

  • Ng TB, Wong CM: Epidermal and nerve growth factors manifest antilipolytic and lipogenic activities in isolated rat adipocytes. Comp Biochem Physiol B 1985;81(3):687–689

    CAS  Google Scholar 

  • Chai Y, Salvkin HC: Biology of bone induction and its clinical applications. Oral Maxillofacial Surg Clin 1994;7:739–753

    Google Scholar 

  • Cromak DT: Current concepts in wound healing: growth factor and macrophage interaction. J Trauma 1990;30:130–132

    Google Scholar 

  • Pierce GF: Platelet-derived growth factor-BB and transforming growth factor beta 1 selectively modulate glycosaminoglycans, collagen, and myofibroblasts in excisional wounds. Am J Pathol 1991;138(3):629–646

    PubMed  CAS  Google Scholar 

  • Ross R: The biology of platelet-derived growth factor. Cell 1986;46(2):155–169

    Article  PubMed  CAS  Google Scholar 

  • Pierce GF: Role of platelet-derived growth factor in wound healing. J Cell Biochem 1991;45(4):319–326

    Article  PubMed  CAS  Google Scholar 

  • Eppley BL, Snyders RV, Winkelmann T, Delfino JJ: Autologous facial fat transplantation: improved graft maintenance by microbead bioactivation. J Oral Maxillofacial Surg 1992;50(5):477–482

    Article  CAS  Google Scholar 

  • Jones JK, Lyles M: The viability of human adipocytes after closed-syringe liposuction harvest. Am J Cosmet Surg 1997;14:275–279

    Google Scholar 

  • Marx RE, Carlson E: Growth factor enhancement for bone grafts. J Oral Maxillofacial Surg 1993;51:1181–1193

    Google Scholar 

  • Knighton DR, Hunt TK, Scheuenstuhl H, Halliday BJ, Werb Z, Banda MJ: Oxygen tension regulates the expression of angiogenesis factor by macrophages. Science 1983;221(4617):1283–1285

    Article  PubMed  CAS  Google Scholar 

  • Marx RE, Ehler WJ., Tayapongsak P, Pierce LW: Relationship of oxygen dose to angiogenesis induction in irradiated tissue. Am J Surg 1990;160(5):519–524

    Article  Google Scholar 

  • Carpaneda CA, Ribiero MT: Study of histologic alterations and viability of the adipose graft in humans. Aesth Plast Surg 1993;17(1):43–47

    Article  CAS  Google Scholar 

  • Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrick MH: Multilineage cells from human adipose tissue: implications for cell-based therapies. 2001;7(2):211–228

    Google Scholar 

  • Aust L, Devlin B, Foster SJ, Halvorsen YD, Hicok K, du Laney T, Sen A, Willingmyre GD, Gimble JM: Yield of human adipocyte-derived adult stem cells from liposuction aspirates. Cytotherapy 2004;6(1):7–14

    Article  PubMed  CAS  Google Scholar 

  • Strem BM, Hicok K, Zhu M, Wulur I, Alfonso Z, Schreiber RE, FraserJK, Hedrick MJ: Multipotential differentiation of adipose tissue-derived stem cells. Keio J Med 2005;54(3):132–141

    Article  PubMed  CAS  Google Scholar 

  • Gimble J, Guilak F: Adipose-derived adult stem cells: isolation, characterization, and differentiation potential. Cytotherapy 2003;5(5):362–369

    Article  PubMed  Google Scholar 

  • Fulton JE: Breast contouring with “gelled” autologous fat: 10 year update. Int J Cosmet Surg Aesthet Dermatol 2003;5:155–161

    Article  Google Scholar 

  • Santrach PJ, Hoffman M: Laboratory validation of autologous platelet gel. Transfusion 2004, 44 (12 supp):68A–69A

    Google Scholar 

  • Sacchi MC, Maresca P, Tartuferi L, Bellanda M, Micheletti P, Riva S, Borzini P, Levis A, Rosti G: Platelet gel as a new routine method to improve wound healing and regeneration. Transfus Med 2000;10:325–329

    Article  Google Scholar 

  • Man D, Plosker H, Winland-Brown J.E: The use of autologous platelet-rich plasma (platelet gel) and autologous platelet-poor plasma (fibrin glue) in cosmetic surgery. Plast Reconstr Surg 2001;107(1):229–237

    Article  PubMed  CAS  Google Scholar 

  • Fulton JE: Breast contouring by autologous fat transfer. Am J Cosmet Surg 1992;9:273–279

    Google Scholar 

  • Alexander RW, Maring T, Aghabo T: Autologous fat grafting: a study of residual intracellular adipocyte lidocaine concentrations after serial rinsing with normal saline. Am J Cosmet Surg 1999;16:123–126

    Google Scholar 

  • Ersek RA, Chang P, Salisbury M: A lipo layering of autologous fat: an improved technique with promising results. Plast Reconstr Surg 1998;101(3):820–826

    Article  PubMed  CAS  Google Scholar 

  • Alexander RW: Breast augmentation by injection of autologous fat. Proceedings of the American Society of Cosmetic Breast Surgery, Newport Beach, CA, 1996

    Google Scholar 

  • Alexander RW: Use of fat transfer for facial and breast augmentation. Proceedings of World Congress on Liposuction Surgery, San Francisco 1996

    Google Scholar 

  • Alexander RW: Use of fat grafts for augmentation mammoplasty. Summary of clinical information with radiographic documentation. Proceedings of the American Society of Cosmetic Breast Surgery, Newport Beach, CA, 1998

    Google Scholar 

  • Clark ER, Clark EL: Microscopic studies of new formation of fat in living adult rabbits. Am J Anat 1940;67:255–281

    Article  Google Scholar 

  • Coleman SR: Fat grafting to the breast revisited: safety and efficacy. Plast Reconstr Surg 2007;119(3):775–787

    Article  PubMed  CAS  Google Scholar 

  • Bircoll M: Cosmetic breast augmentation utilizing autologous fat and liposuction techniques. Plast Reconstr Surg 1987;79(2):267–271

    Article  PubMed  CAS  Google Scholar 

  • Bircoll M: Autologous fat transplantation: an evaluation of microcalcification and fat cell survivability following (AFT) cosmetic breast augmentation. Am J Cosmet Surg 1998;5:283–288

    Google Scholar 

  • Hogge JP, Robinson RE, Magnant CM, Zuurbier RA: The mammographic spectrum of fat necrosis of the breast. Radiographics 1995;15(6):1347–1356

    PubMed  CAS  Google Scholar 

  • Johnson GW: Benign breast calcifications. Am J Cosmet Surg 1988;5:177–180

    Google Scholar 

  • Pulagam SR, Poulton T, Mamounas EP: Long-term clinical and radiologic results with autologous fat transplantation for breast augmentation. Breast J 2006;6(1):63–67

    Article  Google Scholar 

  • Fine RE, Staren ED: Updates in breast ultrasound. Surg Clin North Am 2004;84(4):1001–1006

    Article  PubMed  Google Scholar 

  • Chala LF, de Barros N, de Camargo Moraes, P, Endo E, Kim SJ, Pinceraato KM, Carvalho FM, Cerri GG: Fat necrosis of the breast: mammographic, sonographic, computed tomography, and magnetic resonance imaging findings. Curr Probl Diagn Radiol 2004;33(3):106–126

    Article  PubMed  Google Scholar 

  • Kneeshaw PJ, Lowry M, Manton D, Hubbard A, Drew PJ, Turnbull LW: Differentiation of benign from malignant breast disease associated with screening detected microcalcifications using dynamic contract enhanced magnetic resonance imaging. Breast 2006;15(1):29–38

    Article  PubMed  CAS  Google Scholar 

  • Yuksel E, Weinfeld AB, Cleek R, Wamsley S, Jensen J, Boutros S, Waugh JM, Shenaq SM, Spira M: Increased free fat-graft survival with the long-term local delivery of insulin, insulin-like growth factor-1, and basic fibroblast growth factor by PLGA/PEG microspheres. Plast Reconstr Surg 2000;105(5):1712–1720

    Article  PubMed  CAS  Google Scholar 

  • Wabitsch M, Hauner H, Heinze E, Teller WM: The role of growth hormone/insulin-like growth factors in adipocyte differentiation. Metabolism 1995;44(10 suppl 4):45–49

    Article  PubMed  CAS  Google Scholar 

  • Boney CM, Moats-Staats BM, Stiles AD, D’Ercole AJ: Expression of insulin-like growth factor-1 (IGF-1) and IGF-binding proteins during adipogenesis. Endocrinology 1994;135(5):1863–1868

    Article  PubMed  CAS  Google Scholar 

  • Alexander RW: Platelet rich plasma offers vast fat graft benefits. Cosmet Surg Times 2004;7(3):18–20

    Google Scholar 

  • Menschik Z: Vitamin E and adipose tissue. Edinburgh Med J 1944;51:486–489

    CAS  Google Scholar 

  • Sidman RL: The direct effect of insulin on organ cultures of brown fat. Anat Rec 1956;124(4):723–739

    Article  PubMed  CAS  Google Scholar 

  • Reynold AE, Marble A, Fawcett DW: Action of insulin on deposition of glycogen and storage of fat in adipose tissue. Endocrinology 1950;46:55–66

    Google Scholar 

  • Krawisz BR, Scott RE: Coupling of proadipocyte growth arrest and differentiation. I. Induction by heparinized medium containing human plasma. J Cell Biol 1982;94(2):394–399

    Article  PubMed  CAS  Google Scholar 

  • Flores-Delgado G., Marsch-Moreno M, Kuri-Harcuch W: Thyroid hormone stimulates adipocyte differentiation of 3T3 cells. Molec Cell Biochem 1987;76(1):35–43

    PubMed  CAS  Google Scholar 

  • Brandes R, Hertz R, Arad R, Nashtat S, Weil S, Bar-Tana J: Adipocyte conversion of cultured 3T3-L1 pre-adipocytes by benzafibrate. Life Sci 1987;40(10):935–941

    Article  PubMed  CAS  Google Scholar 

  • Ullmann Y, Hyanms M, Ramon Y, Beach D, Peled IJ, Lindenbaum ES: Enhancing survival of aspirated human fat injected into nude mice. Plast Reconstr Surg 1998;101(7):1940–1944

    Article  PubMed  CAS  Google Scholar 

  • Eppley BL, Sidner RA, Platis JM, Sadove MA: Bioactivation of free-fat transfer: a potentially new approach to improving fat survival. Plast Reconstr Surg 1992;90(6):1022–1030

    Article  PubMed  CAS  Google Scholar 

  • Steed DL: Clinical evaluation of recombinant human platelet-derived growth factor for the treatment of lower extremity diabetic ulcers. J Vasc Surg 1995;21(1):79–81

    Article  Google Scholar 

  • Knighton DR, Ciresi K, Fiegel V, Schumerth S, Butler E, Cerra F: The use of topically applied platelet growth factors in chronic non-healing wounds. Wounds 1998;1:71–78

    Google Scholar 

  • Knighton DR, Ciresi K, Fiegel VD, Schumerth S, Butler E, Cerra F: Stimulation of repair in chronic, non-healing cutaneous ulcers using platelet-derived wound healing formula. Surg Gynecol Obstet 1990;170(1):56–60

    PubMed  CAS  Google Scholar 

  • Powell DM, Chang E, Farrior EH: Recovery from deep-plane rhytidectomy following unilateral wound treatment with autologous platelet gel. Arch Plast Surg 2001;3(4):245–250

    Article  CAS  Google Scholar 

  • Khouri RK, Brown DM, Leal-Khouri SM, Tark KC, Shaw WW: The effect of basic fibroblast growth factor on the neovascularization process: skin flap survival and staged flap transfers. Br J Plast Surg 1991;44(8):585–588

    Article  PubMed  CAS  Google Scholar 

  • Alexander RW: Results are satisfying using autologous fat: breast augmentation with autologous fat transfer. Cosmet Surg Times 1998;2(4):4

    Google Scholar 

  • Fournier PF: The breast fill. In: Fournier PF (ed). Liposculpture: The Syringe Technique. Paris, Arnette-Blackwell 1991, pp 357–367

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Surgery, University of Washington, 3500 188th St. SW, Suite 670, 98037, Lynnwood, Washington, USA

    Robert W. Alexander

  2. Health Science Center at San Antonio, Department of Surgery, University of Texas, Texas, USA

    Robert W. Alexander

Authors
  1. Robert W. Alexander
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Surgery Section, Tustin Hospital and Medical Center, 17501 Chatham Drive, 92780-2302, Tustin, CA, USA

    Melvin A. Shiffman

Rights and permissions

Reprints and permissions

Copyright information

© 2009 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Alexander, R. (2009). Fat Transfer with Platelet-Rich Plasma for Breast Augmentation. In: Shiffman, M. (eds) Breast Augmentation. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-78948-2_54

Download citation

  • DOI: https://doi.org/10.1007/978-3-540-78948-2_54

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-78947-5

  • Online ISBN: 978-3-540-78948-2

  • eBook Packages: MedicineMedicine (R0)

Publish with us

Policies and ethics

Search

Navigation